Low-salt diet increases NO bioavailability and COX-2 vasoconstrictor prostanoid production in spontaneously hypertensive rats

Travaglia, Teresa Cristina Francischetto; Berger, Rebeca Caldeira Machado; Luz, Marcela Baggio; Furieri, Lorena Barros; Ribeiro, Junior R.F.; Vassallo, Dalton Valentim; Mill, José Geraldo; Stefanon, Ivanita; Vassallo, Paula Frizera

doi:10.1016/j.lfs.2015.12.027

Cited by 7 publications

(2 citation statements)

References 41 publications

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“…Indeed, recent studies have shown that the mechanism of endothelial dysfunction is largely due to the reduced bioavailability of endothelium-derived NO by oxidative stress [ 9 ]. The presence of ROS not only reduces the bioavailability of NO [ 10 ] but also results in the eNOS uncoupling which will result in more ROS formation [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Rosmarinic Acid Alleviates the Endothelial Dysfunction Induced by Hydrogen Peroxide in Rat Aortic Rings via Activation of AMPK

Zhou

et al. 2017

Oxidative Medicine and Cellular Longevity

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Endothelial dysfunction is the key player in the development and progression of vascular events. Oxidative stress is involved in endothelial injury. Rosmarinic acid (RA) is a natural polyphenol with antioxidative, antiapoptotic, and anti-inflammatory properties. The present study investigates the protective effect of RA on endothelial dysfunction induced by hydrogen peroxide (H2O2). Compared with endothelium-denuded aortic rings, the endothelium significantly alleviated the decrease of vasoconstrictive reactivity to PE and KCl induced by H2O2. H2O2 pretreatment significantly injured the vasodilative reactivity to ACh in endothelium-intact aortic rings in a concentration-dependent manner. RA individual pretreatment had no obvious effect on the vasoconstrictive reaction to PE and KCl, while its cotreatment obviously mitigated the endothelium-dependent relaxation impairments and the oxidative stress induced by H2O2. The RA cotreatment reversed the downregulation of AMPK and eNOS phosphorylation induced by H2O2 in HAEC cells. The pretreatment with the inhibitors of AMPK (compound C) and eNOS (L-NAME) wiped off RA's beneficial effects. All these results demonstrated that RA attenuated the endothelial dysfunction induced by oxidative stress by activating the AMPK/eNOS pathway.

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Section: Introductionmentioning

confidence: 99%

Rosmarinic Acid Alleviates the Endothelial Dysfunction Induced by Hydrogen Peroxide in Rat Aortic Rings via Activation of AMPK

Zhou

et al. 2017

Oxidative Medicine and Cellular Longevity

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show abstract

“…In line with findings of this present study, findings of past studies showed both high and low salt diets displayed pro-inflammatory activities [ 51 , 52 ]. In some other studies, high and low salt diets were also found to enhance COX-2 expression [ 53 , 54 ] and IL-6 concentration [ 52 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Common salt aggravated pathology of testosterone-induced benign prostatic hyperplasia in adult male Wistar rat

Bello,

Omigbodun,

Morhason-Bello

2023

BMC Urol

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Background Benign prostatic hyperplasia (BPH) is a major health concern associated with lower urinary tract symptoms and sexual dysfunction in men. Recurrent inflammation, decreased apoptotic rate and oxidative stress are some of the theories that explain the pathophysiology of BPH. Common salt, a food additive, is known to cause systemic inflammation and redox imbalance, and may serve as a potential risk factor for BPH development or progression. This study examined the effect of common salt intake on the pathology of testosterone-induced BPH. Methods Forty male Wistar rats were randomly divided into four equal groups of 10: a control and three salt diet groups-low-salt diet (LSD), standard-salt diet (SSD) and high-salt diet (HSD). The rats were castrated, allowed to recuperate and placed on salt-free diet (control), 0.25% salt diet (LSD), 0.5% salt diet (SSD) and 1.25% salt diet (HSD) for 60 days ad libitum. On day 33, BPH was induced in all the rats with daily injections of testosterone propionate-Testost® (3 mg/kg body weight) for 28 days. The rats had overnight fast (12 h) on day 60 and were euthanized the following day in order to collect blood and prostate samples for biochemical, molecular and immunohistochemistry (IHC) analyses. Mean ± SD values were calculated for each group and compared for significant difference with ANOVA followed by post hoc test (Tukey HSD) at p < 0.05. Results This study recorded a substantially higher level of IL-6, IL-8 and COX-2 in salt diet groups and moderate IHC staining of COX-2 in HSD group. The prostatic level of IL-17, IL-1β, PGE2, relative prostate weight and serum PSA levels were not statistically different. The concentrations of IGF-1, TGF-β were similar in all the groups but there were multiple fold increase in Bcl-2 expression in salt diet groups-LSD (13.2), SSD (9.5) and HSD (7.9) and multiple fold decrease in VEGF expression in LSD (-6.3), SSD (-5.1) and HSD (-14.1) compared to control. Activity of superoxide dismutase (SOD) and concentration of nitric oxide rose in LSD and SSD groups, and SSD and HSD groups respectively. Activities of glutathione peroxidase and catalase, and concentration of NADPH and hydrogen peroxide were not significantly different. IHC showed positive immunostaining for iNOS expression in all the groups while histopathology revealed moderate to severe prostatic hyperplasia in salt diet groups. Conclusions These findings suggest that low, standard and high salt diets aggravated the pathology of testosterone-induced BPH in Wistar rats by promoting inflammation, oxidative stress, while suppressing apoptosis and angiogenesis.

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A low-salt diet with candesartan administration is associated with acute kidney injury in nephritis by increasing nitric oxide

Yu¹,

Wang²,

Ren³

et al. 2023

Biomedicine & Pharmacotherapy

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Low-salt diet increases NO bioavailability and COX-2 vasoconstrictor prostanoid production in spontaneously hypertensive rats

Cited by 7 publications

References 41 publications

Rosmarinic Acid Alleviates the Endothelial Dysfunction Induced by Hydrogen Peroxide in Rat Aortic Rings via Activation of AMPK

Rosmarinic Acid Alleviates the Endothelial Dysfunction Induced by Hydrogen Peroxide in Rat Aortic Rings via Activation of AMPK

Common salt aggravated pathology of testosterone-induced benign prostatic hyperplasia in adult male Wistar rat

A low-salt diet with candesartan administration is associated with acute kidney injury in nephritis by increasing nitric oxide

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