Low-risk human papillomavirus type 6 DNA load and integration in cervical samples from women with squamous intraepithelial lesions

Coutlée, François; Trottier, Helen; Gagnon, Simon; Koushik, Anita; Richardson, Harriet; Roger, Michel; Ferenczy, Alex; Franco, Eduardo L.

doi:10.1016/j.jcv.2009.03.019

Cited by 8 publications

(10 citation statements)

References 12 publications

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“…Samples were tested with an internal control to assess the presence of inhibition of the amplification reaction as described previously [Lefevre et al, ]. All samples were free of inhibitors and were tested in duplicate to measure the cellular content of samples by quantitation of β‐globin DNA using standard protocol [Khouadri et al, ; Coutlee et al, ]. The quantity of HPV6 contained in samples was measured in duplicate with a protocol published previously.…”

Section: Methodsmentioning

confidence: 99%

Juvenile respiratory papillomatosis: Risk factors for severity

Rodier

Lapointe

Coutlée

et al. 2013

Journal of Medical Virology

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Juvenile recurrent respiratory papillomatosis is caused mainly by human papillomavirus genotypes 6 or 11, acquired at birth or during pregnancy from an infected mother. Recurrent respiratory papillomatosis is characterized by recurring warts growing most commonly in the larynx. Multiple surgical procedures and the risk of airway obstruction contribute to the devastating impact of this disease. Some children will go into remission after a few surgeries whereas others will require repeated interventions over several years. Further understanding of the risk factors associated with severity may contribute to tailored treatments. A retrospective study of cases diagnosed between January 1995 and December 2008 was conducted to study determinants of severe forms of juvenile recurrent respiratory papillomatosis. Demographic and clinical variables were abstracted from children's medical charts and mothers' delivery charts. Viral factors (HPV genotyping and viral load) were studied from archived biopsies. Specific HLA class II alleles and killer-cell immunoglobulin-like receptors genes were tested from saliva samples. Logistic regression was performed to identify risk factors for severity. Overall, 31 pediatric cases of recurrent respiratory papillomatosis were identified. The only significant factor associated with severe forms of recurrent respiratory papillomatosis was the maternal history of condylomas during pregnancy (OR: 12.05 [P=0.05]). The analysis failed to identify risk factors that could be used clinically to identify recurrent respiratory papillomatosis cases likely to take a severe course. Although too early to determine, vaccination against the HPV types involved most commonly in recurrent respiratory papillomatosis may provide the best hope to prevent severe forms of this disease.

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Section: Methodsmentioning

confidence: 99%

Juvenile respiratory papillomatosis: Risk factors for severity

Rodier

Lapointe

Coutlée

et al. 2013

Journal of Medical Virology

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“…HPV is confirmed to be involved in the etiology of uterine cervix lesions. Recently, Coutlée et al . reported that samples from women with low grade squamous intraepithelial lesions (LSIL) contained higher HPV‐6 loads than women without this lesion. 1 Despite the existence of an anti‐HPV vaccine, HPV gene expression is dramatically altered during cervical carcinogenesis and novel biomarkers are needed for a better characterization of neoplastic cervical progression 2 .…”

mentioning

confidence: 99%

“…Recently, Coutlée et al reported that samples from women with low grade squamous intraepithelial lesions (LSIL) contained higher HPV-6 loads than women without this lesion. 1 Despite the existence of an anti-HPV vaccine, HPV gene expression is dramatically altered during cervical carcinogenesis and novel biomarkers are needed for a better characterization of neoplastic cervical progression. 2 The presence of HPV is likely, but not necessary, for the development of precursors and neoplastic lesions of the uterine cervix.…”

mentioning

confidence: 99%

Detection of early lymphangiogenesis by lymphatic microvascular density and endothelial proliferation status in preneoplastic and neoplastic lesions of the uterine cervix

Cimpean

Mazuru

Cernîi³

et al. 2011

Pathology International

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The characteristics of lymphangiogenesis in preneoplastic and neoplastic lesions of the uterine cervix are not well known and the role of this process in tumor progression and metastasis is not well understood. The aim of the present study was to characterize the morphology and distribution of lymphatic vessels and lymphatic proliferative status and to evaluate the value of lymphatic microvascular density (LMVD) in premalignant and malignant lesions of the uterine cervix. One hundred and twenty-eight paraffin-embedded cervical specimens were immunostained with D2-40 antibody specific for lymphatic endothelial cells. Colocalization of D2-40 and Ki67 for the proliferative characterization of lymphatic vessels was obtained by performing double immunostaining. A low density of lymphatic vessels was detected in normal cervix and squamous metaplasia. Intense and particular lymphangiogenic response was found in low and high grade squamous intraepithelial lesions and microinvasive carcinoma. Lymphatic proliferation occurred early in cervical lesions, being more active in premalignant lesions and microinvasive carcinomas than in invasive lesions. Our results suggest an early initiation of an active lymphangiogenesis in cervical lesions. These findings support the hypothesis that cervical preneoplastic lesions represent a critical point in the development of the lymphatic network vasculature. Early lymphangiogenesis could explain lymph node metastasis associated with cervical invasive carcinomas at preliminary diagnosis.

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“…The detection of the low‐risk HPV 6 in a squamous carcinoma is interesting. HPV 6 is generally associated with condylomata and does not integrate into the human genome 37 . Thus, its presence is more likely to be a co‐infection with a carcinogenic HPV not separately typed in this study.…”

Section: Discussionmentioning

confidence: 82%