Low-Protein Diet Induces Oral Tolerance to Ovalbumin in Mice.

Satter, Mohammed A.; Sakai, Kentaro; Ahmed, Sherin; Yoshino, Kenji; Yamamoto, Shigeru; Shimizu, Yūji; Ota, Fusao

doi:10.3177/jnsv.48.51

Cited by 11 publications

(13 citation statements)

References 36 publications

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“…The dose and nature of antigen, and strain and age of animals are among the most influential ones. The protocol used for the induction of oral tolerance in the current study is identical with that used in previous publications from our laboratory showing antigen specificity for the immune responses including OVA-specific IgE antibody (15,16). Judging from levels of OVA-specific IgE, mice fed with the NP diet were relatively resistant to induction of the oral tolerance to OVA which agrees with our previous report (15).…”

Section: Discussionsupporting

confidence: 80%

“…Among allergic reactions, type I hypersensitivity (IgE mediated allergic reaction) is one of the most prevalent types of food allergies (10). It has been reported previously from our laboratory that mice fed with a low protein diet were more prone to the induction of oral tolerance to OVA (15). Using ␤-lactoglobulin, a major allergen in cow's milk, it was latter confirmed that this effect of protein on the induction of tolerance was not limited to OVA (16).…”

Section: Discussionsupporting

confidence: 57%

“…IgE antibody levels against OVA were determined by an indirect ELISA method as described previously ( 15 ). Briefly, OVA was dissolved at a concentration of 100 g/mL in 50 m M carbonate buffer, pH 9.6, and 100 L of this was applied to each well of plates (Greiner Labortechnik, Gmbh).…”

Section: Methodsmentioning

confidence: 99%

“…Total IgE was examined by an ELISA method as previously described ( 15 ). Briefly microtiter plates (Greiner Labortechnik, Gmbh) were coated with rat antimouse IgE monoclonal antibody (Yamasa Corporation, Chiba, Japan) diluted in carbonate buffer (50 m M , pH 9.6) and then incubated overnight at 4˚C.…”

Section: Methodsmentioning

confidence: 99%

“…There are only a few reports focusing on the effect of nutritional status on oral tolerance to food antigens ( 14 ). We have previously reported the effect of protein restriction on oral tolerance ( 15,16 ). Although there is a report on the effect of moderate (10%) and high (40%) protein diets on IgE response in animals without induction of tolerance ( 17 ), there are no reports addressing the effect of high dietary protein on the induction of oral tolerance.…”

mentioning

confidence: 95%

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Modulation of Oral Tolerance to Ovalbumin by Dietary Protein in Mice

Alizadeh

Ota

Kassu

et al. 2006

J Nutr Sci Vitaminol

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SummaryThis study sought to determine whether oral tolerance to ovalbumin (OVA), responsible for food allergy, is affected by different amounts of protein intake. For this, 6-wkold BALB/c mice were fed with low protein (5%, LP), normal protein (20%, NP) and high protein (40%, HP) diets, orally given either OVA (OVA-fed) or water (Water-fed) for 4 d, and then immunized intraperitoneally twice at a 3-wk interval with alum-precipitated OVA. After the last immunization, sera were collected to measure total and OVA-specific IgE by enzyme assay (ELISA). Splenocytes were cultured and stimulated with concanavalin A (Con A), lipopolysaccharide (LPS) or OVA and assayed for 3 H-thymidine incorporation. The culture supernatants from their splenocytes stimulated with OVA were analyzed for interleukin (IL)-4, interferon (IFN)-␥ , and IL-12. Total IgE was significantly higher in OVA-fed HP groups as compared to NP and LP groups ( p Ͻ 0.05). The highest and the lowest OVA-specific IgE were observed in HP and LP diet groups, respectively ( p Ͻ 0.05). OVA-fed mice receiving the LP diet demonstrated significantly lower IL-4 as compared to the other two groups ( p Ͻ 0.05), while IFN-␥ was significantly higher in the LP compared to the HP group ( p Ͻ 0.05). Levels of IL-12 did not differ among the OVA-fed groups. Splenocytes of OVA-fed mice kept on the LP and HP diet showed significant impairment of proliferation to OVA as compared to the NP group ( p Ͻ 0.01). Proliferation against Con A was impaired in the LP group compared to the NP group ( p Ͻ 0.05) but not in Water-fed groups. However, it was higher against LPS in the HP than the LP group ( p Ͻ 0.05) both in Water-fed and OVA-fed animals. All these findings indicate that established oral tolerance to OVA is clearly affected by the amount of protein diet. They support the suggestion that dietary protein plays an important role(s) in IgE-mediated food allergies.

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Section: Discussionsupporting

confidence: 80%