Low prevalence of occult hepatitis B virus infection in chronic haemodialysis and kidney transplant patients

Muche, Marion; Berg, Thomas; Rimpler, Sunda; Staedtler, Adrienne; Böhm, Stefan; Nickel, Peter; Baid-Agrawal, Seema

doi:10.1111/liv.13951

Cited by 9 publications

(9 citation statements)

References 40 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study by Ramezani et al (15), HBV-DNA was detected in 1% of HBsAg negative patients. Similar to that study, Muche et al (16) found nil prevalence of OHB in chronic hemodialysis patients and a very low prevalence (<1%) in renal transplant patients suggesting that routine screening for HBV-DNA was not required in chronic hemodialysis population in their region.…”

Section: Discussionsupporting

confidence: 68%

Occult Hepatitis B in Hemodialysis Patients

Adar¹,

Köse²,

Tatar³

2019

vhd

View full text Add to dashboard Cite

Amaç: Risk grubunda yer alan hemodiyaliz hastalarında okült hepatit B'yi (OHB) saptamayı amaçladık. Gereç ve Yöntemler: Mayıs 2013-Temmuz 2013 arasında, İzmir ilinde 10 farklı diyaliz merkezinde hemodiyalize giren, 18 yaş ve üzeri, 567 kronik böbrek yetmezliği olan hastalar çalışmaya dahil edildi. Hepatit B yüzey antijeni (HBsAg), anti-hepatit B çekirdeği (HBc) immünoglobulin (IgG) ve anti-HBs ELISA yöntemiyle, HBV-DNA ise polimeraz zincirleme reaksiyonu (PCR) yöntemiyle çalışıldı. HbsAg negatifliği ve HBV-DNA pozitifliği OHB olarak değerlendirildi. Bulgular: Beş yüz altmış yedi hastanın %49'u erkek, ortalama yaş 62,2 idi. HBsAg tüm hastalarda negatif saptandı. Izole anti-HBc IgG pozitifliği 8 hastada gözlenmiş olup hiçbirinde HBV-DNA saptanamadı. Sadece bir hastada (%0,2) serum HBV-DNA düzeyi 270 IU/mL olarak ölçüldü. Sonuç: HBsAg tek başına HBV enfeksiyonunu saptamada yeterli bir serolojik test değildir. OHB şüphesi olan hastalarda HBV-DNA düzeyi moleküler tanı yöntemleriyle test edilmelidir. Maliyet etkinliği ve tanıda PCR'nin rolü için daha fazla çalışmalara ihtiyaç olduğu kanaatine varıldı. Anahtar Kelimeler: Kronik viral hepatit, hemodiyaliz, okült hepatit B ÖZ Objectives: We aimed to detect occult hepatitis B (OHB) in hemodialysis patients at a higher-risk for OHB. Materials and Methods: The study included 567 patients with chronic renal failure aged 18 years and older who underwent hemodialysis in 10 dialysis centers in İzmir province between May 2013 and July 2013. Hepatitis B surface-antigen (HBsAg), antihepatitis B core (HBc) immunoglobulin G (IgG) and anti-HBs were detected by ELISA and HBV-DNA levels with polymerase chain reaction (PCR). Detection of HBsAg negativity with HBV-DNA positivity was considered as OHB. Results: Of 567 patients, 49% were male and the mean age was 62.2 years. All the patients were HBsAg-negative. Isolated anti-HBc IgG positivity was detected in 8 patients while HBV-DNA was negative. Serum HBV-DNA level was 270 IU/mL in only one patient (0.2%) who was anti-HBc IgG-negative. Conclusion: HBsAg alone is not an adequate serological test to detect HBV infection. HBV-DNA should be tested using molecular diagnostic methods in patients with suspected OHB. Further studies investigating cost-effectiveness and the role of PCR in diagnosis are warranted.

show abstract

Section: Discussionsupporting

confidence: 68%

Occult Hepatitis B in Hemodialysis Patients

Adar¹,

Köse²,

Tatar³

2019

vhd

View full text Add to dashboard Cite

show abstract

“…This agrees with a study had carried out by (Mustafa et al, 2019) where they found OBI occurred in 38.1% of renal patients. However, it was much higher than studies have been undertaken in Brazil (Franz et al, 2013) and Germany (Muche et al, 2019).…”

Section: Discussionmentioning

confidence: 77%

Molecular Detection of Occult Hepatitis B virus in plasma and urine of renal transplant patients in Khartoum state Sudan

Ibrahim

Mohamed

Kambal

et al. 2020

International Journal of Infectious Diseases

View full text Add to dashboard Cite

The aim of this study was to determine the OBI in plasma and urine samples from renal transplant patients using Multiplex Nested PCR. Method: A total of 100 samples (plasma and urine) were collected from renal transplant patients admitted to the renal transplant center in Khartoum north, Sudan in 2019. For each sample, HBsAg, HBeAg and anti HBcAg were detected using Enzyme linked Immune sorbent assay (ELISA). The viral DNA was then extracted using viral DNA extraction kit and were then tested for HBV DNA by using multiplex nested PCR. Statistical analysis was done using statistical package of social science (IBM SPSS version 20.0) considering a P value 0.05 as a level of significance. Results: HBsAg were not detected in al patient but, HBeAg were 14 (14%) and anti HBcAg were 36 (36%) were detect by using ELISA. A total 18 (18%) and 3 out of 100 were found positive in plasma and urine samples, respectively. Regarding the virus genotypes, D, E and mixed D/E genotypes were detected in all positive samples. Females were significantly (P value = 0.013) higher detectable with HBV than males in plasma samples Conclusion: OBI incidence in renal transplant patients is high in Sudan. The multiplex nested PCR had identified OBI with a high rate supporting the efficiency of using molecular techniques in detecting of HBV. This will lead to an appropriate diagnosis and minimizing the risk to be infected by HBV.

show abstract

“…[21][22][23] The prevalence of occult HBV infection has been studied in different parts of the world and among various groups; however, there are conflicting with results even in a similar group. As an illustration, OBI prevalence among hemodialysis patients was 1.5% (Turkey), 24 0.5% (Iran), 25 0% (Germany), 26 1.3% (Japan), 27 and 0% (Italy) 28 ; in hemophilia patients was 1.73% in Pakistan, 29 9.3% in Iran, 30 and 0% in Polish 31 ; among thalassemia patients was 31.4% (India), 6 32.5% (Egypt), 5 and 0% (Iran) 13,14 ; in individuals with HCC was 30% (Japan), 32 38.5% (Korea), 33 50% (Egypt), 34 and 70.4% (China) 35 ; among blood donors in different parts of the world was 3.7% (Egypt), 36 4.71% (India), 37 1.98% (Colombia), 38 0.3% (Lebanon), 39 0% (Iran), 8 and 7.4% (Libya). 40 OBI also has been found in patients with leprosy in Brazil (5.3%), 41 individuals suffering from hyperlipidemia in China (9.5%), 42 patients with primary glomerulopathy (3%) and lupus nephritis (13.3%) in Iran, 43 and among ART (antiretroviral therapy) naïve HIV-seropositive individuals in Mozambique (8.3%).…”

Section: Resultsmentioning

confidence: 97%

Occult Hepatitis B Virus Infection among β-Thalassemia Major Patients in Ahvaz City, Iran

Amirhashchi

Azaran

Seyedian

et al. 2022

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Occult Hepatitis B Infection (OBI) is a critical risk factor for triggering post-transfusion hepatitis (PTH), cirrhosis, hepatocellular carcinoma, and hepatitis B virus (HBV) reactivation, which β-thalassemia major (BTM) patients are at risk of it due to multiple blood transfusions. This study was aimed at determining the prevalence of OBI among BTM patients from Khuzestan Province, Iran. In this cross-sectional study, 90 thalassemia patients, who have received blood 36 to 552 times, participated referred to the Shafa hospital of Ahvaz city from January 2018 to April 2019. ELISA for determining serological markers (HBsAg, anti-HBc, anti-HBs, and anti-HCV) and real-time PCR for detecting HBV-DNA were performed; Nested PCR was conducted for DNA sequencing and determining the genotype of OBI case. Phylogenetic and statistical analyses were done by R package. Of 90 subjects enrolled in this study; 95.5% (86/90) were HBsAg negative, and the frequency of OBI among them was 1.16% (1/86). The anti-HBs, anti-HBc, and anti-HCV were detected in 80.00%, 7.78%, and 12.2% of patients, respectively. HBV-DNA was assessed at four HBsAg-positive subjects as well, and all of them were negative. The phylogenetic analysis showed that the detected HBV DNA in the OBI case belongs to the genotype D. This research, for the first time, demonstrated that OBI is present among β-thalassemia patients in Iran. Also, further studies are necessary to determine the actual prevalence of OBI among BTM patients in Iran to decisions concerning OBI screening, especially in transfusion centers.

show abstract

Low prevalence of occult hepatitis B virus infection in chronic haemodialysis and kidney transplant patients

Cited by 9 publications

References 40 publications

Occult Hepatitis B in Hemodialysis Patients

Occult Hepatitis B in Hemodialysis Patients

Molecular Detection of Occult Hepatitis B virus in plasma and urine of renal transplant patients in Khartoum state Sudan

Occult Hepatitis B Virus Infection among β-Thalassemia Major Patients in Ahvaz City, Iran

Contact Info

Product

Resources

About