Low prevalence of HPV detection and genotyping in non-muscle invasive bladder cancer using single-step PCR followed by reverse line blot

Pichler, Renate; Borena, Wegene; Schäfer, Georg; Manzl, Claudia; Čulig, Zoran; List, S.; Neururer, Sabrina; Laer, Dorotheé von; Heidegger, Isabel; Klocker, Helmut; Horninger, Wolfgang; Steiner, Hannes; Brunner, Andrea

doi:10.1007/s00345-015-1539-y

Cited by 16 publications

(25 citation statements)

References 35 publications

(34 reference statements)

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“…Cyclophosphamide, chronic irritation from a stone and exposure to paints have a definite role in the development of bladder cancer (8-12). The role of HPV in bladder cancer is contradictory with publications supporting both its causative role in bladder cancer and others suggesting that HPV does not have any connection with bladder cancer (15, 16). The rarity of bladder cancer in young patients can be explained by the lack of hereditary bladder cancer syndromes, the fact that young patients are not usually exposed to common carcinogens, and of course in case of exposure to carcinogens (mainly smoking), the reported necessary interval of at least 20 years between the exposure and the development of bladder cancer cannot be satisfied to young patients and especially in our population of patients younger than 30 years, smoking had to be accompanied by one additional risk factor (2).…”

Section: Discussionmentioning

confidence: 63%

Bladder Cancer to Patients Younger than 30 Years: A Retrospective Study and Review of the Literature

et al. 2017

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Section: Discussionmentioning

confidence: 63%

Bladder Cancer to Patients Younger than 30 Years: A Retrospective Study and Review of the Literature

et al. 2017

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“…Mycobacterium bovis Bacillus Calmette-Guérin (BCG) is a live attenuated bovine tuberculosis vaccine that is the first choice for intravesical treatment, and it is the gold standard for the immunotherapy of superficial bladder cancer (3)(4)(5). Genitourinary tumours, such as bladder cancer (6) and benign diseases, including condyloma acuminatum and cervical genital warts, are closely related to human papillomavirus (HPV) infection (7)(8)(9). Clinical studies have established that BCG immunotherapy exerts a specific effect on genital warts of the genitourinary system (7,9).…”

Section: Introductionmentioning

confidence: 99%

BCG immunotherapy inhibits cancer progression by promoting the M1 macrophage differentiation of THP‑1 cells via the Rb/E2F1 pathway in cervical carcinoma

et al. 2021

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Bacillus Calmette-Guérin (BCG) immunotherapy increases macrophage polarization toward M1-type macrophages. In the present study, to identify the M1/M2 marker genes in the carcinogenesis and progression of cervical cancer, the microarray datasets GSE9750 and GSE7803 were downloaded from The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO) and the University of California Santa Cruz (UCSC) Xena browser. Survival analysis revealed that M1 markers (IL-12) were involved in anti-tumour progression, and M2 markers (IL-10) were involved in the carcinogenesis and invasion of cervical cancer. The expression of M1 markers (IL-12, inducible nitric oxide synthase and CD80) and M2 markers (IL-10 and arginase) was examined to determine whether BCG affects the polarization of macrophages and to elucidate the underlying mechanisms. The results revealed that BCG promoted macrophage polarization towards the M1 phenotype and enhanced the transition of M2 to M1 macrophages. The results also revealed that polarized M1 macrophages induced by BCG decreased the protein expression of phosphorylated (p-)retinoblastoma (Rb)/E2F transcription factor 1 (E2F1), inhibited the proliferation and promoted the apoptosis of HeLa cells. On the whole, these results demonstrated that BCG promoted the anti-tumour progression of M1 macrophages and inhibited the pro-tumour activation of M2 macrophages via the Rb/E2F1 signalling pathway in HeLa cells. This suggests the possibility of a direct translation of this combination strategy to clinical practice for the treatment of cervical cancer.

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“…Currently, there are several studies that described a correlation between HPV and development of bladder cancer. However, their found contradictory results [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Association of human papilloma virus (HPV) infection with oncological outcomes in urothelial bladder cancer

Moghadam

Mansori

Nowroozi

et al. 2020

Infect Agents Cancer

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Background: Bladder cancer is one of the leading causes of cancer death in adults worldwide. There are various risk factors described for the bladder cancer development including genetic background as well as environmental exposure. Currently, infectious agents such as human papilloma virus (HPV) has also been linked to bladder cancer risk. The current study aimed to evaluate the potential correlation between HPV infection and the oncological outcome in urothelial bladder cancer. Methods: Totally 106 tissue samples of histopathologically confirmed transitional cell carcinoma (TCC) of the urinary bladder were included in this study. The presence of high risk (types 16 and 18) and low risk (types 11 and 6) types of HPV was evaluated using polymerase chain reaction (PCR) followed by in situ hybridization. Results: Out of 106 bladder cancer patients, a total of 24 cases (22.6%) were positive HPV infection. The most common type of HPV detected was type 16 followed by types 11 and 18, and 6. According to independent T-test results, there was a significant association between mean age and HPV infection (P = 0.015). Moreover, our findings showed a significant relation between infection with HPV and tumor stage, tumor grade, muscle invasion of the tumor, as well as tumor recurrence. The results of Chi-square Test indicated that there is significant statistical association between types of HPV and tumor grade (P-Value = 0.044). Conclusion: Our findings indicated that a family history of cancer and HPV infection can be potential independent predictive factors for tumor recurrence in bladder cancer. Overall, the results of this study strongly indicate a significant relationship between HPV infection and an aggravated outcome of the disease and a higher risk of recurrence in patients with bladder cancer.

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Low prevalence of HPV detection and genotyping in non-muscle invasive bladder cancer using single-step PCR followed by reverse line blot

Abstract: Our study demonstrates low prevalence of HPV infection in FFPE bladder cancer tissue, arguing against the etiological role of HPV in non-muscle urothelial carcinogenesis.

Cited by 16 publications

References 35 publications

Bladder Cancer to Patients Younger than 30 Years: A Retrospective Study and Review of the Literature

Bladder Cancer to Patients Younger than 30 Years: A Retrospective Study and Review of the Literature

BCG immunotherapy inhibits cancer progression by promoting the M1 macrophage differentiation of THP‑1 cells via the Rb/E2F1 pathway in cervical carcinoma

Association of human papilloma virus (HPV) infection with oncological outcomes in urothelial bladder cancer

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