Low prevalence of circulating t(11;14)(q13;q32)–positive cells in the peripheral blood of healthy individuals as detected by real-time quantitative PCR

Incidence of preclinical manifestations of mantle cell lymphoma and mantle cell lymphoma in situ in reactive lymphoid tissues

“…4 This prevalence is a magnitude lower than that of circulating t(14;18)-positive cells, which were detected in 39 of 86 individuals (45%) in the same analysis. Correspondingly, in a recent study of the same cohort analyzed in this study, we had 'In situ' and preclinical manifestations of mantle cell lymphoma P Adam et al identified follicular lymphoma 'in situ' in 3 of the 132 (2%) patients without history of lymphoma, indicating that the higher frequency of t(14;18)-positive circulating cells is paralleled by a higher incidence of follicular lymphoma 'in situ' compared with mantle cell lymphoma 'in situ'.…”

“…1 Interestingly, rare B-cells carrying the t(11;14) translocation can also be found in the peripheral blood of healthy individuals. 2,3 Hirt et al 4 found circulating t(11;14)-positive cells in one of 100 healthy individuals at a frequency of 0.6 t(11;14) translocation copies/10 5 peripheral blood mononuclear cells. In contrast to this low prevalence, t(14;18)-positive B cells, ie, follicular lymphoma-like B cells were detected in 39 of 86 individuals (45%) in the same study.…”

t(11;14)-positive clones can persist over a long period of time in the peripheral blood of healthy individuals

“…In one individual, R101, a second clone appeared in the blood sample collected 7 years after enrolment. We show here that circulating t(11;14)-positive cells can be found in HI at a higher prevalence than described earlier, 7 and that their frequency can occasionally go beyond 10 -5 . Based on nucleotide sequence analysis, the translocations found in HI seem indistinguishable from those found in MCL.…”

“…To date, the presence of t(11;14)-positive cells in peripheral blood from HI has only been reported in one individual over the hundred HI studied and at a relatively low frequency (Bsix copies in 1 million normal cells). 7 In this paper, we used a sensitive fluctuation nested PCR analysis to determine the prevalence, frequency and long-term evolution of circulating t(11;14)-positive cells in the peripheral blood of 71 healthy males who had been examined earlier for the t(14;18) translocation using a similar approach. 2,3 Twenty eight individuals, who were not exposed to pesticides, were issued from a cohort of hospital and laboratory workers and were defined as reference population (mean age at enrolment: 41, min-max: 25-56).…”

The Wnt signaling pathway and mitotic regulators in the initiation and evolution of mantle cell lymphoma: Gene expression analysis

“…Second, what is required for the initial pathogenesis of MCL in addition to the t(11;14) translocation? A recent study has shown that the t(11;14) translocation alone is not sufficient to produce tumors (3) and, though at very low levels, t(11;14)-positive cells have been found in the blood of healthy individuals (4). Thus, key molecular behavior in MCL is thought to be divided into two major stages: initial lymphomagenesis in addition to the t(11;14) translocation (initiation) and the accumulation of variable secondary genomic alterations occurring over time, leading to the evolution into more aggressive forms (transformation).…”