Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy

Yu, Jiaxin; Sun, Xian-He; Cai, Fuhong; Zhu, Zhiliang; Qin, Anjun; Qian, Jun; Tang, Benzhong; He, Sailing

doi:10.1364/ol.40.002313

Cited by 39 publications

(23 citation statements)

References 19 publications

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“…Some of the key imaging and therapy applications of multifunctional AIEgens. Some elements are adapted with permission, Copyright 2015, American Chemical Society; and adapted with permission, Copyright 2016, Wiley‐VCH Verlag GmbH & Co. KGaA.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional AIEgens for Future Theranostics

Feng

Liu

2016

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The combination of diagnosis and therapeutics into one theranostics system has attracted great interest in life science and biomedical fields. The current theranostic platform largely relies on the integration of multiple materials with different functionalities. The all-in-one approach has the risk of high complicity with reduced reproducibility. Smart design of simple molecules born with multifunctions should represent one of the future directions in theranostics. Fluorogens with aggregation-induced emission (AIEgens) are one type of such smart materials, which have attracted increasing attentions in recent years. In this concept, the key frontier developments of simple AIEgens with multifunctions for imaging and therapy are presented, which include fluorescence-photoacoustic imaging, fluorescence-magnetic resonance imaging, fluorescence image-guided photodynamic therapy, fluorescence image-guided chemotherapy and photoacoustic image-guided photothermal therapy. The smart molecular design to endow each AIEgen with strong capability to simultaneously offer two or more theranostic functions should attract more scientists into this exciting research direction.

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Section: Introductionmentioning

confidence: 99%

Multifunctional AIEgens for Future Theranostics

Feng

Liu

2016

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134

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“…Coumarin 102 is a commonly used OSM in STED microscopy . However, it emits green fluorescence upon excitation, far below the “therapeutic window” (near‐infrared window is also known as optical window or therapeutic window, in the range of 650–1350 nm ).…”

Section: Category Two: Organic Small Molecules (Osms)mentioning

confidence: 99%

“…Yu et al. recently used AIE dots as fluorescent probes of STED . These AIE dots are brighter and more photo‐stable than coumarin 102 (an OSM probe).…”

Section: Category Four: Nanoparticles (Nps)mentioning

confidence: 99%

“…Fluorescent proteins (FPs), organic small molecules, (OSMs) and quantum dots (QDs) are three major types of fluorescent probes that are extensively used for nanoscopes . Recent emergence of nanoparticles (NPs), such as carbon dots (CDs), graphene quantum dots (GQDs), gold nanorods (AuNRs), polymer‐based nanoparticles (P‐dots), and aggregation‐induced emission dots (AIE dots), broadens beyond these three types . These four categories of probes constitute the “fluorescence toolbox” for nanoscopes.…”

Section: Introductionmentioning

confidence: 99%

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Fluorescent probes for nanoscopy: four categories and multiple possibilities

Zhuo

et al. 2016

Journal of Biophotonics

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Nanoscopy enables breaking down the light diffraction limit and reveals the nanostructures of objects being studied using light. In 2014, three scientists pioneered the development of nanoscopy and won the Nobel Prize in Chemistry. This recognized the achievement of the past twenty years in the field of nanoscopy. However, fluorescent probes used in the field of nanoscopy are still numbered. Here, we review the currently available four categories of probes and existing methods to improve the performance of probes.

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“…However, the cross‐section of the stimulated emission is gradually reduced as the wavelength moves away from the maximum of the emission spectrum, resulting in a huge demand for laser power to inhibit molecules in the excited state. High laser power is harmful to fluorescent probes and biological samples because of photobleaching and phototoxicity, which impedes the application of STED nanoscopy in biological research …”

Section: Introductionmentioning

confidence: 99%

Elimination of Re‐excitation in Stimulated Emission Depletion Nanoscopy Based on Photon Extraction in a Phasor Plot

Chen

Wang

Yan

et al. 2020

Laser & Photonics Reviews

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Stimulated emission depletion (STED) nanoscopy can achieve super‐resolution imaging of microstructures at the nanoscale by confining the fluorescence emission of a diffraction‐limited focusing spot. The depletion wavelength is typically at the red tail of the emission spectrum of the fluorophore to avoid re‐excitation; however, inefficient fluorescence inhibition on account of the small stimulated emission cross‐section results in a huge demand for the depletion of laser power. Here, an accurate photon extraction method is proposed to eliminate re‐excitation caused by blue shift of the depletion wavelength. After only extracting the photons unaffected by a donut‐shaped depletion laser in the phasor plot, both the resolution and signal‐to‐noise ratio of the STED images are significantly improved. As a result, the increased depletion efficiency at a blue shifted depletion wavelength reduces the power demand for achieving a certain resolution. This approach, with flexible wavelength selectivity owing to the independence of depletion power, made more fluorophores applicable for STED super‐resolution imaging, thereby offering more convenient and efficient service to researchers.

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Low photobleaching and high emission depletion efficiency: the potential of AIE luminogen as fluorescent probe for STED microscopy

Cited by 39 publications

References 19 publications

Multifunctional AIEgens for Future Theranostics

Multifunctional AIEgens for Future Theranostics

Fluorescent probes for nanoscopy: four categories and multiple possibilities

Elimination of Re‐excitation in Stimulated Emission Depletion Nanoscopy Based on Photon Extraction in a Phasor Plot

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