Low oxygen concentrations for embryo culture in assisted reproductive technologies

Bontekoe, Stephan; Mantikou, Eleni; Wely, Madelon van; Seshadri, Srividya; Repping, Sjoerd; Mastenbroek, Sebastiaan

doi:10.1002/14651858.cd008950

Cited by 3 publications

(3 citation statements)

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“…This is consistent with the evidence that cumulus cells can protect oocytes from micro-environment modification or sub-optimal culture conditions. However, given current evidences [5,27], we do believe that incubators without oxygen control should be replaced if possible; if not, they can be probably used until cumulus cells removal without a relevant reduction in both quality and quantity of produced embryos.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, in humans, a beneficial effect of lowering atmospheric O 2 tension to 5 % has been observed both for embryo quality and pregnancy rates, mainly in trials in which embryos were transferred at the blastocyst stage [3][4][5]. Although ideal culture conditions have not been established yet, most laboratories are discarding old generation incubators in order to control O 2 concentration in the culture system.…”

Section: Introductionmentioning

confidence: 99%

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Can we use incubators with atmospheric oxygen tension in the first phase of in vitro fertilization? A retrospective analysis

Guarneri

Restelli

Mangiarini

et al. 2014

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Can we use incubators with atmospheric oxygen tension in the first phase of in vitro fertilization? A retrospective analysis

Guarneri

Restelli

Mangiarini

et al. 2014

J Assist Reprod Genet

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“…Since the inception of clinical in vitro fertilization (IVF), assisted reproductive technology (ART) laboratories have focused on improving embryo culture systems. There have been significant developments in ART procedures over the past two decades, including the use of new culture medium (Gardner & Lane, 1997;Mantikou et al, 2013b;Summers & Biggers, 2003), the change from a 2 to 3 day culture duration to a 5-6 day culture duration (Marek et al, 1999;Papanikolaou et al, 2006), and the application of hypoxic medium that reportedly increases embryonic development and clinical outcomes (Bontekoe et al, 2012;Mantikou et al, 2013a). Additionally, there has been a switch from static to dynamic culture systems, including tilting embryo culture, microfluidic culture, and mechanical vibration during culture (Kim et al, 2009; the Ministry of Science and Technology (MOST) for the review and approval of human genetic resources.…”

Section: Introductionmentioning

confidence: 99%

Comparing transcriptome profiles of human embryo cultured in closed and standard incubators

Huang

Han

et al. 2020

PeerJ

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It is necessary to compare the transcriptomic profiles of human embryos cultured in time-lapse imaging (TLI) incubators and standard incubators (SI) in order to determine whether a closed culture system has a positive impact on embryos. In this study, we used RNA-sequencing (RNA-Seq) to characterize and compare the gene expression profiles of eight-cell embryos of the same quality grade cultured in TLI and SI. We sequenced a total of 580,952,620 reads for zygotes, TLI-cultured, and SI-cultured eight-cell embryos. The global transcriptomic profiles of the TLI embryos were similar to those of the SI embryos and were highly distinct from the zygotes. We also detected 539 genes showing differential expression between the TLI and SI groups with a false discovery rate (FDR) < 0.05. Using gene ontology enrichment analysis, we found that the highly expressed SI genes tended to execute functions such as transcription, RNA splicing, and DNA repair, and that the highly expressed TLI genes were enriched in the cell differentiation and methyltransferase activity pathways. This study, the first to use transcriptome analysis to compare SI and TLI, will serve as a basis for assessing the safety of TLI application in assisted reproductive technology.

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Culture Systems: Low-Oxygen Culture

Kovačič

2012

Methods in Molecular Biology

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The tension of oxygen measured in the oviducts of several mammals was 5-8.7 %, but this drops in the uterine milieu to <2 % in cows and primates. For embryo culture in human in vitro fertilization (IVF), a non-physiologic level of 20 % oxygen has been used for the past 30 years. However, several animal studies have shown that low levels of oxygen plays an important physiological role in reducing the high levels of detrimental reactive oxygen species within cells, influences the embryonic gene expression, helps with embryo metabolism of glucose, and enhances embryo development to blastocysts. However, clinical studies have given contradictory results. Nevertheless, in nearly all reports, some kind of improvement has been observed, either in embryo development or in implantation and no detriments have been reported. For these reasons, more and more IVF laboratories utilize low oxygen during embryo culture.

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Low oxygen concentrations for embryo culture in assisted reproductive technologies

Cited by 3 publications

References 11 publications

Can we use incubators with atmospheric oxygen tension in the first phase of in vitro fertilization? A retrospective analysis

Can we use incubators with atmospheric oxygen tension in the first phase of in vitro fertilization? A retrospective analysis

Comparing transcriptome profiles of human embryo cultured in closed and standard incubators

Culture Systems: Low-Oxygen Culture

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