Low‐oxygen and high‐carbon‐dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia

Jeanne, Michel; Filipović, Milica Kovačević; Szyporta, Milène; Vlaški, Marija; Hermitte, Francis; Lafarge, Xavier; Duchez, Pascale; Boiron, Jean‐Michel; Perreten, Vincent; Ivanović, Zoran

doi:10.1111/j.1537-2995.2009.02191.x

Cited by 14 publications

(16 citation statements)

References 26 publications

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“…In this study, we show that mammalian cells stored at 1°C or 5°C significantly reduced the viability and recovery although their viabilities are distinguished under the treatment of hypothermia (Figs 1E and 2B–2D). It has been shown that short-term storage of hematopoietic cells at 4°C substantially reduces the live cell number and function of progenitors after stored for 3 days [17], however, Louis et al showed that short-term processing at 4°C before cryopreservation can improve cell recovery compared with that at room temperature [18]. Our result is agreement with the former’s when mammalian cell lines were directly suspended their own culture medium and stored under severe hypothermia.…”

Section: Discussionsupporting

confidence: 89%

“…In this study the leibovitz’s L15 medium was used to store M2 and Hela cells under hypothermia, but no improvement had been observed for the viability. Previous work showed that the ratio of oxygen and carbon dioxide within storage container can affect cell viability when blood cells were stored at 4°C [17, 21, 22]; however, how the ratio of oxygen and carbon dioxide affect the viability of mammalian cell lines within our tested system remains to be investigated in the future.…”

Section: Discussionmentioning

confidence: 99%

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The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

et al. 2017

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Mammalian cells are very important experimental materials and widely used in biological and medical research fields. It is often required that mammalian cells are transported from one laboratory to another to meet with various researches. Conventional methods for cell shipment are laborious and costive despite of maintaining high viability. In this study we aimed to develop a simple and low-cost method for cell shipment by investigating the viabilities of different cell lines treated at different temperatures. We show that the viability of mammalian cells incubated at 1°C or 5°C significantly reduced when compared with that at 16°C or 22°C. Colony formation assays revealed that preservation of mammalian cells at 1°C or 5°C led to a poorer recovery than that at 16°C or 22°C. The data from proliferation and apoptotic assays confirmed that M2 cells could continue to proliferate at 16°C or 22°C, but massive death was caused by apoptosis at 1°C or 5°C. The morphology of mammalian cells treated under hypothermia showed little difference from that of the untreated cells. Quantitative RT-PCR and alkaline phosphatase staining confirmed that hypothermic treatment did not change the identity of mouse embryonic stem cells. A case study showed that mammalian cells directly suspended in culture medium were able to be shipped for long distance and maintained a high level of viability and recovery. Our findings not only broaden the understanding to the effect of hypothermia on the viability of mammalian cells, but also provide an alternative approach for cell shipment.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

et al. 2017

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“…INTRODUCTION Hamster and other mammalian hibernators offer the unique opportunity for studying what is nature's version of hematopoietic and other tissues preservation during cold exposure inducing state of controlled hypothermia (Carey et al, 2003). This "hamster model" is precious for many fields of application, from in vitro cell conservation (Jeanne et al, 2009) to research in space flight biology and medicine. With that respect, availability of a reliable in vitro system enabling the functional approach to the heterogenous progenitor cells population is essential.…”

Section: Hamster a Hibernating Animal Is An Important Experimental mentioning

confidence: 99%

Cultivation of hamster bone marrow haematopoietic stem and progenitor cells

Kovačević-Filipović¹,

Okic²,

Petricevic³

et al. 2010

Acta vet. (Beogr.)

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Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia

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“…For long term storage, temperatures in the range from -156ºC to -196ºC are recommeneded [46]. For short term storage not longer than 7 to 10 days, low oxygen and high carbon-dioxide atmosphere at 4ºC are suggested [47,48].…”

Section: Peripheral Blood Hematopoetic Stem and Progenitor Cells Are mentioning

confidence: 99%

The Blood is Rich in Different Types of Mesoderm Derived Stem and Progenitor Cells

Filipović

2014

Acta Veterinaria

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The blood and bone marrow have been thoroughly investiagated for more than a century, but we are still gaining surprising new informations. Blood transports different mature cells such as erythrocytes, platelets and granulocytes, but curiously, the blood is also transporting a number of non-differentiated cells of various mesodermal lineages: hematopoietic and mesenchymal stem and progenitor cells, endothelial progenitor cells and very small embryonal like cells are some of the most impressive examples. In adults the bone marrow is the source of practiclly all cells that could be found in the blood. Stem and progenitor cells egress from the bone marrow and home to the bone marrow or various tissues in a highly regulated manner. The fact that the hematopoetic stem and progenitor cells traffi c through the blood and repopulate the bone marrow niche is largely explored in stem cell therapy in human medicine. In this review we will briefl y describe the main characteristics of stem and progenitor cells, the mechanisms of their mobilization from the bone marrow and homing to target tissues. Also, the history and importance of the fact that different stem, progenitor and precursor cells could be isolated from the blood circulation will be discussed in the light of informations concerning their use in human and veterinary medicine.

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Low‐oxygen and high‐carbon‐dioxide atmosphere improves the conservation of hematopoietic progenitors in hypothermia

Abstract: Atmosphere with low O2 and high CO2 concentration (3% O2/6% CO2) in hypothermia (+4°C) during 7 days of storage prevents cell damage and preserves a high number of functional HSCs and progenitors mobilized in PB by granulocyte-colony-stimulating factor.

Cited by 14 publications

References 26 publications

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

The analysis of viability for mammalian cells treated at different temperatures and its application in cell shipment

Cultivation of hamster bone marrow haematopoietic stem and progenitor cells

The Blood is Rich in Different Types of Mesoderm Derived Stem and Progenitor Cells

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