Low occurrence of occult hepatitis B virus infection and high frequency of hepatitis C virus genotype 3 in hepatocellular carcinoma in Brazil

Alencar, Regiane S. S. M.; Gomes, Michele; Sitnik, Roberta; Pinho, J R R; Malta, Fernanda de Mello; Carvalho-Mello, Isabel Maria Vicente Guedes de; Mello, Evandro Sobroza de; Bacchella, Telésforo; Machado, Marcel Cerqueira César; Alves, Venâncio Avancini Ferreira; Carrilho, Flair José

doi:10.1590/s0100-879x2006005000197

Cited by 24 publications

(21 citation statements)

References 38 publications

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“…In two other investigations conducted in the State of São Paulo, OBI was diagnosed by nested PCR in sera of 12 of 50 (24%) (20) and 15 of 106 (14%) anti-HBc positive patients with chronic HCV infection (21). Differences in techniques, and the fact that all patients enrolled in these last two studies had markers of previous HBV infection, probably explain the differences in OBI prevalence in these investigations, our study, and that of Alencar et al (19). …”

Section: Discussionmentioning

confidence: 49%

“…A study of 50 patients with HCV-related liver cirrhosis with or without HCC who underwent liver transplantation in São Paulo, Brazil, and in which HBV DNA from serum and paraffin-embedded liver tissue was investigated using real time PCR, found only 1 case of OBI (19). Those authors ascribed this result to the low prevalence of HBV infection in the State of São Paulo.…”

Section: Discussionmentioning

confidence: 99%

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Occult hepatitis B virus infection in liver transplant patients in a Brazilian referral center

Ferrari

Xavier

Vidigal

et al. 2014

Braz J Med Biol Res

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Estimates of occult hepatitis B virus (HBV) infection prevalence varies among different studies depending on the prevalence of HBV infection in the study population and on the sensitivity of the assay used to detect HBV DNA. We investigated the prevalence of occult HBV infection in cirrhotic patients undergoing liver transplantation in a Brazilian referral center. Frozen liver samples from 68 adults were analyzed using a nested polymerase chain reaction assay for HBV DNA. The specificity of the amplified HBV sequences was confirmed by direct sequencing of the amplicons. The patient population comprised 49 (72.1%) males and 19 (27.9%) females with a median age of 53 years (range=18-67 years). Occult HBV infection was diagnosed in three (4.4%) patients. The etiologies of the underlying chronic liver disease in these cases were alcohol abuse, HBV infection, and cryptogenic cirrhosis. Two of the patients with cryptic HBV infection also presented hepatocellular carcinoma. Markers of previous HBV infection were available in two patients with occult HBV infection and were negative in both. In conclusion, using a sensitive nested polymerase chain reaction assay to detect HBV DNA in frozen liver tissue, we found a low prevalence of occult HBV infection in cirrhotic patients undergoing liver transplant, probably due to the low prevalence of HBV infection in our population.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Occult hepatitis B virus infection in liver transplant patients in a Brazilian referral center

Ferrari

Xavier

Vidigal

et al. 2014

Braz J Med Biol Res

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“…Patients were tested by PCR, and 17% were found to exhibit this profile. Alencar et al (2008) evaluated the presence of OBI in patients with HCV-related liver cirrhosis, with or without HCC, in São Paulo, and only one case with HCC had HBV DNA-positive serum.…”

Section: Discussionmentioning

confidence: 99%

Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil

Almeida

Mello

Menegoy

et al. 2017

Mem. Inst. Oswaldo Cruz

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BACKGROUND Many studies have identified mutations in the hepatitis B surface antigen (HBsAg) as important factors limiting the ability of commercial serological assays to detect this viral antigen. However, an association between mutations in the HBsAg gene and the occurrence of occult HBV infection (OBI) in patients has not been established.OBJECTIVES To detect hepatitis B virus (HBV) DNA in patients with anti-HBc as a unique serological marker, a previously published, cost-effective TaqMan-based real-time polymerase chain reaction (PCR) test with minor groove binding probes was adapted for use in this study. The current study also aimed to investigate HBsAg mutations and genotypes of HBV in OBI at the Viral Hepatitis Ambulatory Clinic in Rio de Janeiro to determine any possible association.METHODS Intra-assay and inter-assay reproducibility were determined, and the mean coefficient of variation values obtained were 2.07 and 3.5, respectively. Probit analysis indicated that the 95% detection level was 25 IU/mL. The prevalence of OBI was investigated in 35 serum samples with an ‘anti-HBc alone’ profile from individuals who attended our clinic between 2011 and 2013.FINDINGS HBV DNA was detected in only one sample, resulting in an OBI rate of 2.9%. Nucleotide sequencing of the pre-S/S region was performed to genotype and analyse mutations within the HBsAg gene of this HBV DNA. The HBV in the OBI case was classified as sub-genotype A1, and a sequence analysis of the small S gene revealed 12 mutations in the major hydrophilic region compared to the consensus A1 sequence. Most of these mutations occurred in amino acid residues that have been reported as clinically relevant because they have been implicated in vaccine escape and/or inability to detect HBsAg by commercial serological assays.MAIN CONCLUSIONS Our study suggests the importance of specific HBsAg mutations, different from those in D, B, and C genotypes, in sub-genotype A1 HBV associated with OBI.

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“…The prevalence of occult heaptitis B found in 15 different studies ranged from 1% to 95%, which might be accounted to differences between endemic and non endemic areas, presence of risk factors and to the sensitivity of the HBV-DNA assay [10]. On the other hand in some areas occult HBV infection has not been detected [11,12].…”

Section: Introductionmentioning

confidence: 97%

Anti-HBc and HBV-DNA detection in blood donors negative for hepatitis B virus surface antigen

Badrawy¹,

Bakry²

2013

AJMB

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Occult HBV infection is defined as the presence of hepatitis B virus (HBV) DNA in blood or liver tissues in patients negative for Hepatitis B surface Antigen (HBsAg). Those patients may or may not be positive for HBV antibodies. The objective of this study is to determine the presence or absence of HBV DNA in the serum samples from HBsAg negative blood donors. In addition we aimed to assess the magnitude of occult HBV infection and to reduce the risk of HBV infection. Over a period of one year a total of 7340 blood units were collected at blood transfusion center in our locality for the prevalence of HBV infection and 180 HBsAg negative blood specimens were randomly selected for anti-HBcIgM, anti-HBs antibody and HBV DNA. Ninety seven out of 7340 collected blood units were positive for HbsAg (1.3%). The randomly selected 180 tested donors revealed 7 (3.8%) positive for antiHBc IgM and 34 (18.8%) were positive for anti-HBs antibodies. Four out of 7 positive for anti-HBc IgM were also positive for anti-HBs and 2/180 (1.1%) specimens were positive for HBV DNA by PCR. Anti-HBc antibody should be tested routinely at any blood transfusion center and if they were positive regardless of anti-HBs titer, the blood should be discarded. Also HBV DNA is preferable to be performed to all blood donors to present completely safe blood transfusion.

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Low occurrence of occult hepatitis B virus infection and high frequency of hepatitis C virus genotype 3 in hepatocellular carcinoma in Brazil

Cited by 24 publications

References 38 publications

Occult hepatitis B virus infection in liver transplant patients in a Brazilian referral center

Occult hepatitis B virus infection in liver transplant patients in a Brazilian referral center

Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil

Anti-HBc and HBV-DNA detection in blood donors negative for hepatitis B virus surface antigen

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