Low molecular weight guluronate prevents TNF-α-induced oxidative damage and mitochondrial dysfunction in C2C12 skeletal muscle cells

Dun, Yunlou; Zhou, Xiaolin; Guan, Huashi; Yu, Guangli; Li, Chunxia; Hu, Ting; Zhao, Xia; Cheng, Xiaolei; He, Xiaoxi; Hao, Jiejie

doi:10.1039/c5fo00533g

Cited by 17 publications

(7 citation statements)

References 31 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Blood lactate and HR responses to WAnT were not altered following cupping treatment compared to the control. Our findings somewhat contrast the purported physiological mechanisms surrounding cupping therapy, including modulating metabolic acidosis, decreased oxidative stress, enhanced mitochondrial function, and increased oxygen availability and utilization (Arce-Esquivel, et al, 2020;Dun, et al, 2015;Ekrami, et al, 2021;Hofmann, et al, 2007;Powers & Jackson, 2008;Tagil, et al, 2014). Consequently, these were plausible mechanisms that could modify the physiological responses to the anaerobic exercise, to which the present study reports no beneficial effects based on HR and lactate responses.…”

Section: Discussioncontrasting

confidence: 94%

Influence of cupping treatment on high-intensity anaerobic performance

Al-Horani

Alsays

Ihsan

2022

Kinesiology (Zagreb, Online)

View full text Add to dashboard Cite

The use of cupping therapy prior to sports events has increased in popularity, with limited evidence to support its efficacy. The purpose of this study was to evaluate the efficacy of dry and wet cupping therapy on subsequent Wingate anaerobic test (WAnT) performance. Twelve trained men participated in this repeated-measures randomized crossover study (age 24.9 ± 4.8 years; body mass index 27.6 ± 14.3 kg.m-2). Participants were familiarized with the ergometer and the Wingate anaerobic test on three separate occasions. They then randomly performed three experimental Wingate tests separated by 48-72 h after either dry cupping (DRY), wet cupping (WET), or no treatment (CON). Repeated measures ANOVA and Pearson’s correlation coefficient were used to analyze data and determine the relationships between WAnT and peak lactate and heart rate (HR). Peak power (PP), mean power (MP), and fatigue index (FI) were similar in all treatments (p=.47-.72). Heart rate (HR) and lactate increased similarly at all time points in all treatments (p<.001 for all comparisons). Post-WAnT peak HR was moderately, negatively correlated with PP in all treatments and MP in CON only (p<.05 for all correlations). No other significant correlations were detected. The present findings demonstrate no beneficial effects of wet and dry cupping therapy, and hence do not support its use prior to high-intensity anaerobic sports events.

show abstract

Section: Discussioncontrasting

confidence: 94%

Influence of cupping treatment on high-intensity anaerobic performance

Al-Horani

Alsays

Ihsan

2022

Kinesiology (Zagreb, Online)

View full text Add to dashboard Cite

show abstract

“…Senescence and cellular metabolism of ECM are implicated in mitochondrial dysfunction . Exposure of cells to TNF‐α induces more generation of ROS, and overproduction of ROS can result in mitochondrial dysfunction . Defective mitochondria with lower mitochondrial membrane potential can produce much reactive oxygen species (ROS), which promotes the production of intracellular malondialdehyde (MDA) that is the cell membrane lipid peroxidation product.…”

Section: Discussionmentioning

confidence: 99%

“…37,38 Exposure of cells to TNF-α induces more generation of ROS, and overproduction of ROS can result in mitochondrial dysfunction. 39,40 Defective mitochondria with lower mitochondrial membrane potential can produce much reactive oxygen species (ROS), which promotes the production of intracellular malondialdehyde (MDA) that is the cell membrane lipid peroxidation product. In the current study, we found that PD alleviated the intracellular accumulation of ROS, improved the lowered mitochondrial membrane potential and rescued the impaired ATP production in NPCs treated with TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Polydatin suppresses nucleus pulposus cell senescence, promotes matrix homeostasis and attenuates intervertebral disc degeneration in rats

Wang

Huang

Lin

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Intervertebral disc degeneration (IVDD) is one of the major causes of low back pain. Polydatin (PD) has been shown to exert multiple pharmacological effects on different diseases; here, we test the therapeutic potential of PD for IVDD. In in‐vitro experiments, we confirmed PD is nontoxic to nucleus pulposus cells (NPCs) under the concentration of 400 μmol/L. Furthermore, PD was able to decrease the level of senescence in TNF‐α‐treated NPCs, as indicated by β‐gal staining as well as senescence markers p53 and p16 expression. In the aspect of extracellular matrix (ECM), PD not only reduced metalloproteinase 3 (MMP‐3), metalloproteinase 13 (MMP‐13) and a disintegrin‐like and metalloproteinase thrombospondin type 1 motif 4 (ADAMTS‐4) expression, but also increased aggrecan and collagen II levels. Mitochondrion is closely related to cellular senescence and ECM homeostasis; mechanistically, we found PD may rescue TNF‐α‐induced mitochondrial dysfunction, and it may also promote Nrf2 expression and activity. Silencing Nrf2 partly abolished the protective effects of PD on mitochondrial homeostasis, senescence and ECM homeostasis in TNF‐α‐treated NPCs. Correspondingly, PD ameliorated IVDD in rat model by promoting Nrf2 activity, preserving ECM and inhibiting senescence in nucleus pulposus cells. To sum up, our study suggests that PD exerts protective effects in NPCs against IVDD and reveals the underlying mechanism of PD on Nrf2 activation in NPCs.

show abstract

“…One previous study found that CS activity in slow‐twitch muscle is higher than that in the fast‐twitch muscle, 56 which is consistent with our findings. In addition, mitochondrial dysfunction through decreased SIRT1 expression is caused by increased levels of inflammatory cytokines generating higher levels of ROS 54,55,57 . Furthermore, ROS are produced as a by‐product of mitochondrial respiration 58 .…”

Section: Discussionmentioning

confidence: 99%

Differential effects of pre‐exercise on cancer cachexia‐induced muscle atrophy in fast‐ and slow‐twitch muscles

et al. 2020

View full text Add to dashboard Cite

We hypothesized that pre-exercise may effectively prevent cancer cachexia-induced muscle atrophy in both fast-and slow-twitch muscle types. Additionally, the fasttwitch muscle may be more affected by cancer cachexia than slow-twitch muscle. This study aimed to evaluate the effects of pre-exercise on cancer cachexia-induced atrophy and on atrophy in fast-and slow-twitch muscles. Twelve male Wistar rats were randomly divided into sedentary and exercise groups, and another 24 rats were randomly divided into control, pre-exercise, cancer cachexia induced by intraperitoneal injections of ascites hepatoma AH130 cells, and pre-exercise plus cancer cachexia groups. We analyzed changes in muscle mass and in gene and protein expression levels of major regulators and indicators of muscle protein degradation and synthesis pathways, angiogenic factors, and mitochondrial function in both the plantaris and soleus muscles. Pre-exercise inhibited muscle mass loss, rescued protein synthesis, prevented capillary regression, and suppressed hypoxia in the plantaris and soleus muscles. Pre-exercise inhibited mitochondrial dysfunction differently in fastand slow-twitch muscles. These results suggested that pre-exercise has the potential to inhibit cancer-cachexia-induced muscle atrophy in both fast-and slow-twitch muscles. Furthermore, the different progressions of cancer-cachexia-induced muscle atrophy in fast-and slow-twitch muscles are related to differences in mitochondrial function.

show abstract

Low molecular weight guluronate prevents TNF-α-induced oxidative damage and mitochondrial dysfunction in C2C12 skeletal muscle cells

Cited by 17 publications

References 31 publications

Influence of cupping treatment on high-intensity anaerobic performance

Influence of cupping treatment on high-intensity anaerobic performance

Polydatin suppresses nucleus pulposus cell senescence, promotes matrix homeostasis and attenuates intervertebral disc degeneration in rats

Differential effects of pre‐exercise on cancer cachexia‐induced muscle atrophy in fast‐ and slow‐twitch muscles

Contact Info

Product

Resources

About