Low‐molecular‐weight glutenin subunits from the 1U genome of <scp><i>Aegilops umbellulata</i></scp> confer superior dough rheological properties and improve breadmaking quality of bread wheat

Wang, Jian; Wang, Chang; Zhen, Shoumin; Li, Xiaohui; Yan, Yueming

doi:10.1002/jsfa.8700

Cited by 17 publications

(11 citation statements)

References 66 publications

(138 reference statements)

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“…In addition to the evolutionary contribution to polyploid Aegilops species, the diploid Aegilops species including those with U, M, C, and N genomes also harbor abundant beneficial genes for the genetic improvement of bread wheat with respect to, for instance, resistance to leaf and stripe rust (Sears, 1956;Riley et al, 1968;Riar et al, 2012;Toor et al, 2016;Bansal et al, 2017;Liu et al, 2019) and powdery mildew (Gill et al, 1985;Zhu et al, 2006;Weidner et al, 2012), tolerance to salt (Gorham, 1990) and aluminum stress (Miller et al, 1995), accumulation of zinc and iron (Wang et al, 2011;Neelam et al, 2012), high efficiency in zinc uptake (Cakmak et al, 1999), as well as high protein content and gluten content (Gong et al, 2017;Wang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping

et al. 2020

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Fluorescence in situ hybridization karyotypes have been widely used for evolutionary analysis on chromosome organization and genetic/genomic diversity in the wheat alliance (tribe Triticeae of Poaceae). The karyotpic diversity of Aegilops umbellulata, Ae. markgrafii, Ae. comosa subsp. comosa and subsp. subventricosa, and Ae. uniaristata was evaluated by the fluorescence in situ hybridization (FISH) probes oligo-pSc119.2 and pTa71 in combination with (AAC) 5 , (ACT) 7 , and (CTT) 12 , respectively. Abundant intra-and interspecific genetic variation was discovered in Ae. umbellulata, Ae. markgrafii, and Ae. comosa, but not Ae. uniaristata. Chromosome 7 of Ae. umbellulata had more variants (six variants) than the other six U chromosomes (2-3 variants) as revealed by probes oligo-pSc119.2 and (AAC) 5. Intraspecific variation in Ae. markgrafii and Ae. comosa was revealed by oligo-pSc119.2 in combination with (ACT) 7 and (CTT) 12 , respectively. At least five variants were found in every chromosome of Ae. markgrafii and Ae. comosa, and up to 18, 10, and 15 variants were identified for chromosomes 2 of Ae. markgrafii, 4 of Ae. comosa subsp. comosa, and 6 of Ae. comosa subsp. subventricosa. The six Ae. uniaristata accessions showed identical FISH signal patterns. A large number of intra-specific polymorphic FISH signals were observed between the homologous chromosomes of Ae. markgrafii and Ae. comosa, especially chromosomes 1, 2, 4, and 7 of Ae. markgrafii, chromosome 4 of Ae. comosa subsp. comosa, and chromosome 6 of Ae. comosa subsp. subventricosa. Twelve Ae. comosa and 24 Ae. markgrafii accessions showed heteromorphism between homologous chromosomes. Additionally, a translocation between the short arms of chromosomes 1 and 7 of Ae. comosa PI 551038 was identified. The FISH karyotypes can be used to clearly identify the chromosome variations of each chromosome in these Aegilops species and also provide valuable information for understanding the evolutionary relationships and structural genomic variation among Aegilops species.

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Section: Introductionmentioning

confidence: 99%

Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping

et al. 2020

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“…In Aegilops umbellulata , HMW-GS and LMW-GS genes are located on the long arm and short arm of chromosome 1U, respectively (Rawat et al . 2011, Wang et al . 2018).…”

Section: Resultsmentioning

confidence: 99%

A wheat-<i>Aegilops umbellulata</i> addition line improves wheat agronomic traits and processing quality

Jia

Yang

et al. 2019

Breed. Sci.

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Wheat processing quality is mainly correlated with high-molecular-weight glutenin subunits (HMW-GS) of grain endosperm. In bread wheat, the number of HMW-GS alleles are limited. However, wheat relative species possess numerous HMW-GS genes. In our previous study, a pair of novel HMW-GS 1Ux3.5+1Uy1.9 was characterized in Aegilops umbellulata. In this work, a novel wheat-Ae. umbellulata addition line, GN05, carrying a pair of 1U chromosome was developed and identified via cytogenetic analysis. Protein composition analysis indicated that GN05 carried HMW-GS of Ae. umbellulata. Accumulation of glutenin macropolymer (GMP) showed that GN05 had a much higher GMP content than the recurrent parent Chinese Spring. Rheological characteristics were analyzed by mixing test and the dough quality of GN05 was significantly improved compared to Chinese Spring. The results presented here may provide a valuable resource for the improvement of bread wheat quality.

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“…All the predicted α-helices in the Mr 42 K LMW-GS seem to be located near the intra-molecular disulfide bonds, suggesting that helix-helix interactions are involved in guiding the formation of the intra-molecular disulfide bonds (MASCI et al, 1998). Therefore, higher α-helix content may contribute to better quality of the dough (WANG et al, 2012a;WANG et al, 2018). In addition, the β-strands are generally considered to endow the protein with high elasticity and to improve the capability to resist distortion (SHEWRY et al, 2002).…”

Section: Secondary Structure Analysis Of Two Novel Lmw-m Glutenin Genesmentioning

confidence: 99%

Cloning and molecular characterization of two novel LMW-m type glutenin genes from Triticum spelta L.

et al. 2021

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Spelt wheat (Triticum spelta L., 2n=6x=42, AABBDD), as a hexaploid wheat species, is important sources of food and feed in Europe. It also serves as an important genetic resource for improvement of wheat quality and resistance. In this study, two novel m-type low molecularglutenin subunit (LMW-GS) genes, named as TsLMW-m1 and TsLMW-m2 were cloned by allelic specific polymerase chain reaction (AS-PCR)from German spelt wheat cultivars Rochbergers fruher Dinke and Schwabenkorn, respectively. The complete open reading frames (ORFs) of both genes contained 873 bp encoding 290 amino acid residues, and had typical LMW-GS structural features. Two same deletions with 24 bp at the position of 707-730 bp were present in both genes, while TsLMW-m1 had two nonsynonymous single-nucleotide polymorphism (SNP) variations at the positions of 434 bp (C-A transversion) and 857 bp (G-A transition). Phylogenic analysis revealed that both LMW-m genes were closely related to those from wheat A genome, suggesting that both subunits are encoded by the Glu-A3 locus. Secondary structure prediction showed that TsLMW-m1 and TsLMW-m2 subunits had more ?-helices than other wheat LMW-GS including superior quality subunit EU369717, which would benefit to form superior gluten structures and dough properties. The authenticity and expression activity of TsLMW-m1 and TsLMW-m2 genes were verified by prokaryotic expression in E. coli. Our results indicated that two newly cloned TsLMW-m genes could have potential values for wheat quality improvement.

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Low‐molecular‐weight glutenin subunits from the 1U genome of Aegilops umbellulata confer superior dough rheological properties and improve breadmaking quality of bread wheat

Abstract: Our results indicate that the 1U genome has superior LMW-GS that can be used as new gene resources for wheat gluten quality improvement. © 2017 Society of Chemical Industry.

Cited by 17 publications

References 66 publications

Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping

Analysis of Structural Genomic Diversity in Aegilops umbellulata, Ae. markgrafii, Ae. comosa, and Ae. uniaristata by Fluorescence In Situ Hybridization Karyotyping

A wheat-<i>Aegilops umbellulata</i> addition line improves wheat agronomic traits and processing quality

Cloning and molecular characterization of two novel LMW-m type glutenin genes from Triticum spelta L.

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