Low molecular weight components in bovine semen diffusate and their effects on motility of bull sperm

al-Somai, N; Vishwanath, R.; Shannon, Patrick; Molan, Peter C.

doi:10.1071/rd9940165

Cited by 19 publications

(14 citation statements)

References 20 publications

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“…The fraction of seminal plasma composed by low molecular weight proteins, that which are less than 10 kDa, was not beneficial to the sperm cells when added during cryopreservation, corroborating the observations that low molecular weight proteins reduce motility and cause damages in the membrane integrity of spermatozoa (AL-SOMAI et al, 1994a;AL-SOMAI et al, 1994b).…”

Section: Discussionsupporting

confidence: 67%

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Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

Fagundes

Tilburg²,

Souza³

et al. 2011

Acta Biomed.Bras.

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Difficulties in obtaining equine frozen semen with potential fertility are recognized. This study was designed to investigate the effect of seminal plasma on frozen/thawing of eight stallion semen from different breed using the following treatments: Seminal plasma with ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender; Part of seminal plasma with proteins under 10 kDa on frozen extender; Conventional freezing, using whole seminal plasma on frozen extender. Using the parameter of 30% of seminal motility post-thawing as index of good freezability, it was verified an increased percentage of stallions that presented good freezability when semen was frozen with seminal plasma containing ten-fold concentrated proteins with molecular weight above 10 kDa on frozen extender. These results, suggested the use of seminal plasma concentrated proteins from own stallion to freezing/thawing semen. ; van Tilburg, M.F.; Souza, G.V.; Caiado, J.R.C.; Barreto, M.A.P.; Silva, J.F.S. Effect of addition of concentrated proteins and seminal plasma low of addition of concentrated proteins and seminal plasma low molecular weight proteins in freezing and thawing of equine semen.

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Section: Discussionsupporting

confidence: 67%

“…Compounds containing proteins with low molecular weight induce damages on the spermatozoa, such as, the removal of those proteins of seminal plasma is important to survival and quality of spermatozoa (AL-SOMAI et al, 1994a;AL-SOMAI et al, 1994b).…”

Section: Introductionmentioning

confidence: 99%

Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

Fagundes

Tilburg²,

Souza³

et al. 2011

Acta Biomed.Bras.

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“…The contribution of the seminal vesicle secretions to sperm motility has been investigated using semen of several different mammals such as boar (Iwamoto et al 1992, Jeng et al 1993, Nichol et al 1997, bull (Al-Somai et al 1994), mouse (Peitz 1988) and human (Robert & Gagnon 1996). In mice, the removal of the seminal vesicle greatly reduces mouse sperm fertility (Pang et al 1979, Peitz & Olds-Clarke 1986.…”

Section: Introductionmentioning

confidence: 99%

The PATE gene is expressed in the accessory tissues of the human male genital tract and encodes a secreted sperm-associated protein

Soler-García

Maitra²,

Kumar³

et al. 2005

Reproduction

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ThePATEgene is expressed in prostate and testis. To determine if PATE is expressed in other accessory tissues of the male genital tract, RT-PCR of the epididymis and seminal vesicle was performed. PATE mRNA was highly expressed in the epididymis and seminal vesicle.In situhybridization of the testis showed PATE mRNA is strongly expressed in the spermatogonia. ThePATEgene encodes a 14-kDa protein with a predicted signal sequence and a cleavage site between residues G21 and S22. To determine if PATE is a secreted protein, 293T cells were transfected with a pcDNA-PATE-myc-His plasmid and protein immunoprecipitated with anti-myc monoclonal antibody. Western blot analysis showed the presence of PATE-myc-His protein was in the medium and the cell lysate. Confocal microscopy demonstrated that PATE-myc-His protein is found in the endoplasmic reticulum. The polyclonal antibody SOL-1 was generated by immunization of rabbits with recombinant PATE protein expressed and purified fromEscherichia coli.Western blots were performed on extracts of prostate, testis, seminal vesicle and ejaculated spermatozoa, but PATE protein was only detected in the spermatozoa. Immunostaining of sperm smears revealed that PATE is located in a band-like pattern in the sperm head. Our data indicate that PATE is made by various sexual accessory tissues and secreted into the semen where it becomes associated with sperm, suggesting that PATE is a novel sperm-associated protein with a possible role in mammalian sperm maturation.

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“…Honey is used as a traditional remedy for the treatment of peptic ulcers, gastritis and dyspepsia [20,129]. The discovery that Helicobacter pylori is a causative agent in many cases of peptic ulcers and dyspepsia raised the possibility that research on the antibacterial activity of honey may explain its therapeutic action.…”

Section: Use Of Honey In Peptic Ulcersmentioning

confidence: 99%

“…It is confirmed through earlier research that H. pylori was susceptible to honey at a concentration of 20% (v/v) and to manuka honey obtained from L. scobarium of New Zealand, with nonperoxide antibacterial activity, at a concentration of 5% (v/v), but none showed sensitivity to a 50% (v/v) solution of honey in which the antibacterial activity was primarily due to its hydrogen peroxide content. However, the clinical trials did not demonstrate the effective potency of manuka honey on H. pylori [74,129].…”

Section: Use Of Honey In Peptic Ulcersmentioning

confidence: 99%

Honey: Biological Characteristics and Potential Role in Disease Management

Shahid

2008

New Strategies Combating Bacterial Infection

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Honey is one of the oldest known medicines that has continued to be used in folk medicine and has been used as an adjuvant for accelerating wound healing. It is reported to prevent infection and promote healing since it has ingredients very similar to antibiotics. The major antibacterial properties of honey can be attributed to its low pH, high sugar content, enzymatic production of hydrogen peroxide, a thermolabile substance called inhibine and its hygroscopic properties. Several chemicals with antibacterial activity have also been identified [i.e. pinocembrin, terpenes, benzyl alcohol, 3,5-dimethoxy-4-hydroxybenzoic acid (syringic acid), methyl 3,5-dimethoxy-4-hydroxybenzoate (methyl syringate), 3,4,5-trimethoxybenzoic acid, 2-hydroxy-3-phenylpropionic acid, 2-hydroxybenzoic acid and 1,4-dihydroxybenzene]. However, the quantities of these substances are far too low to account for any significant amount of activity. Honey also serves as an important medicine because of its mild laxative, bactericidal, sedative and antiseptic characteristics. Recently, the emergence of multidrug-resistant (MDR) organisms has created a lot of chaos in the medical field. Hence, there is a need to find an alternative to counter these MDR organisms. This chapter will discuss the factors responsible for antimicrobial properties and potential use of honey as an antimicrobial agent. This chapter will also cover the effect of honey on MDR bacteria, discussing the few reports from available literature.

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Low molecular weight components in bovine semen diffusate and their effects on motility of bull sperm

Cited by 19 publications

References 20 publications

Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

Effect of Addition of Concentrated Proteins and Seminal Plasma Low Molecular Weight Proteins in Freezing and Thawing of Equine Semen

The PATE gene is expressed in the accessory tissues of the human male genital tract and encodes a secreted sperm-associated protein

Honey: Biological Characteristics and Potential Role in Disease Management

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