Low Molecular Weight Antibodies in Human Sera to Stratum corneum

Hk, Krogh

doi:10.1159/000230225

Cited by 8 publications

(9 citation statements)

References 6 publications

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“…The fractions obtained were then tested for adherence activity. Consistent with previous findings in adult sera [13,14], adherence activity was demonstrated in fractions containing low molecular weight yglobulins with a sedimentation rate less than albumin. The reactivity of these antibodies was unaffected by treatment with ME.…”

Section: Experiments and Resultssupporting

confidence: 92%

“…By double diffusion in agar a precipitation line developed between the low molecular weight yglobulins and antiserum to F(ab')2 con cordant with earlier results [14].…”

Section: Experiments and Resultssupporting

confidence: 87%

“…These antibodies are of the yG class as judged from the result of experiments using fractions obtained by gel filtration and ultracentrif ugation of pooled cord serum. In addition, low molecular weight antibodies, approximately 3 S, with similar specificity were demonstrat-cd consistent with earlier findings with human adult sera [13,14]. Similar to yG globulins, the low molecular weight antibodies are resistant to treatment with ME.…”

Section: Discussionsupporting

confidence: 89%

“…The low molecular weight antibodies involved seem to lack the capac ity to react with rheumatoid factor. However, since they fix comple ment, pass placenta and precipitate in agar with antiserum to F(ab').,, these particular yglobulins contain at least part of the Fc-fragment of the heavy chains in addition to light chains [14].…”

Section: Discussionmentioning

confidence: 99%

“…These antibodies belong to the yM and yG classes of immunoglobulins [13]. Low molecular weight antibodies, approximate ly 3 S, with similar specificity have also been described [14]. The antibody activity was determined by immune adherence with skin sections (adherence activity) [12], and by agglutination of sheep erythrocytes coated with the antigen involved [15].…”

mentioning

confidence: 96%

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The Occurrence of Antibodies to Stratum corneum in Man

Krogh¹

1970

Int Arch Allergy Immunol

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Section: Experiments and Resultssupporting

confidence: 92%

“…By double diffusion in agar a precipitation line developed between the low molecular weight yglobulins and antiserum to F(ab')2 con cordant with earlier results [14].…”

Section: Experiments and Resultssupporting

confidence: 87%

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

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The Occurrence of Antibodies to Stratum corneum in Man

Krogh¹

1970

Int Arch Allergy Immunol

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The sensitivity of antibody to 2‐mercaptoethanol treatment in three Australian marsupials

Yadav¹

1973

Eur J Immunol

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Antibody in Australian marsupials 359Saturation of most of the relevant AMS receptors occurred at a serum concentration of about 1.5 % as suggested b y the biphasic curve obtained by AMS titration (Fig. 3). This concentration could be quantitively related t o the number of cells in the culture (unpublished data). The steep slope observed a t low AMS concentrations was probably due to the binding of antibodies to the free macrophage receptors. At a serum concentration of about 1.5 %, receptor saturation seemed complete. The moderate slope at higher concentrations was probably due t o different equilibrium states between AMS receptors and the antibodies in excess. A similar titration of antiserum might b e a useful method for the estimation of the relative amounts of antibody receptors present on the cell surface.The shedding and subsequent reappearance of the relevant antigens on the cell surface suggests that a dynamic state exists in macrophage membranes. The physiological significance of the phenomenon of blocking antibody activity by cells against which they are directed is subject t o speculation. Possibly, the escape of either malignant or normal cells from antibody-mediated cytotoxic effect is due t o a similar blocking that takes place in vivo.The shedding of antigens from cultured cells into their growth media occurs most likely in other cell systems. Separation of these components from the culture media may be a valuable method t o isolate and characterize membranal antigens. We wish to express our gratitude to Prof: P. Minden for his critical review of the manuscript. l%is study was supported by research grants from Concern Foundation, Mr. and Mrs. F. Lautenberg and Mr. and Mrs. L. Tisch.Immunological responses t o injections of sheep erythrocytes, Salmonella adelaide flagella and bacteriophage @X 174 were investigated in three species of Western Australian marsupials (Setonix brachyurus, Macropus eugenii and Trichosurus uulpecula).Two classes of immunoglobulins similar t o the eutherian IgM and IgG were normally formed t o the antigens. In addition, a low molecular weight anti-phage a x 1 7 4 antibody of about 80 000 daltons was present in the three species. In Setonix the 19 S antibody converted t o 7 S type relatively slowly in the primary response, but rapidly in the secondary response. The 2-mercaptoethanol treatment showed that both 19 S and 7 S antibody was sensitive in variable proportions ranging from 0 t o 100 % and a different lability was noted t o each of the antigens in the three species.In general, results indicated that the immune response in the marsupials did not lack the important features which typify an immune response in eutherians.

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Autoimmune Diseases of the Skin and Skin Disorders with Diverse Immuno-Pathological Abnormalities

Korossy¹

1975

Immunological Aspects of Allergy and Allergic Diseases

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Low Molecular Weight Antibodies in Human Sera to Stratum corneum

Cited by 8 publications

References 6 publications

The Occurrence of Antibodies to Stratum corneum in Man

The Occurrence of Antibodies to Stratum corneum in Man

The sensitivity of antibody to 2‐mercaptoethanol treatment in three Australian marsupials

Autoimmune Diseases of the Skin and Skin Disorders with Diverse Immuno-Pathological Abnormalities

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