Low molecular weight allergens of the pollen of parietaria officinalis

Allergenic pollen in europe

“…In Northern Europe pollen from representatives of the Betulaceae (birch, alder, hazel) is a significant contributor to the incidence of pollinosis, whereas Parietaria and olive (Olea) (Arobba & Ruffilli et al 1987). Qn clinical ground, however, these differences are irrelevant.…”

Pollen‐related allergy in the European Mediterranean area

“…These were isolated by affinity chromatography with a mouse monoclonal antibody. Low molecular weight IgE-binding components were separated by gel filtration of P. Judaica and P. officinalis pollen extracts [59,60]. Leaves and stems of P. Judaica have also been found to have allergenic quality [61].…”

Isolation and in vitro Translation of mRNA from Inflorescences of <i>Parietaria judaica</i>

“…For the an alysis of coprecipitation of the translated radioactive proteins with carrier IE, prior to electrophoresis, 0.5 ml of the IE containing 0.05 mg/ml of protein was incubated at room temperature for 30 min with 6 pi of phosphate-buffered saline containing 0.3 mg/ml L-methionine, 1 mg/ml ovalbumin and 10 pi of the la belled translation mixture [9]. The gels were dried and autoradiographed a t -80°C.…”

“…RAST and RAST inhibition assays were carried out as de scribed by Ruffilli et al [9], Experimental details are given in the legend to figure I. Inhibition was expressed as:…”