Low molecular weight albumins from sunflower seed: identification of a methionine-rich albumin

Kortt, Alexander A.; Caldwell, J. B.

doi:10.1016/0031-9422(90)87080-e

Cited by 82 publications

(71 citation statements)

References 29 publications

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“…CPTI contains a low amount of histidine and methionine, characteristic of vegetal proteins (23,24). Isolated Kunitz inhibitors from G. max (21) and A. confusa (22) have two histidine residues each and two and no methionine residues, respectively.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a New Trypsin Inhibitor from Crotalaria paulina Seeds

Pando

Ciero²,

Novello

et al. 1999

IUBMB Life

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SummaryA new trypsin inhibitor (CPTI) has been isolated from Crotalaria paulina seeds. Puri cation of the inhibitor was carried out by gel ltration, ion-exchange chromatography, and subsequent reversed-phase HPLC. The presence of a single polypeptide chain, with a molecular mass of 20 kDa and isoelectric point 4.0, was detected. The trypsin inhibitor had a K i value of 4.5 £ 10 ¡ 8 M and was capable of acting on human, bovine, and porcine trypsin and weakly on bovine chymotrypsin. Amino acid analysis showed that CPTI has a high content of aspartate, glutamate, leucine, serine, and glycine, having 177 amino acid residues in its composition. These data suggest that the protein belongs to the Kunitz-type trypsin inhibitors. IUBMB Life, 48: 519-523, 1999

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a New Trypsin Inhibitor from Crotalaria paulina Seeds

Pando

Ciero²,

Novello

et al. 1999

IUBMB Life

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“…Variant types of 2s albumin also occur. Those of pea appear to lack interchain disulfide bonds (Higgins et al, 1986), whereas the 2s albumins of sunflower remain uncleaved (Figures l c and le; Kortt and Caldwell, 1990;Anisimova et al, 1994). In addition, in sunflower and castor bean, some mRNAs encode two mature albumin proteins, each consisting of one or two subunits (Figures l d and le; Allen et al, 1987;lrwin et al, 1990; I?…”

Section: S Albumin Storage Proteinsmentioning

confidence: 99%

Seed storage proteins: structures and biosynthesis.

1995

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“…11 -13 Variant types of 2 S albumins, such as the sunflower 8 2 S albumin (SFA8) described here, are not cleaved in planta into large and small subunits but remain as a single monomeric subunit. 14,15 Although some functions have been assigned to some 2 S albumins, their specific biological roles are mostly unknown. 8 The allergenicity of Ber e 1 is well-established.…”

Section: Introductionmentioning

confidence: 99%

The Disulphide Mapping, Folding and Characterisation of Recombinant Ber e 1, an Allergenic Protein, and SFA8, Two Sulphur-rich 2S Plant Albumins

Alcocer

Murtagh

Bailey

et al. 2002

Journal of Molecular Biology

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We have cloned and expressed genes encoding the allergenic brazil nut 2 S albumin (Ber e 1) and the sunflower albumin 8 (SFA8) in the methylotrophic yeast Pichia pastoris. We show that both proteins were secreted at high levels and that the purified proteins were properly folded. We also showed that Ber e 1 is glycosylated during secretion and that the glycan does not interfere with the folding or immunoreactivity. The disulphide map of the Ber e 1 protein was experimentally established and is in agreement with the conserved disulphide structure of other members of the 2 S albumin family. A model three-dimensional structure of the allergen was generated. During the expression studies and through mutation we have also shown that alteration of the sequences around the Kex2 endoproteolytic processing site in the expressed fusion protein can compromise the secretion by targeting part of the protein for possible degradation. The secreted production of these properly folded sulphurrich plant albumins presents an opportunity to delineate the attributes that make an allergen and to facilitate the diagnosis and therapy of type I allergy.

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Low molecular weight albumins from sunflower seed: identification of a methionine-rich albumin

Cited by 82 publications

References 29 publications

Isolation and Characterization of a New Trypsin Inhibitor from Crotalaria paulina Seeds

Isolation and Characterization of a New Trypsin Inhibitor from Crotalaria paulina Seeds

Seed storage proteins: structures and biosynthesis.

The Disulphide Mapping, Folding and Characterisation of Recombinant Ber e 1, an Allergenic Protein, and SFA8, Two Sulphur-rich 2S Plant Albumins

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