Low Melatonin Production During Adulthood - Phase 2: Association with Levels of Hydroxyl Radical Scavenging and DNA Damage

Abstract

Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, search'ng existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send commrrents regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Washington Headquarters Services, Directorate The primary purpose of the proposed study is to develop cross-sectional evidence concerning whether or not lower melatonin production levels are associated with increased oxidative DNA guanine damage. If the results of this study are supportive, then confirmatory studies would be warranted, followed by prospective chemoprevention studies of melatonin supplementation. Adjuvant cancer treatment studies have not identified any serious melatonin toxicities.High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS) will be used to simultaneously quantitate urinary levels of . This assay appears superior to both the comet and the HPLC-ECD assays. Assays to estimate melatonin production have already been performed in Phase 1. The existing complete overnight urine samples have been properly processed and stored for the proposed study. Fiftyfive (55) mother-daughter-father triples of urine samples along with detailed epidemiologic questionnaires are available. SUBJECT TERMS NUMBER OF PAGESDNA damage, urinary metabolites, urinary melatonin metabolites 5