2002
DOI: 10.1182/blood-2002-01-0271
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Low levels of ABCG2 expression in adult AML blast samples

Abstract: Previous reports have suggested that the adenosine triphosphate-binding cassette protein ABCG2 (breast cancer resistance protein [BCRP], mitoxantrone resistance [MXR]) is associated with drug resistance in acute myeloid leukemia (AML). The aims of this study were to determine the level of ABCG2 mRNA expression necessary to produce drug resistance and to define the ABCG2 levels in normal bone marrow (BM), peripheral blood (PB), cord blood (CB), and adult AML blast cell populations. First, using transduced clona… Show more

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Cited by 101 publications
(92 citation statements)
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References 16 publications
(32 reference statements)
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“…Though verapamil is generally used to identify the SP phenotype in the Hoechst 33342 dye exclusion assay, it is insufficient in specificity to demonstrate the contribution of ABCG2 to the SP phenotype of limbal epithelial cells. As ABCG2-specific inhibitors, fumitremorgin C [33], 5D3 monoclonal antibody [13,22], and TPS-A [23] are reported. Our SP analysis of limbal epithelial cells using 5D3 and TPS-A showed that both 5D3 and TPS-A effectively inhibit Hoechst 33342 dye efflux from these cells (Fig.…”
Section: Discussionmentioning
confidence: 99%
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“…Though verapamil is generally used to identify the SP phenotype in the Hoechst 33342 dye exclusion assay, it is insufficient in specificity to demonstrate the contribution of ABCG2 to the SP phenotype of limbal epithelial cells. As ABCG2-specific inhibitors, fumitremorgin C [33], 5D3 monoclonal antibody [13,22], and TPS-A [23] are reported. Our SP analysis of limbal epithelial cells using 5D3 and TPS-A showed that both 5D3 and TPS-A effectively inhibit Hoechst 33342 dye efflux from these cells (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…When SP cells were incubated with an anti-ABCG2 monoclonal antibody, 5D3, recognizing an external plasma membrane epitope on living cells [13,22], prior to Hoechst 33342 dye exclusion assay, a marked decrease in SP cell dye efflux was observed ( Fig. 2A).…”
Section: Effect Of 5d3 and Tps-a On Hoechst 33342 Dye Effluxmentioning
confidence: 99%
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“…Chimeric fusion proteins containing two ABCG2 monomers either with or without a flexible linker peptide were shown to be functional (13) supporting the idea of homodimer formation. In addition, coimmunoprecipitation experiments using two different tags on the ABCG2 monomers also suggested homodimer formation (31). A disulfide bridge formed between cysteines 603 of the monomers in the predicted extracellular loop connecting TM5 and TM6 was described as non-essential for trafficking and transport (14).…”
mentioning
confidence: 99%
“…There are 49 human genes in the ATP-binding cassette (ABC) transporter superfamily encoding transmembrane proteins, which mediate drug efflux via ATP-dependent pumps (17)(18)(19)(20)(21). Among the ABC transporters, expression of multidrug resistance protein 1 (MDR1 or P-glycoprotein) and breast cancer resistance protein 1 (BCRP1) have been associated with chemotherapy resistance in AML in some studies (22)(23)(24)(25)(26)(27)(28) although not in all (29)(30)(31)(32). One possible reason for the conflicting prognostic relevance suggested for ABC transporter expression in AML is the variety of methods used to assess that expression in previous studies (33).…”
Section: Introductionmentioning
confidence: 99%