Low levels and partial exposure to palmitic acid improves mitochondrial function and the oxidative status of cultured cardiomyoblasts

Mthembu, Sinenhlanhla X.H.; Mazibuko-Mbeje, Sithandiwe E.; Silvestri, Sonia; Orlando, Patrick; Marcheggiani, Fabio; Cirilli, Ilenia; Nkambule, Bongani B.; Muller, Christo J.F.; Tiano, Luca; Dludla, Phiwayinkosi V.

doi:10.1016/j.toxrep.2024.01.014

Cited by 5 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The predominate component in essential oil, palmitic acid (30.74%), a saturated fatty acid, has been reported to exhibit antibacterial activity by impairing the bacterial cell membrane, causing the leakage of cellular contents and ultimately resulting in bacterial death [ 46 ]. Additionally, low concentrations of palmitic acid exert a protective antioxidant effect in cardiomyoblasts, suggesting its potential to protect the heart from oxidative stress [ 47 ]. The second most abundant compound is linolenic acid (16.13%), an unsaturated fatty acid commonly found in nature, which can attenuate Alzheimer’s disease pathology, such as tau phosphorylation, blood–brain barrier disruption, synaptic dysfunction, and cognitive impairment.…”

Section: Resultsmentioning

confidence: 99%

Spermacoce alata Aubl. Essential Oil: Chemical Composition, In Vitro Antioxidant Activity, and Inhibitory Effects of Acetylcholinesterase, α-Glucosidase and β-Lactamase

Zhu,

et al. 2024

Molecules

View full text Add to dashboard Cite

Spermacoce alata Aubl. is widely available in the market as traditional Chinese medicine and animal feed, due to its properties of clearing heat and treating malaria and its high-protein and crude fiber content. In this study, the essential oil of S. alata was obtained through hydrodistillation. GC–MS and GC–FID methods were used to identify the chemical components and their relative abundance. Furthermore, the antioxidant capacity was measured using DPPH, ABTS, and FRAP assays, and the inhibitory effects of acetylcholinesterase, α-glucosidase, and β-lactamase were also evaluated. A total of 67 compounds were identified, with the major constituents being palmitic acid (30.74%), linoleic acid (16.13%), and phenylheptatriyne (8.07%). The essential oil exhibited moderate antioxidant activity against DPPH (IC50 > 10 mg/mL), while the IC50 value for the ABTS assay was 3.84 ± 2.12 mg/mL and the FRAP assay value was 87.22 ± 12.22 µM/g. Additionally, the essential oil showed moderate anti-acetylcholinesterase activity (IC50 = 286.0 ± 79.04 μg/mL), significant anti-α-glucosidase activity (IC50 = 174.7 ± 13.12 μg/mL), and potent anti-β-lactamase activity (IC50 = 37.56 ± 3.48 μg/mL). The results suggest that S. alata has the potential for application in pharmacology, warranting further exploration and investigation.

show abstract

Section: Resultsmentioning

confidence: 99%

Spermacoce alata Aubl. Essential Oil: Chemical Composition, In Vitro Antioxidant Activity, and Inhibitory Effects of Acetylcholinesterase, α-Glucosidase and β-Lactamase

Zhu,

et al. 2024

Molecules

View full text Add to dashboard Cite

show abstract

“…PA may also support the proper functioning of the immune system [12]. However, it must be emphasized that the positive effect of this compound can be observed only for small doses of PA [13][14][15]. Contrarily, a high consumption of PA in the daily diet may have a negative impact on the human body, contributing to an increase in the concentration of total cholesterol in the blood serum and an increase in its LDL (low-density lipoprotein) fraction [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Raman Imaging—A Valuable Tool for Tracking Fatty Acid Metabolism—Normal and Cancer Human Colon Single-Cell Study

Beton-Mysur,

Kopec,

Brozek-Pluska

2024

IJMS

View full text Add to dashboard Cite

Altered metabolism of lipids is a key factor in many diseases including cancer. Therefore, investigations into the impact of unsaturated and saturated fatty acids (FAs) on human body homeostasis are crucial for understanding the development of lifestyle diseases. In this paper, we focus on the impact of palmitic (PA), linoleic (LA), and eicosapentaenoic (EPA) acids on human colon normal (CCD-18 Co) and cancer (Caco-2) single cells using Raman imaging and spectroscopy. The label-free nature of Raman imaging allowed us to evaluate FAs dynamics without modifying endogenous cellular metabolism. Thanks to the ability of Raman imaging to visualize single-cell substructures, we have analyzed the changes in chemical composition of endoplasmic reticulum (ER), mitochondria, lipid droplets (LDs), and nucleus upon FA supplementation. Analysis of Raman band intensity ratios typical for lipids, proteins, and nucleic acids (I1656/I1444, I1444/I1256, I1444/I750, I1304/I1256) proved that, using Raman mapping, we can observe the metabolic pathways of FAs in ER, which is responsible for the uptake of exogenous FAs, de novo synthesis, elongation, and desaturation of FAs, in mitochondria responsible for energy production via FA oxidation, in LDs specialized in cellular fat storage, and in the nucleus, where FAs are transported via fatty-acid-binding proteins, biomarkers of human colon cancerogenesis. Analysis for membranes showed that the uptake of FAs effectively changed the chemical composition of this organelle, and the strongest effect was noticed for LA. The spectroscopy studies have been completed using XTT tests, which showed that the addition of LA or EPA for Caco-2 cells decreases their viability with a stronger effect observed for LA and the opposite effect observed for PA. For normal cells, CCD-18 Co supplementation using LA or EPA stimulated cells for growing, while PA had the opposite impact.

show abstract

Carissa spinarum seed oil: a newer perspective on dietary lipids and nutraceuticals – phytochemical characterization, Lipidomics, and pre-toxicological assessment

Prabhakaran,

Selvi,

Vijayaraj

et al. 2024

Food Measure

View full text Add to dashboard Cite

Low levels and partial exposure to palmitic acid improves mitochondrial function and the oxidative status of cultured cardiomyoblasts

Cited by 5 publications

References 41 publications

Spermacoce alata Aubl. Essential Oil: Chemical Composition, In Vitro Antioxidant Activity, and Inhibitory Effects of Acetylcholinesterase, α-Glucosidase and β-Lactamase

Spermacoce alata Aubl. Essential Oil: Chemical Composition, In Vitro Antioxidant Activity, and Inhibitory Effects of Acetylcholinesterase, α-Glucosidase and β-Lactamase

Raman Imaging—A Valuable Tool for Tracking Fatty Acid Metabolism—Normal and Cancer Human Colon Single-Cell Study

Carissa spinarum seed oil: a newer perspective on dietary lipids and nutraceuticals – phytochemical characterization, Lipidomics, and pre-toxicological assessment

Contact Info

Product

Resources

About