Low-level light-emitting diode therapy increases mRNA expressions of IL-10 and type I and III collagens on Achilles tendinitis in rats

Xavier, Murilo; Souza, Renato Aparecido de; Pires, Viviane Araújo; Santos, Ana Paula; Aimbire, Flávio; Silva, José Antônio da; Albertini, Regiane; Villaverde, Antônio Balbin

doi:10.1007/s10103-013-1280-y

Cited by 24 publications

(21 citation statements)

References 31 publications

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“…Xavier et al [30] employed Raman spectroscopy to assess the quantitative changes in the composition of collagen types I and III in tendons, as a result of the effects of lowintensity phototherapy in an experimental model of tendinitis in rats, treated with laser at 780 nm and LED at 880 nm. Moura Júnior et al [29] used the FT-Raman spectra to estimate the amount of collagen types I and III in the tendons of rats submitted to an experimental model of tendonitis and treated with LED therapy at 625 nm, and a daily dose of 4 J/ cm 2 at the same point, compared to therapeutic US with 0.5 W/cm 2 and control.…”

Section: Introductionmentioning

confidence: 99%

“…The use of Raman spectroscopy as a diagnostic tool for pathological changes has been proposed for the study of various biological tissues, including tendons, which have been of great potential to study acute tendinitis at different experimental periods [23,[29][30][31][32]. The technique has been applied to assess the structural changes in tendons and to determine the composition of collagen and quantify collagen types I and III in tendon tissues submitted to light therapy compared to controls [30].…”

Section: Introductionmentioning

confidence: 99%

“…Among these techniques, Raman spectroscopy has been proposed for the biochemical analysis of biological tissues, mainly because of the low interference with the water and the ability to unveil the chemical structure by measuring the vibration energy of the molecules [24][25][26][27][28]. The use of Raman spectroscopy as a diagnostic tool for pathological changes has been proposed for the study of various biological tissues, including tendons, which have been of great potential to study acute tendinitis at different experimental periods [23,[29][30][31][32]. The technique has been applied to assess the structural changes in tendons and to determine the composition of collagen and quantify collagen types I and III in tendon tissues submitted to light therapy compared to controls [30].…”

Section: Introductionmentioning

confidence: 99%

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Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy

Júnior¹,

Filho²,

Pessoa³

et al. 2015

Lasers Med Sci

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Given the complexity of the tendon repair process, where the neoformed scar tissue tends to prevent or hamper the tendon from performing its normal functions, ultrasound (US) and light-emitting diode (LED) devices have been applied to modulate the tendon repair process. This study aimed to compare the anti-inflammatory and healing effects of therapeutic US and LED in an experimental model of tenotomy using histomorphometry and Raman spectroscopy. Forty Wistar rats (235 ± 8.3 g) were separated into four groups: tenotomy (TC; control), tenotomy associated with ultrasound (US; 1 MHz, 0.5 W/cm(2)), tenotomy associated with LED at 625 nm (LR; 4 J/cm(2)), and tenotomy associated with LED at 945 nm (LIR; 4 J/cm(2)), in two experimental periods of 7 and 14 days. Histomorphometry showed a decrease of inflammatory cells (macrophages and polymorphonuclear leukocytes) for the group treated with US compared to the TC group (ANOVA, p < 0.0001) and the LIR group compared to the TC group (p < 0.05) at the seventh day, indicating an anti-inflammatory effect. In relation to the LR and TC groups, there was no significant difference. Raman spectroscopy revealed that the LR group presented an increased synthesis of collagen I compared to the other treatments and the TC groups on the 14th day (p < 0.01). US therapy showed an adjuvant effect with anti-inflammatory action, and the LED therapy at 625 nm showed an increase in collagen synthesis, contributing to the process of tendon healing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy

Júnior¹,

Filho²,

Pessoa³

et al. 2015

Lasers Med Sci

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“…Sabe-se que quando o tendão é posto em sobrecarga ele perde a sua capacidade reparadora basal podendo levar à micro traumas cumulativos que enfraquecem as ligações do colágeno, a matriz não-colágena e os elementos vasculares do tendão 3 . Diversos recursos terapêuticos como Laser 4,5 , Led e ultrassom terapêutico isolado 6,7 , combinados à fármacos e fitoterápicos 6,8 por meio da sonoforese tem sido utilizados na inflamação e reparo de tendão.…”

Section: Introductionunclassified

Eficácia Da Fonoforese Com Ximenia Americana L. Na Inflamação De Tendão De Ratos

Leal

Uchôa

Figuerêdo-Silva

et al. 2016

Rev Bras Med Esporte

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Introdução: Diversos recursos terapêuticos, como laser e ultrassom isolado, combinados com fármacos e fonoforese têm sido utilizados em casos de inflamação e reparo de tendão, sendo o ultrassom pulsado bastante eficaz em tendinites crônicas. Objetivo: Analisar a eficácia do uso da fonoforese com o extrato etanólico das cascas do caule da Ximenia americana L. na resolução do processo inflamatório crônico em Rattus norvegicus. Métodos: A amostra consistiu em 120 animais para análise da resposta inflamatória, utilizando-se como variáveis edema, força biomecânica, número de fibroblastos e análise histológica. Resultados: Nos 7° e no 14° dia, verificou-se diferença significativa entre o grupo ultrassom com gel do caule de Ximenia americana L. e o grupo ultrassom com placebo (p < 0,05) quanto à redução de edema, aumento da força máxima de ruptura, redução extremamente significativa da deformação máxima (p < 0,001), além de aumento dos fibroblastos. Na análise histológica, houve melhora do processo inflamatório inicial e aceleração do reparo tendíneo, com redução de células inflamatórias e com deposição de colágeno organizado com matriz extracelular densa. Conclusão: O ultrassom pulsado combinado com o gel do caule de Ximenia americana L. é uma forma terapêutica eficaz para a resolução do processo inflamatório crônico.Descritores: fonoforese; olacaceae; inflamação; tendão do calcâneo. ABSTRACT Introduction: Several therapeutic resources such as laser and isolated ultrasound combined with drugs and phonophoresis have been used in cases of inflammation and tendon repair, and the pulsed ultrasound is quite effective in chronic tendinitis. Objective: To analyze the efficacy of phonophoresis with the ethanol extract of the stem bark of Ximenia americana L. to manage the chronic inflammatory process in Rattus norvegicus

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“…LLLT started 12 h after the second injection of collagenase (4th day), according to the previous study, which started treatment on collagenase-induced tendinitis after 12 h, 31 and was repeated daily, during 14 days. The equipment used in the study was a Twin Flex Evolution (MMOptics, São Carlos, SP, Brazil), possessing laser wavelengths of 660 and 780 nm.…”

Section: Laser Therapymentioning

confidence: 99%

Effect of Low-Level Laser Therapy in an Experimental Model of Osteoarthritis in Rats Evaluated Through Raman Spectroscopy

Mangueira

Xavier²,

Souza³

et al. 2015

Photomedicine and Laser Surgery

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Objective: This work aimed to investigate the biochemical changes associated with low-level laser therapy (LLLT) using 660 and 780 nm, on a well-established experimental model of osteoarthritis (OA) in the knees of rats with induced collagenase, using histomorphometry and Raman spectroscopy. Materials and methods: Thirty-six Wistar rats were divided into four groups: control (GCON, n = 9), collagenase without treatment (GCOL, n = 9), collagenase with LLLT 660 nm treatment (G660, n = 8), and collagenase with LLLT 780 nm treatment (G780, n = 10). LLLT protocol was: 30 mW power output, 10 sec irradiation time, 0.04 cm 2 spot size, 0.3 J energy, 0.75 W/cm 2 irradiance, and 7.5 J/cm 2 fluence per session per day, during 14 days. Then, knees were withdrawn and submitted to histomorphometry and Raman spectroscopy analysis. Principal components analysis (PCA) and Mahalanobis distance were employed to characterize the spectral findings. Results: Histomorphometry revealed a significant increase in the amount of collagen III for the group irradiated with 660 nm. The Raman bands at 1247, 1273, and 1453 cm -1 (from principal component score PC2), attributed to collagen type II, and 1460 cm -1 (from PC3), attributed to collagen type III, suggested that the LLLT causes acceleration in cellular activity, especially on the cells that repair cartilage, accelerating the breakdown of cartilage destroyed by collagenase and stimulating the fibroblast to synthesize repairing collagen III. Conclusions: LLLT accelerated the initial breakdown of cartilage destroyed by collagenase and stimulated the fibroblast to synthesize the repairing collagen III, suggesting a beneficial effect of LLLT on OA.

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Low-level light-emitting diode therapy increases mRNA expressions of IL-10 and type I and III collagens on Achilles tendinitis in rats

Cited by 24 publications

References 31 publications

Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy

Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy

Eficácia Da Fonoforese Com Ximenia Americana L. Na Inflamação De Tendão De Ratos

Effect of Low-Level Laser Therapy in an Experimental Model of Osteoarthritis in Rats Evaluated Through Raman Spectroscopy

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