Low level LED photodynamic therapy using curcumin loaded tetraether liposomes

Duse, Lili; Pinnapireddy, Shashank Reddy; Strehlow, Boris; Jedelská, Jarmila

doi:10.1016/j.ejpb.2017.10.005

Cited by 66 publications

(49 citation statements)

References 34 publications

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“…Absorbance was measured at 570 nm in a plate reader (FLUOstar Optima, BMG Labtech, Ortenberg, Germany). Absorbance of untreated cells (blank) was considered as 100% viability …”

Section: Methodsmentioning

confidence: 99%

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Multilayer Bacteriostatic Coating for Surface Modified Titanium Implants

Vögeling

Duse

Seitz

et al. 2018

Physica Status Solidi (a)

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One of the serious problems occurring after the insertion of implants is the formation of biofilms. This leads to implant loss or sepsis. The multilayer coating developed in this study addresses the problems pertaining to bacterial growth and biofilm formation by two different approaches viz. preventing bacterial adhesion by nanostructured surface modification and by modification of the titanium surface by creating an ultra-thin poly(lactic-coglycolic acid) (PLGA)/norfloxacin (NFX) nanoparticle coating. In this study, NFX loaded PLGA nanoparticles are used to coat titanium surfaces using the layer by layer method alternating with chitosan. The advantages of biodegradable polymers like PLGA and chitosan to create an anti-adhesive and antibacterial coating are utilised. The polymers in this study show a high biocompatibility and are completely degraded within 10 weeks which makes them an excellent choice for use in dental implants and stents.

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“…Absorbance was measured at 570 nm in a plate reader (FLUOstar Optima, BMG Labtech, Ortenberg, Germany). Absorbance of untreated cells (blank) was considered as 100% viability …”

Section: Methodsmentioning

confidence: 99%

“…As references Triton X–100 and PBS buffer (pH 7.4) were used. The absorption of Triton X–100 was set as 100% haemolysis …”

Section: Methodsmentioning

confidence: 99%

Multilayer Bacteriostatic Coating for Surface Modified Titanium Implants

Vögeling

Duse

Seitz

et al. 2018

Physica Status Solidi (a)

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“…Research over the last decade has proven that curcumin possesses anti‐oxidative and anti‐inflammatory activities and exhibits a potent activity against numerous chronic diseases . Curcumin has been also intensively investigated as a therapeutic or preventive agent against cancer . Furthermore, using curcumin in the field of PDT as a natural photosensitizer expanded its range of applications .…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Curcumin Loaded Chitosan Nanoparticles for Photodynamic Therapy

Duse

Baghdan

Pinnapireddy

et al. 2017

Physica Status Solidi (a)

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Photodynamic therapy (PDT) is a minimally invasive tumor targeting strategy, in which tumor tissues can be selectively destroyed. Upon excitation with light of a specific wavelength, a photosensitizer can produce reactive oxygen species (ROS) in the presence of molecular oxygen, thereby exhibiting a cytotoxic effect toward the surrounding tissues, giving a total control on the onset of therapy. In this research work, curcumin as a natural photosensitizer has been investigated against cancer cells. To enhance the solubility of curcumin, a nanoformulation is developed using chitosan as biodegradable and biocompatible polymer that has the ability to encapsulate curcumin and improve its therapeutic effect. Physicochemical characterizations revealed that the prepared formulations were in the nanoscale range and had a positive ζ-potential. Upon irradiation using a novel LED device (λ ex ¼ 457 nm), curcumin nanoparticles were able to generate ROS and destroy tumor cells. Moreover, unloaded nanoparticles exhibited nonsignificant phototoxicity. These outcomes confirm the importance of curcumin nanoparticles in enhancing the efficacy of curcumin and offer new insights in the field of PDT against cancer.

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“…This may be explained, at least in part, by a lack of competition and limited market size that has discouraged an exciting and robust industry. Those with more specific irradiation requirements, and the appropriate motivation and funding, have reported custom-assembled LED sources for various applications, 27,[36][37][38][39][40] albeit with limited output powers that require tedious experimental protocols. A light source or system that is cost-effective and generalizable to multiple PSs has not yet been reported.…”

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confidence: 99%

High-power light-emitting diode array design and assembly for practical photodynamic therapy research

Kercher¹,

Zhang²,

Waguespack³

et al. 2020

J. Biomed. Opt.

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Q. Spring, "High-power light-emitting diode array design and assembly for practical photodynamic therapy research," J. AbstractSignificance: Commercial lasers, lamps, and light-emitting diode (LED) light sources have stimulated the clinical translation of photodynamic therapy (PDT). Yet, the continued exploration of new photosensitizers (PSs) for PDT often requires separate activation wavelengths for each agent being investigated. Customized light sources for such research frequently come at significant financial or technical cost, especially when compounded over many agents and wavelengths.Aim: LEDs offer potential as a cost-effective tool for new PS and multi-PS photodynamic research. A low-cost-per-wavelength tool leveraging high-power LEDs to facilitate efficient and versatile research is needed to further accelerate research in the field.Approach: We developed and validated a high-power LED array system for benchtop PDT with a modular design for efficient switching between wavelengths that overcome many challenges in light source design. We describe the assembly of a low-cost LED module plus the supporting infrastructure, software, and protocols to streamline typical in vitro PDT experimentation.Results: The LED array system is stable at intensities in excess of 100 mW∕cm 2 with 2.3% variation across the illumination field, competitive with other custom and commercial devices. To demonstrate efficacy and versatility, a primary ovarian cancer cell line was treated with two widely used PSs, aminolevulinic acid and verteporfin, using the LED modules at a clinically relevant 50 J∕cm 2 light dose that induced over 90% cell death for each treatment. Conclusions:Our work provides the community with a tool for new PS and multi-PS benchtop photodynamic research that, unlike most commercial light sources, affords the user a low barrier to entry and low-cost-per-wavelength with the goal of illuminating new insights at the forefront of PDT.

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Low level LED photodynamic therapy using curcumin loaded tetraether liposomes

Cited by 66 publications

References 34 publications

Multilayer Bacteriostatic Coating for Surface Modified Titanium Implants

Multilayer Bacteriostatic Coating for Surface Modified Titanium Implants

Preparation and Characterization of Curcumin Loaded Chitosan Nanoparticles for Photodynamic Therapy

High-power light-emitting diode array design and assembly for practical photodynamic therapy research

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