Low-level laser therapy induces human umbilical vascular endothelial cell proliferation, migration and tube formation through activating the PI3K/Akt signaling pathway

Li, Yue; Xu, Qingmei; Shi, Meng-Lin; Gan, Puying; Huang, Qin; Wang, Anan; Tan, Guangming; Fang, Yangbin; Liao, Hongfei

doi:10.1016/j.mvr.2019.103959

Cited by 51 publications

(34 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was confirmed that the radiation at a wavelength of 633 nm increased the angiogenesis of HUVEC by irradiation [27]. The LLLT was shown to accelerate the proliferation, movement, and angiogenesis of HUVECs by activating the PI3K/Akt signal transmission pathway [28]. Our study also confirmed that the tube formation of HUVEC was increased by PBMT.…”

Section: Discussionsupporting

confidence: 78%

Development of a Patch-Type Sensor for Skin Using Laser Irradiation Based on Tissue Impedance for Diagnosis and Treatment of Pressure Ulcer

et al. 2021

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 78%

Development of a Patch-Type Sensor for Skin Using Laser Irradiation Based on Tissue Impedance for Diagnosis and Treatment of Pressure Ulcer

et al. 2021

View full text Add to dashboard Cite

“…In our study, we demonstrated that CW‐703 suppressed HUVEC cell migration and tube formation even in its low dose, and it has inhibitory effect on cell proliferation at the dose of 100 μM. Studies have shown that PI3K/AKT/VEGF pathway, via which CW‐703 might suppress angiogenesis in our study, was involved in endothelial cell proliferation, migration and also tube formation 31,37‐39 . Therefore, CW‐703 might have an inhibitory impact on all the three steps of angiogenesis formation with different potencies.…”

Section: Discussionmentioning

confidence: 56%

“…Studies have shown that PI3K/AKT/VEGF pathway, via which CW-703 might suppress angiogenesis in our study, was involved in endothelial cell proliferation, migration and also tube formation. 31,[37][38][39] Therefore, CW-703 might have an inhibitory impact on all the three steps of angiogenesis formation with different potencies.…”

Section: Discussionmentioning

confidence: 99%

Novel peptide derived from IGF‐2 displays anti‐angiogenic activity in vitro and inhibits retinal angiogenesis in a model of oxygen‐induced retinopathy

Zheng

Sun

2020

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

Background Retinopathy of prematurity (ROP), a major cause of significant visual morbidity and blindness in preterm infants, is closely related to pathological angiogenesis. The aim of the study is to evaluate the effect of a new 12‐aa peptide (named peptide CW‐703) from human insulin‐like growth factor‐2, against angiogenesis in ROP. Methods In order to evaluate the inhibitory effect of CW‐703 on the proliferation, migration, tube formation and apoptosis of human umbilical vein endothelial cells (ScienCell) in vitro, we used MTS assays, a modified Boyden chamber, Matrigel system and TUNEL assays. Effects in vivo were assayed using chorioallantoic membrane assays and oxygen‐induced retinopathy (OIR) models in mice. We also performed eletrophysiological and histologic examinations to evaluate the possible toxicity of the peptide. Real‐time PCR, ELISA and western blotting were used to elucidate the mechanism of CW‐703. Results CW‐703 inhibited angiogenesis in vitro by suppressing endothelial cell proliferation, migration and tube formation. CW‐703 also prevented angiogenesis in chicken chorioallantoic membrane assays and OIR assays in mice. No evident functional or morphologic abnormalities in neuroretina after CW‐703 injection were revealed in electrophysiological tests and histological examinations. Moreover, we elucidated that CW‐703 competed for binding to IGF‐1R and inhibited angiogenesis by inhibiting IGF‐1R/PI3K/AKT activation and downregulating vascular endothelial growth factor expression. Conclusion The novel peptide CW‐703 may act as an effective inhibitor of ocular pathologic angiogenesis, especially in treating ROP.

show abstract

“…In addition, at the cellular and tissue levels, there are research reports related to promotion of wound healing, such as cell proliferation of fibroblasts [ 15 , 20 ], osteoblasts [ 22 , 26 ] and vascular endothelial cells [ 18 , 27 ] by LLLT. However, clinical application in medical and dental treatments has not been carried out much.…”

Section: Discussionmentioning

confidence: 99%

Chronological Gene Expression of Human Gingival Fibroblasts with Low Reactive Level Laser (LLL) Irradiation

Wada

Suzuki

Ishiguro

et al. 2021

JCM

View full text Add to dashboard Cite

Though previously studies have reported that Low reactive Level Laser Therapy (LLLT) promotes wound healing, molecular level evidence was uncleared. The purpose of this study is to examine the temporal molecular processes of human immortalized gingival fibroblasts (HGF) by LLLT by the comprehensive analysis of gene expression. HGF was seeded, cultured for 24 h, and then irradiated with a Nd: YAG laser at 0.5 W for 30 s. After that, gene differential expression analysis and functional analysis were performed with DNA microarray at 1, 3, 6 and 12 h after the irradiation. The number of genes with up- and downregulated differentially expression genes (DEGs) compared to the nonirradiated group was large at 6 and 12 h after the irradiation. From the functional analysis results of DEGs, Biological Process (BP) based Gene Ontology (GO), BP ‘the defense response’ is considered to be an important process with DAVID. Additionally, the results of PPI analysis of DEGs involved in the defense response with STRING, we found that the upregulated DEGs such as CXCL8 and NFKB1, and the downregulated DEGs such as NFKBIA and STAT1 were correlated with multiple genes. We estimate that these genes are key genes on the defense response after LLLT.

show abstract

Low-level laser therapy induces human umbilical vascular endothelial cell proliferation, migration and tube formation through activating the PI3K/Akt signaling pathway

Cited by 51 publications

References 27 publications

Development of a Patch-Type Sensor for Skin Using Laser Irradiation Based on Tissue Impedance for Diagnosis and Treatment of Pressure Ulcer

Development of a Patch-Type Sensor for Skin Using Laser Irradiation Based on Tissue Impedance for Diagnosis and Treatment of Pressure Ulcer

Novel peptide derived from IGF‐2 displays anti‐angiogenic activity in vitro and inhibits retinal angiogenesis in a model of oxygen‐induced retinopathy

Chronological Gene Expression of Human Gingival Fibroblasts with Low Reactive Level Laser (LLL) Irradiation

Contact Info

Product

Resources

About