Low-level laser therapy as a modifier of erythrocytes morphokinetic parameters in hyperadrenalinemia

Deryugina, А. V.; Ivashchenko, M.N.; Ignatiev, P. S.; Balalaeva, Irina V.; Самоделкин, А. Г.

doi:10.1007/s10103-019-02755-y

Cited by 15 publications

(22 citation statements)

References 43 publications

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“…Both the significant decrease in the MCH and the increase in the red cell distribution range in the blood (RDW) are directly related to the decrease in MCV in the experimental groups. On the other hand, the mean corpuscular concentration in each erythrocyte (MCHC) remained similar in all groups, as well as the total dosages of Hb and Ht, which may suggest a beneficial effect of LLLT on the levels of Hb and Ht, 15,16 after the previous radiotherapy that has a deleterious effect on blood cells. 12,17,18 This result may be related to the photobiostimulator effect of the laser at a wavelength of 660 nm, which can cause a reaction with Hb since it has chromophoric receptors that are activated in the presence of light.…”

Section: Discussionmentioning

confidence: 78%

Effect of Radiotherapy and Low-Level Laser Therapy on Circulating Blood Cells of Rats

et al. 2021

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Introduction: This study aimed to investigate the effect of low-level laser therapy (LLLT) on the blood cell count when applied to parotid glands of rats irradiated by volumetric modular arc therapy (VMAT). Methods: Thirty-two adult male Wistar rats were used in this study. Samples were randomly assigned to three groups: control group (CG, n = 8), immediate laser group (24 hours) (ILG, n=12), and late laser group (120 hours) (LLG, n=12). The two laser groups were previously subjected to VMAT radiotherapy in a single dose of 12 Gy. LLLT with an AsGaAl laser (660 nm, 100 mW) was applied at three points in the region of the parotid glands, right side, with the energy of 2 J per point (20s, 70 J/cm2 ) and a spot size of 0.0028 cm2 for 10 consecutive days. In the euthanasia, blood samples were obtained by cardiac puncture. The samples from each group were processed by an automatic method and analyzed for erythrogram, leukogram and platelet count values. The data were analyzed by ANOVA and each LLLT time point was analyzed in relation to the control group, with a significance level less than 0.05. Results: Groups using LLLT had higher red blood cell counts, being higher in the LLG (p = 0.000). The hematimetric indices MCV (P=0.002) and MCH (P=0.009) were lower than the control group, especially when compared to the group using LLLT 120h after radiotherapy (LLG). White blood cell counts were lower in the groups with radiotherapy and immediate use of LLLT (ILG) (P=0.011), mainly at the expense of lymphocytes (P=0.002). Conclusion: The results suggest a potential systemic effect of LLLT, especially on circulating red blood cell counts, regardless of their time of immediate or late use of radiotherapy.

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Section: Discussionmentioning

confidence: 78%

Effect of Radiotherapy and Low-Level Laser Therapy on Circulating Blood Cells of Rats

et al. 2021

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“…Discussing the mechanisms of changes in the phase portrait and EPME in various pathologies before and after the treatment, it may be stated that these measurements do not depend on the etiology of the disease and are of the typical character. Based on the previously found dependence of EPME on the stress reaction development and triggering of the adaptive processes [9,10] it may be considered that emergence of echiocytic cell transformation and EPME reduction is caused by the development of stress reaction the intensity of which is manifested by a significant EPME decrease and appearance of spheroechinocytes. But in Thus, the results obtained allow us to state that during the development of a pathological process, stress reaction is progressing manifesting itself in the morphological changes of erythrocytes and their electrophoretic mobility.…”

Section: Resultsmentioning

confidence: 99%

Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Deryugina¹,

Ivashchenko²,

Ignatiev³

et al. 2019

Sovrem Tehnol Med

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The aim of the investigation was to study a phase portrait, electrophoretic mobility of erythrocytes (EPME), and clinical and laboratory blood indices in patients with various types of diseases. Materials and Methods. A phase portrait, EPME, and clinical and laboratory blood indices have been studied in patients with cardiovascular, bronchopulmonary, gynecological, and gastroenterological diseases. The phase portrait was investigated using laser interferometry technique, EPME was determined by microelectrophoresis. The clinical and laboratory indices included counting the number of erythrocytes and leukocytes, measurements of hemoglobin content and ESR by means of standard clinical methods. The phase portrait and EPME were studied under the action of adrenaline and cortisol in experiments in vitro. Results. Using laser interferometry, phase images of erythrocytes in cardiovascular, gastroenterological, bronchopulmonary, and gynecological diseases have been obtained. The analysis of the gallery of erythrocyte phase images has shown that alterations were unidirectional in all types of pathologies and were characterized by the increase of echinocyte number. The morphological modification of erythrocytes correlated with EPME changes and clinical and laboratory blood indices and reflected the degree of stress and triggering of adaptive processes in the course of the conducted therapy. Influence of stress-realizing systems has been confirmed by the in vitro experiments under the action of adrenaline and cortisol. Conclusion. Indices of EPME and erythrocyte phase portrait obtained with the help of the current techniques of cellular microelectrophoresis and laser interferometry provide the opportunity to obtain new information on the internal structure of cytoobjects without special preparations of specimens and contrast or fluorescent probes. These indices may be applied for quantitative characteristic of the stress reaction degree and the development of adaptive processes which is of great importance for the transition to personified medicine.

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“…Também apresenta efeitos anti-inflamatórios, vasodilatação, melhora da deformabilidade eritrocitária, resultando em melhor suprimento de oxigênio, diminuindo o dióxido de carbono; melhora da hipóxia e normalização do tecido com aumento da síntese de ATP; efeito na liberação de óxido nítrico de monócitos. Tem mostrado reduzir o conteúdo de proteína C reativa, aumenta a atividade do complemento e o nível de imunoglobulina plasmática (IgA, IgM, IgG), além de diminuir a capacidade de agregação de trombócitos e ativa a fibrinólise que aumenta a circulação periférica (Weber et al, 2007;Kazemikoo et al, 2013;Tomimura et al, 2014;Deryugina et al, 2019). Hoje, o que promovemos são as abordagens transcutânea e transmucosa (Lizarelli et al, 2021), ou seja, não introduzimos um cateter dentro do vaso sanguíneo, porém entregamos a mesma energia necessária, corrigindo a potência e a irradiância entregue na pele, onde o vaso calibroso de interesse se aproxima mais da superfície.…”

Section: Introductionunclassified

Associação de diferentes abordagens da laserterapia de baixa potência no tratamento da deiscência cirúrgica de artroplastia do joelho

Freitas

Minicucci

Bragnato

et al. 2021

RSD

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O objetivo do trabalho foi demonstrar a associação da fotobiomodulação, terapia fotodinâmica, terapia a laser a vácuo, irradiação de sangue com laser transcutâneo e curativo gel de Polihexametileno Biguanida tópicos no tratamento de deiscências de artroplastia de joelho. A metodologia utilizada foi um relato de caso descritivo, com coleta de dados do tratamento da paciente por meio do prontuário eletrônico. Foram analisados os sinais e sintomas, apresentados pela paciente, bem como as características da ferida. Dessa forma, os protocolos utilizados foram prescritos de forma individualizada, garantindo um atendimento diferenciado para a paciente. Foi autorizado pelo comitê de ética e a paciente assinou o termo de consentimento livre e esclarecido. Os resultados demostraram que a associação de diferentes abordagens da fotobiomodulação foram eficazes na cicatrização completa da ferida. Após 8 dias, observamos uma diminuição da profundidade do leito, do eritema e da inflamação do tecido ao redor com delimitação das bordas da ferida; bem como o clareamento da pele. Por tratar-se de uma ferida crônica, infectada a cicatrização completa aconteceu em sessenta e três dias. Conclui-se que que a combinação da ação fotodinâmica com pressão negativa estimulando a vascularização e a oxigenação bem como a melhora das condições corporais com SVPBM, promoveu uma recuperação muito mais rápida do que cada ação individual.

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Low-level laser therapy as a modifier of erythrocytes morphokinetic parameters in hyperadrenalinemia

Cited by 15 publications

References 43 publications

Effect of Radiotherapy and Low-Level Laser Therapy on Circulating Blood Cells of Rats

Effect of Radiotherapy and Low-Level Laser Therapy on Circulating Blood Cells of Rats

Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Associação de diferentes abordagens da laserterapia de baixa potência no tratamento da deiscência cirúrgica de artroplastia do joelho

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