Low‐level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine achilles tendon fibroblasts <i>In Vitro</i>

Chen, Chia-Hsin; Tsai, Jin-Lian; Wang, Yan‐Hsiung; Lin, Chien-Chung; Chen, June-Kai; Huang, Mao-Hsiung

doi:10.1002/jor.20800

Cited by 54 publications

(45 citation statements)

References 32 publications

(28 reference statements)

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“…1b). Our results agreed with several previous reports, which showed low-level laser might improve tendon healing through increase of collagen synthesis [24]. Nonetheless, the trend of increased expression of type III collagen (116 % at 24 h, 145 % at 48 h) did not reach statistical significance during the first 48 h after laser treatment.…”

Section: Discussionsupporting

confidence: 82%

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Chen¹,

Huang²,

Sun

et al. 2014

Lasers Med Sci

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For decades, low-level laser therapy (LLLT) has widespread applications in tendon-related injuries. Although the therapeutic effect of LLLT could be explained by photostimulation of target tissue and cells, how tenocytes sense photonic energy and convert them into cascades of cellular and molecular events is still not well understood. This study was designed to elucidate the effects of LLLT on cell proliferation and collagen synthesis by examining the associated second messengers including ATP, Ca(2+), and nitric oxide using rat Achilles tenocytes. Moreover, proliferating cell nuclear antigen (PCNA) and transforming growth factor-β1 (TGF-β1) related to cell proliferation and matrix metabolism were also studied. The results showed that 904 nm GaAs laser of 1 J/cm(2) could significantly increase the MTT activity and collagen synthesis of tenocytes. Second messengers including ATP and intracellular Ca2+ were increased after laser treatment. Quantitative PCR analysis of tenocytes treated with laser revealed up-regulated expression of PCNA, type I collagen, and TGF-β1. Besides, laser-induced TGF-β1 expression was significantly inhibited by extracellular signal-regulated kinase (ERK) specific inhibitor (PD98059). The findings suggested that LLLT stimulated ATP production and increased intracellular calcium concentration. Directly or indirectly via production of TGF-β1, these second messengers mediated the proliferation of tenocytes and synthesis of collagen.

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Section: Discussionsupporting

confidence: 82%

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Chen¹,

Huang²,

Sun

et al. 2014

Lasers Med Sci

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“…Although the use of irradiation therapy in clinical practice has been discussed during the last years and previous data has shown the potential influence of light on the modulation of DNA synthesis and gene expression [4][5][6][7], very little is still known about the molecular pathways by which environmental exposures induces cellular responses, thus making the epigenetic mechanisms a plausible candidate for the linkage between the exposure to light and the molecular adaptive changes [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…In vitro previous studies associated the low-level laser therapy to the modulation of cellular mechanisms such as DNA synthesis and gene expression [4,5], production of fibrotic cells [6], stimulation of cell proliferation [7][8][9], and cell differentiation [10][11][12][13], thus providing evidences to support a hypothesis of the implication of epigenetic mechanisms in the molecular responses that are evoked in the cells by the exposure to light sources [14].…”

Section: Introductionmentioning

confidence: 95%

Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing

et al. 2016

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The use of light emitting diodes (LED) as a therapeutic resource for wound healing has increased over the last years; however, little is still known about the molecular pathways associated to LED exposure. In the present study, we verified the effects of LED therapy on DNA methylation and expression of the DNA methyltransferase (Dnmt) genes, Dnmt1 and Dnmt3a, in an in vivo model of epithelial wound healing. Male Wistar rats were submitted to epithelial excision in the dorsal region and subsequently distributed within the experimental groups: group 1, animals that received irradiation of 0.8 J/cm(2) of LED (604 nm); group 2, animals that received 1.6 J/cm(2) of LED (604 nm); control (CTL), animals not submitted to therapeutic intervention. LED applications were performed during 7 days, and tissues from the periphery of the wound area were obtained for molecular analysis. The Image-J software was used for analysis of the wound area. DNA methylation was evaluated by ELISA-based method and gene expressions were quantified by real-time PCR. Decrease on global DNA methylation profile was observed in all experimental groups (CTL, 1, and 2) revealing the participation of DNA methylation in the healing process. Significant decrease in the wound area accompanied by increase in the Dnmt3a expression was associated to group 2. Based on our findings, we propose that DNA methylation is an important molecular mechanism associated to wound healing and that irradiation with 1.6 J/cm(2) of LED evokes an increase in the expression of the Dnmt3a that might associates to the efficiency of the epithelial wound healing.

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“…HSP 70 is a protein generated in the body to improve the ability to resist hypoxia, ischemia, inflammation, heavy metal pollution, and endotoxin pollution [2]. Monochromatic light therapy can promote cell division and proliferation, including fibroblasts cells, keratinocyte cells, human osteoblast cells, lymphocyte cells, mesenchymal stem cells, aortic smooth muscle cells, artery cells, and vein endothelial cells [3][4][5][6][7][8][9]. It can also promote fibroblast synthesis, which can contribute to scar formation [10].…”

Section: Introductionmentioning

confidence: 98%

Study on the feasibility of LEDs in wound healing applications

Fan

Bao

et al. 2015

Appl. Opt.

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Poor wound healing is a common secondary complication for many diseases. Light therapy is a new way to improve wound healing. In this paper, we use LED to study the light therapy effect under low power and find the optimum irradiation parameters. It can be meaningful for practical application. A 630 nm LED and an 830 nm LED were used to research the influence of low-level light therapy on wound healing through cytological and animal experiments. In the cytological experiment, the cell viability and synthesis of hydroxyproline was measured. In the animal experiment, rats were given an artificial wound. Then, the effects of LED irradiation on wound healing were compared with control rats that did not receive the light therapy. The wound healing rate and collagen fiber formation was researched. This work for the first time, to our best knowledge, determines the optimal parameters for low-level light therapy by LED. The effect of LED application on wound healing was explored.

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Low‐level laser irradiation promotes cell proliferation and mRNA expression of type I collagen and decorin in porcine achilles tendon fibroblasts In Vitro

Cited by 54 publications

References 32 publications

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Second messengers mediating the proliferation and collagen synthesis of tenocytes induced by low-level laser irradiation

Effects of the led therapy on the global DNA methylation and the expression of Dnmt1 and Dnmt3a genes in a rat model of skin wound healing

Study on the feasibility of LEDs in wound healing applications

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