Low-Level Laser Irradiation (InGaAlP-660 nm) Increases Fibroblast Cell Proliferation and Reduces Cell Death in a Dose-Dependent Manner

Frigo, Lúcio; Fávero, Giovani Marino; Lima, Haroldo J. Campos; Maria, Durvanei Augusto; Bjordal, Jan Magnus; Joensen, Jón; Iversen, Vegard Vereide; Marcos, Rodrigo Labat; Parizzoto, Nivaldo Antônio; Lopes-Martins, Rodrigo Álvaro Brandão

doi:10.1089/pho.2008.2475

Cited by 55 publications

(39 citation statements)

References 39 publications

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“…We found that 16 J/cm 2 of laser irradiation did not affect RPE proliferation Nuclei were counterstained with DAPI and are shown in blue on the first day and even had a negative effect on the second day. This is similar with findings by Frigo et al [21] and AlGhamdi et al [22] in different cell types. A higher dose of LLLI would cause over-release of intracellular Ca 2+ and exhaust the ATP reserves.…”

Section: Discussionsupporting

confidence: 93%

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Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells

Dang

et al. 2015

Lasers Med Sci

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Low-level laser irradiation (LLLI) modulates a set of biological effects in many cell types such as fibroblasts, keratinocytes, and stem cells. However, no study to date has reported the effects of LLLI on retinal pigment epithelia (RPE) cells. The aim of this study was to investigate whether LLLI could enhance the proliferation of RPE cells and increase the expression of RPE functional genes/proteins. Human ARPE-19 cells were seeded overnight and treated with 8 J/cm(2) of LLLI. Cell proliferation was measured by CCK8 assay and cell cycle distribution was evaluated by FACS. The transcription of cell cycle-specific genes and RPE functional genes was quantified by RT-PCR. Moreover, the expression of ZO-1 and CRALBP were evaluated by immunostaining. A dose of 8 J/cm(2) of LLLI significantly increased proliferation and promoted cell cycle progression while upregulating the transcription of CDK4 and CCND1 and decreasing the transcription of CDKN2A, CDKN2C, and CDKN1B in human ARPE-19 cells. Additionally, LLLI enhanced the expression of ZO-1 and CRALBP in human ARPE-19 cells. In conclusion, LLLI could enhance the proliferative ability of human ARPE-19 cells by modulating cyclin D1, CDK4, and a group of cyclin-dependent kinase inhibitors. It also could increase the expression of RPE-specific proteins. Thus, LLLI may be a potential approach for the treatment of RPE degenerative diseases.

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Section: Discussionsupporting

confidence: 93%

“…Therefore, we concluded that LLLI promoted cell cycle progression by modulating cell cycle-specific proteins. However, the effects of LLLI on cell proliferation or cell cycle progression are mainly determined by cell type [21,23,24], cell metabolic rate [21], cell cycle period [21], and parameters of the laser instrument [22,25].…”

Section: Discussionmentioning

confidence: 99%

Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells

Dang

et al. 2015

Lasers Med Sci

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“…Frigo et al reported that 660-nm LPLI at 21 J/cm 2 negatively affected 3T3 murine fibroblast cells, as it increased cell death and inhibited cell proliferation (9). Our earlier study first reported that 632.8-nm LPLI at 60 J/cm 2 , which was named high-fluence, low-power laser irradiation (HF-LPLI), could induce cancer cell apoptosis, as evidenced by caspase-3 activation (35).…”

Section: Innovationmentioning

confidence: 99%

Cancer PhototherapyviaSelective Photoinactivation of Respiratory Chain Oxidase to Trigger a Fatal Superoxide Anion Burst

Zhou

Wei

et al. 2014

Antioxidants & Redox Signaling

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Aims: Here, we develop a novel cancer treatment modality using mitochondria-targeting, high-fluence, lowpower laser irradiation (HF-LPLI) in mouse tumor models and explore the mechanism of mitochondrial injury by HF-LPLI. Results: We demonstrated that the initial reaction after photon absorption was photosensitization of cytochrome c oxidase (COX), to inhibit enzymatic activity of COX in situ and cause respiratory chain superoxide anion (O 2 -) burst. We also found that HF-LPLI exerted its main tumor killing effect through mitochondrial O 2 -burst via electron transport chain (ETC). These phenomena were completely absent in the respiration-deficient cells and COX knockdown cells. With a carefully selected irradiation protocol, HF-LPLI could efficaciously destroy tumors. The inhibition of enzymatic activity of COX and generation of O 2 -by HF-LPLI in vivo were also detected. Innovation: It is the first time that the mechanism involved in the interaction between light and its photoacceptor under HF-LPLI treatment is clarified. Our results clearly indicate that HF-LPLI initiates its effects via targeted COX photoinactivation and that the tumor-killing efficacy is dependent of the subsequent mitochondrial O 2 -burst via ETC. Conclusion: Based on both in vitro and in vivo results, we conclude that HF-LPLI can selectively photoinactivate respiratory chain oxidase to trigger a fatal mitochondrial O 2 -burst, producing oxidative damage on cancer cells. This study opens up the possibilities of applications of HF-LPLI as a mitochondria-targeting cancer phototherapy. Antioxid. Redox Signal. 20,[733][734][735][736][737][738][739][740][741][742][743][744][745][746]

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“…Another study tested the effects of red laser 660nm (150 and 1050 J/cm 2 ) on fibroblasts. 14 The group that received 1050 J/cm 2 at 48 h presented a delay in cell growth and higher rates of cell death. The authors suggested a dose dependency that can be determined from the dosage used and the time of irradiation.…”

mentioning

confidence: 98%

“…In cell cultures, any changes in the irradiation protocol can induce alterations in the outcome. 14 In clinical studies of chronic tendinopathy, high doses of laser (21 J) have yielded poor results. 15 On the other hand, a study of liver regeneration analysis in rats described a stimulation effect with a red laser with 22.5 J/cm 2 of energy density.…”

mentioning

confidence: 99%

Laser Phototherapy at High Energy Densities Do Not Stimulate Pre-Osteoblast Growth and Differentiation

Pacheco

Oliveira

et al. 2013

Photomedicine and Laser Surgery

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Low-Level Laser Irradiation (InGaAlP-660 nm) Increases Fibroblast Cell Proliferation and Reduces Cell Death in a Dose-Dependent Manner

Cited by 55 publications

References 39 publications

Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells

Effects of low-level laser irradiation on proliferation and functional protein expression in human RPE cells

Cancer PhototherapyviaSelective Photoinactivation of Respiratory Chain Oxidase to Trigger a Fatal Superoxide Anion Burst

Laser Phototherapy at High Energy Densities Do Not Stimulate Pre-Osteoblast Growth and Differentiation

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