Low-level laser irradiation, cyclooxygenase-2 (COX-2) expression and necrosis of random skin flaps in rats

Esteves, Ivaldo; Masson, Igor Fagioli Bordello; Oshima, Celina T. F.; Paiotti, Ana Paula Ribeiro; Liebano, Richard Eloin; Plapler, Hélio

doi:10.1007/s10103-011-1011-1

Cited by 21 publications

(4 citation statements)

References 38 publications

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“…Several recent reports have suggested that low level red/near infrared light irradiation is an effective alternative treatment that reduces inflammation caused by periodontal disease . Many physiological studies have reported that low‐level laser treatment (LLLT) and light‐emitting diode treatment (LEDT) can reduce pain and inflammation and accelerate wound healing .…”

Section: Introductionmentioning

confidence: 99%

Modulation of Lipopolysaccharide‐Induced NF‐κB Signaling Pathway by 635 nm Irradiation via Heat Shock Protein 27 in Human Gingival Fibroblast Cells

Lim

Kim

et al. 2012

Photochem & Photobiology

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Heat shock protein-27 (HSP27) is a member of the small HSP family which has been linked to the nuclear factor-kappa B (NF-κB) signaling pathway regulating inflammatory responses. Clinical reports have suggested that low-level light therapy/laser irradiation (LLLT) could be an effective alternative treatment to relieve inflammation during bacterial infection associated with periodontal disease. However, it remains unclear how light irradiation can modulate the NF-κB signaling pathway. We examined whether or not 635 nm irradiation could lead to a modulation of the NF-kB signaling pathway in HSP27-silenced cells and analyzed the functional cross-talk between these factors in NF-κB activation. The results showed that 635 nm irradiation led to a decrease in the HSP27 phosphorylation, reactive oxygen species (ROS) generation, I-κB kinase (IKK)/inhibitor of κB (IκB)/NF-κB phosphorylation, NF-κB p65 translocation and a subsequent decrease in the COX-1/2 expression and prostaglandin (PGE(2) ) release in lipopolysaccharide(LPS)-induced human gingival fibroblast cells (hGFs). However, in HSP27-silenced hGFs, no obvious changes were observed in ROS generation, IKK/IκB/NF-κB phosphorylation, NF-κB p65 translocation, nor in COX-1/2 expression, or PGE(2) release. This could be a mechanism by which 635 nm irradiation modulates LPS-induced NF-κB signaling pathway via HSP27 in inflammation. Thus, HSP27 may play a role in regulating the anti-inflammatory response of LLLT.

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Section: Introductionmentioning

confidence: 99%

Modulation of Lipopolysaccharide‐Induced NF‐κB Signaling Pathway by 635 nm Irradiation via Heat Shock Protein 27 in Human Gingival Fibroblast Cells

Lim

Kim

et al. 2012

Photochem & Photobiology

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“…Researchers have focused on enhancing flap viability to avoid potential necrosis of the flaps [123]. Various pharmacological and surgical delay procedures may be used for enhancement of skin flap viability [3].…”

mentioning

confidence: 99%

“…Random skin flaps have been widely used for loss of skin and subcutaneous tissues for thousands of years [ 1 ]. Unlike the axial pattern flap, the blood supply of a random pattern flap is based on small unnamed vessels.…”

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confidence: 99%

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Effects of NecroX-5 on the Survival of a Random Pattern Skin Flap in Mice

Kang

Kim

et al. 2015

Arch Plast Surg

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Low-level laser therapy as an adjunct to conventional therapy in the treatment of diabetic foot ulcers

et al. 2016

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Foot ulcers are serious complications of diabetes mellitus (DM) and are known to be resistant to conventional treatment. This study was conducted to evaluate the efficacy of low-level laser therapy (LLLT) for the treatment of diabetic foot ulcers in a tertiary care centre (Department of Surgery, Mahatma Gandhi Memorial Medical College and Maharaja Yashwantrao Hospital, A.B. Road, Indore). A total of 30 patients with type 2 DM having Meggitt-Wagner grade I foot ulcers of more than 6 weeks duration with negative culture were studied. Patients were randomized into two groups of 15 each. Patients in study group received LLLT (660 ± 20 nm, 3 J/cm) along with conventional therapy and those in control group were treated with conventional therapy alone. The primary outcome measure was the absolute and relative wound size reduction at 2 weeks compared to the baseline parameter. Percentage ulcer area reduction was 37 ± 9% in the LLLT group and 15 ± 5.4% in the control group (p < 0.001). For ∼75% of wounds of the treatment group, wound area reduction of 30-50% was observed. In contrast, for the control group, ∼80% of wounds showed a wound area reduction of <20% on day 15. Further, the wounds with initial wound area 1000-2000 mm seems to have better final outcome than the groups with larger areas. The treated groups showed higher amount of granulation than the control group. The results suggest that LLLT is beneficial as an adjunct to conventional therapy in the treatment of diabetic foot ulcers.

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Low-level laser irradiation, cyclooxygenase-2 (COX-2) expression and necrosis of random skin flaps in rats

Cited by 21 publications

References 38 publications

Modulation of Lipopolysaccharide‐Induced NF‐κB Signaling Pathway by 635 nm Irradiation via Heat Shock Protein 27 in Human Gingival Fibroblast Cells

Modulation of Lipopolysaccharide‐Induced NF‐κB Signaling Pathway by 635 nm Irradiation via Heat Shock Protein 27 in Human Gingival Fibroblast Cells

Effects of NecroX-5 on the Survival of a Random Pattern Skin Flap in Mice

Low-level laser therapy as an adjunct to conventional therapy in the treatment of diabetic foot ulcers

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