Low‐level laser and adipose‐derived stem cells altered remodelling genes expression and improved collagen reorganization during tendon repair

Lucke, Letícia D.; Bortolazzo, Fernanda Oriani; Theodoro, Viviane; Fujii, Lucas de Oliveira; Bombeiro, André Luís; Felonato, Maíra; Dalia, Rodrigo Augusto; Carneiro, Giane Daniela; Cartarozzi, Luciana Politti; Vicente, Cristina Pontes; Oliveira, Alexandre Leite Rodrigues de; Mendonça, Fernanda Aparecida Sampaio; Esquisatto, Marcelo Augusto Marretto; Pimentel, Edson Rosa; Aro, Andréa Aparecida de

doi:10.1111/cpr.12580

Cited by 20 publications

(22 citation statements)

References 40 publications

(146 reference statements)

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“…As it is reported, a low-level laser in combination with adipose-derived mesenchymal stem cells improves the initial phase of tendon repair. 154 Based on the investigation by Badawy et al, laser therapy can be used to recover hand flexor tendon repair. 155 Carpal Tunnel Syndrome Tezcan et al investigated the effect of LLLT on 34 patients with carpal tunnel syndrome.…”

Section: Tendon Repairmentioning

confidence: 99%

Evaluation of Efficacy of Low-Level Laser Therapy

et al. 2020

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Introduction: Given the inconsistencies in the literature regarding laser performance in nonsurgical treatments, this study investigated the available literature to determine the advantages and disadvantages of low-power lasers in treating non-surgical complications and diseases. Methods: Authentic information from articles was extracted and evaluated to assess low-power laser performance for non-surgical treatments. A systematic search of studies on low-level laser therapy (LLLT) for non-surgical treatments was conducted mainly in PubMed and google scholar articles. Results: Four categories of diseases, including brain-related diseases, skin-related diseases, cancers, and bone-related disorders, which were treated by LLLT were identified and introduced. The various types of LLLT regarding the studied diseases were discussed. Conclusion:Positive aspects of LLLT versus a few disadvantages of its application imply more investigation to find better and efficient new methods.

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Section: Tendon Repairmentioning

confidence: 99%

Evaluation of Efficacy of Low-Level Laser Therapy

et al. 2020

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“…In this study, no difference was observed in the amount of TNFa cytokines among the groups. 28 The GaAlAs laser (650 nm, 4 J/cm 2 ) improved the repair in mouse model of skin photoaging by increasing the proliferation, differentiation, and secretion of the growth factor of the MSCs. Furthermore, the expression of the mesenchymal surface markers increased.…”

Section: The Variety Of Mesenchymal Stem Cells Umbilical Cord-mesenchmentioning

confidence: 99%

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

et al. 2019

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Introduction: The purpose of this study is to investigate the effect of a low-power laser on the proliferation, migration, differentiation of different types of mesenchymal stem cells (MSCs) in different studies. Methods: The relevant articles that were published from 2004 to 2019 were collected from the sources of PubMed, Scopus, and only the articles specifically examining the effect of a lowpower laser on the proliferation, differentiation, and migration of the MSCs were investigated. Results: After reviewing the literature, only 42 articles were found relevant. Generally, most of the studies demonstrated that different laser parameters increased the proliferation, migration, and differentiation of the MSCs, except the results of two studies which were contradictory. In fact, changing the parameters of a low-power laser would affect the results. On the other hand, the source of the stem cells was reported as a key factor. In addition, the combination of lasers with other therapeutic approaches was found to be more effective. Conclusion: The different parameters of lasers has been found to be effective in the proliferation, differentiation, and migration of the MSCs and in general, a low-power laser has a positive effect on the MSCs, helping to improve different disease models.

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“…21st days, 3,5,24,74 with involvement of other genes such as LOX, DCN (Decorin), MMP2, TGFb1, and Tnmd (Tenomodulin).…”

Section: Discussionmentioning

confidence: 99%

“…A transverse partial transection (approximately 2/3 of the extent of the injury in-depth) was performed in the proximal tendon region located at a distance of 4 mm from its insertion in the calcaneus bone. 3,5,24,37 Approximately 4.5 × 10 5 ASC (6/7P) were resuspended in 30 μL of DMPBS Flush and transplanted in the TR of tendons in the C and MC groups, using a pipette. In tendons of T and M groups, only 30 μL of DMPBS Flush was applied.…”

Section: Achilles Tendon and Application Of Asc And/or Microcurrentmentioning

confidence: 99%

Microcurrent and adipose‐derived stem cells modulate genes expression involved in the structural recovery of transected tendon of rats

et al. 2020

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Tendon injuries are common and have a high incidence of re-rupture that can cause loss of functionality. Therapies with adipose-derived stem cells (ASC) and the microcurrent (low-intensity electrical stimulation) application present promising effects on the tissue repair. We analyzed the expression of genes and the participation of some molecules potentially involved in the structural recovery of the Achilles tendon of rats, in response to the application of both therapies, isolated and combined. The tendons were distributed in five groups: normal (N), transected (T), transected and ASC (C) or microcurrent (M) or with ASC, and microcurrent (MC). Microcurrent therapy was beneficial for tendon repair, as it was observed a statistically significant increase in the organization of the collagen fibers, with involvement of the TNC, CTGF, FN, FMDO, and COL3A1 genes as well as PCNA, IL-10, and TNF-α. ASC therapy significantly increased the TNC and FMDO genes expression with no changes in the molecular organization of collagen. With the association of therapies, a significant greater collagen fibers organization was observed with involvement of the FMOD gene. The therapies did not affect the expression of COL1A1, SMAD2, SMAD3, MKX, and EGR1 genes, nor did they influence the amount of collagen I and III, caspase-3, tenomodulin (Tnmd), and hydroxyproline. In conclusion, the application of the microcurrent isolated or associated with ASC increased the organization of the collagen fibers, which can result in a greater biomechanical resistance in relation to the tendons treated only with ASC. Future studies will be needed to demonstrate the biological effects of these therapies on the functional recovery of injured tendons.

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Low‐level laser and adipose‐derived stem cells altered remodelling genes expression and improved collagen reorganization during tendon repair

Cited by 20 publications

References 40 publications

Evaluation of Efficacy of Low-Level Laser Therapy

Evaluation of Efficacy of Low-Level Laser Therapy

The Effect of Photobiomodulation Therapy on the Differentiation, Proliferation, and Migration of the Mesenchymal Stem Cell: A Review

Microcurrent and adipose‐derived stem cells modulate genes expression involved in the structural recovery of transected tendon of rats

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