Low Level Incorporation of Tritiated Thymidine Into the Nuclear Dna of Purkinje Neurons of Adult Mice

Cameron, I. L.; Pool, Mary R. H.; Hoage, Terrell R.

doi:10.1111/j.1365-2184.1979.tb00167.x

Cited by 4 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In neoplastic and normal cells undergoing mitosis, cytotoxicity is effected by interference with deoxyribonucleic acid (DNA) synthesis through inhibition of D N A polymerase activity 1121. Although Purkinje cells are postmitotic, some synthesis of nuclear DNA persists (in adult mice), possibly for gene amplification, turnover, or repair [9]. In rabbits, systemically administered Ara-C is taken up and phosphorylated to its active metabolite by brain cells [37].…”

Section: Discussionmentioning

confidence: 99%

Cerebellar degeneration caused by high‐dose cytosine arabinoside: A clinicopathological study

Winkelman

Hines

1983

Annals of Neurology

136

View full text Add to dashboard Cite

Twenty-four patients with leukemia or lymphoma refractory to conventional chemotherapy were given a course of systemic, high-dose cytosine arabinoside (3 gm/m2 every 12 hours for twelve doses). Four patients developed cerebellar degeneration during treatment. Ataxia of gait and limb movements, dysarthria, and nystagmus appeared five to seven days after the first dose, worsened over the next two to three days, and then remained stable for two to six days. Incomplete improvement occurred over the following one to two weeks. Postmortem examination disclosed loss of Purkinje cells in the depths of cortical sulci with relative preservation of those at the crests of folia and those in the most posterior inferior portions of the cerebellum. Other patients developed a mild, reversible cerebellar syndrome over the same time course as that of the irreversible disorder. Manifestations ranged from nystagmus alone to dysarthria and unsteadiness of gait without limb ataxia. We conclude that cytosine arabinoside at this dosage causes a cerebellar degeneration with characteristic clinical and pathological features. Among the present patients with refractory hematological malignancies, such degeneration occurred with an incidence of 16.7%, more than twice that reported in previous series.

show abstract

Section: Discussionmentioning

confidence: 99%

Cerebellar degeneration caused by high‐dose cytosine arabinoside: A clinicopathological study

Winkelman

Hines

1983

Annals of Neurology

136

View full text Add to dashboard Cite

show abstract

“…Cerebral endothelial cells and pericytes undergo mitosis (Marei & Lodin, 1974) which may determine, in part, their susceptibility to infection. DNA synthesis, as determined by thymidine autoradiography, is usually significantly restricted in differentiated neurons (Cameron et al, 1979). The delayed appearance of virus budding in neurons might be explained by the initiation of DNA repair in damaged neurons allowing productive viral infection late in the disease.…”

Section: Discussionmentioning

confidence: 99%

Spongiform Polioencephalomyelopathy Caused by a Murine Retrovirus. Ii. Ultrastructural Localization of Virus Replication and Spongiform Changes in the Central Nervous System

Swarz

Brooks

Johnson

1981

Neuropathology Appl Neurobio

View full text Add to dashboard Cite

The development of murine retrovirus induced spongiform polioencephalomyelopathy was studied sequentially by electron microscopy. During the initial 30 days, viral infection of the central nervous system, as evidenced by viral budding from membranes, was limited to the endothelial cells and pericytes. Viral particles were observed in the lumen of blood vessels, extracellular spaces and astrocytic endfeet surrounding blood vessels, but no morphological evidence of productive infection was found in astrocytes or neurons during early development of vacuolation. The earliest lesions in the neuropil consisted of swelling of astroglia followed by vacuolation, initially in axons and dendrites and later in neuronal and astrocytic soma, where vacuoles appeared to arise from dilated cisternae of the Golgi apparatus. Vacuoles contained only amorphous debris and fragments of membranes. Virions budding aberrantly into vacuoles were seen only in mice surviving beyond 35 days. Numerous reactive astrocytes were observed, but inflammatory cells were absent. The ultrastructural changes were remarkably similar to those described in scrapie, Kuru, and Creutzfeldt-Jakob disease.

show abstract

Deoxyribonucleic acid turnover in immature neurons of the rat cerebral cortex

Hobi

Kuenzle

1985

Neuroscience Letters

View full text Add to dashboard Cite

Low Level Incorporation of Tritiated Thymidine Into the Nuclear Dna of Purkinje Neurons of Adult Mice

Cited by 4 publications

References 4 publications

Cerebellar degeneration caused by high‐dose cytosine arabinoside: A clinicopathological study

Cerebellar degeneration caused by high‐dose cytosine arabinoside: A clinicopathological study

Spongiform Polioencephalomyelopathy Caused by a Murine Retrovirus. Ii. Ultrastructural Localization of Virus Replication and Spongiform Changes in the Central Nervous System

Deoxyribonucleic acid turnover in immature neurons of the rat cerebral cortex

Contact Info

Product

Resources

About