Low-level fluoride exposure reduces glycemia in NOD mice

Malvezzi, Maria Aparecida Pereira Nunes; Pereira, Hugo Juarez Vieira; Dionízio, Aline; Araújo, Tamara Teodoro; Buzalaf, Nathalia Rabelo; Sabino-Arias, Isabela Tomazini; Fernandes, Marcelo Adorna; Grizzo, Larissa Tercília; Magalhães, Ana Carolina; Buzalaf, Marília Afonso Rabelo

doi:10.1016/j.ecoenv.2018.10.055

Cited by 10 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent studies from our research group employing proteomic tools reported that different doses of F alter the expression of several mitochondrial proteins. Both in liver, muscle and intestine of rats and mice, exposure to F provoked alterations in energy metabolism, mitochondrial dysfunction and oxidative stress (Kobayashi et al, 2009;Lima Leite et al, 2014;Lobo et al, 2015;Pereira et al, 2016;Dionizio, A. S. et al, 2018;Malvezzi et al, 2018;Pereira et al, 2018). Similar findings were reported by Lima Leite et al (2014).…”

Section: Introductionsupporting

confidence: 69%

“…Among these are cell cycle, ion transport, apoptosis, signaling pathways and metabolism of free radicals. These effects have been described in several organs and tissues such as liver, kidney, heart, brain, muscle and intestine (Whitford, 1996;Chlubek et al, 2003;Shanthakumari et al, 2004;Strunecka, 2007;Strunecka et al, 2007;Xiong et al, 2007;Blaszczyk et al, 2008;Chouhan e Flora, 2008;Kobayashi et al, 2009;Barbier et al, 2010;Inkielewicz-Stepniak e Czarnowski, 2010;Dey et al, 2011;Pereira et al, 2013;Lima Leite et al, 2014;Pereira et al, 2016;Melo et al, 2017;Dionizio, A. et al, 2018;Dionizio, A. S. et al, 2018;Malvezzi et al, 2018;Pereira et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Proteomic analysis of mitochondria from rat liver exposed to chronic fluoride exposure in two experimental periods

Araújo¹

View full text Add to dashboard Cite

Agradeço a Deus por permitir que mais uma etapa possa ser concluída com muita dignidade, paz de espírito e sabedoria. Obrigada por permitir que eu passasse com muita saúde por todos estes momentos até hoje, sou grata pela vida que tenho, pela família, casa, meus animais, namorado, amigos e profissão. Sou grata senhor por me conceder tamanha sabedoria, paciência e força necessária para acordar todos os dias feliz e em paz para tentar fazer um dia melhor para mim e para as pessoas que estão ao meu redor, gratidão. Aos meus pais que nunca mediram esforços para me criar com muita dignidade, caráter e humildade, obrigada por terem se sacrificado tanto pagando meus estudos, comprando meus materiais, por pagarem meu transporte. Obrigado por me darem a oportunidade de crescer em um lar cheio de paz, amor, respeito, perseverança e persistência. Com isso levo para minha vida como é que uma família deve ser mediante as alegrias e as dificuldades, cheia de amor, respeito, gratidão e com muitos animais ne?!! Hoje estamos aqui, formando a primeira mestre da família, e quem diria que seria a filha de vocês não é mesmo?! Mas como sempre digo, Deus sabe de todas as coisas em nossas vidas e seu propósito é grande. Saibam que serei eternamente grata a vocês, dedico essa e todas as fases da minha vida á vocês. Muito obrigada, amo vocês. Ao meu namorado Junior Você sabe o quão Deus tem sido bom com a gente né?! Como eu poderia imaginar que aquele rapaz andando de moto na rua que eu mexi, hoje viraria parte de tanta coisa na minha vida. Você me completa, me acrescenta e me impulsiona todos os dias. Você é uma das pessoas mais pacientes que eu já conheci, uma das pessoas mais família, que se importa com o bemestar de todos ao seu redor. Você é uma pessoa muito iluminada, que eu tive a sorte de poder conhecer e ter você todos os dias na minha vida. Seu coração genuíno te faz um grande homem. Obrigada por sempre me oferecer uma palavra de conforto ou de incentivo todas as vezes que eu necessitava, obrigada pelo amor puro e sincero, companheirismo, respeito e paciência, você foi essencial para a conclusão desta fase, Te amo.

show abstract

Section: Introductionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of mitochondria from rat liver exposed to chronic fluoride exposure in two experimental periods

Araújo¹

View full text Add to dashboard Cite

show abstract

“…Curiously, some of these proteins, such as Parvalbumin alpha and Glyceraldehyde-3-phosphate dehydrogenase were reduced more than 2-fold in the untreated S mice (comparison SI vs. RI; Table S4). Moreover, an increase in Parvalbumin alpha was also recently described in the muscle of NOD mice treated with 50 ppm fluoride [20]. In addition, Neurofibromin (Q04690), which stimulates the GTPase activity of Ras, regulating its activity, was also increased more than 2-fold in the SII mice (Table S5), indicating the involvement of MAPK/ERK signaling pathways in the muscle events.…”

Section: Discussionmentioning

confidence: 53%

“…Wistar rats with streptozotocin-induced diabetes had increased insulin sensitivity when exposed to water containing 10 ppm F [19], while Sprague Dawley rats exposed to water containing 15 ppm F had increased insulin resistance [13]. Moreover, non-obese diabetic (NOD) mice exposed to water containing 10 ppm F had reduced plasma glucose levels when compared to their non-exposed counterparts [20,21].…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the effects of F on glucose homeostasis are not precisely known currently. While some studies have reported beneficial effects of F [18][19][20][21], others have not [13,22,23]. In addition, the effect of F on glucose homeostasis in A/J and 129P3/J mice is not known so far; however, interestingly, an epidemiological study revealed that fluoridation of drinking water was able to predict the prevalence and incidence of age-adjusted Type 2 Diabetes in 22 US states [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

We compared the parameters related to glucose homeostasis, and liver and muscle proteomes in fluorosis-susceptible (A/J; S) and fluorosis-resistant (129P3/J; R) mice in response to fluoride (F) exposure and exercise. Ninety male mice (45 R-mice and 45 S-mice) were randomized into three groups: (SI; RI) No-F, No-Exercise, (SII; RII) 50 ppm F, No-Exercise, (SIII; RIII) 50 ppm F, Exercise. Overall, mean F concentrations in the plasma and femur were significantly higher in R-mice compared with S-mice. In R-mice, exercise resulted in an increase in F accumulation in the femur. In S-mice, the mean plasma glucose level was significantly higher in Group II compared with Groups I and III. There was an increase in liver proteins involved in energy flux and antioxidant enzymes in non-exercise groups (I, II) of S-mice in comparison with the corresponding groups of R-mice. The results also showed a decrease in muscle protein expression in Group I S-mice compared with their R-mice counterparts. In conclusion, the findings suggest an increased state of oxidative stress in fluorosis-susceptible mice that might be exacerbated by the treatment with F. In addition, fluorosis-susceptible mice have plasma glucose levels higher than fluorosis-resistant mice on exposure to F, and this is not affected by exercise.

show abstract

Adaptive responses of the ileum of NOD mice to low‐dose fluoride: A proteomic exploratory study

Trevizol,

Buzalaf,

Dionizio

et al. 2024

Cell Biochemistry & Function

Self Cite

View full text Add to dashboard Cite

Fluoride (F) has been employed worldwide to control dental caries. More recently, it has been suggested that the consumption of low doses of F in the drinking water may reduce blood glucose levels, introducing a new perspective for the use of F for the management of blood glucose. However, the exact mechanism by which F affects blood glucose levels remains largely unexplored. Given that the small gut plays a pivotal role in glucose homeostasis, the aim of this study was to investigate the proteomic changes induced by low doses of F in the ileum of female nonobese‐diabetic (NOD) mice. Forty‐two female NOD mice were divided into two groups based on the F concentration in their drinking water for 14 weeks: 0 (control) or 10 mgF/L. At the end of the experimental period, the ileum was collected for proteomic and Western blot analyses. Proteomic analysis indicated an increase in isoforms of actin, gastrotropin, several H2B histones, and enzymes involved in antioxidant processes, as well as a decrease in enzymes essential for energy metabolism. In summary, our data indicates an adaptive response of organism to preserve protein synthesis in the ileum, despite significant alterations in energy metabolism typically induced by F, therefore highlighting the safety of controlled fluoridation in water supplies.

show abstract

Low-level fluoride exposure reduces glycemia in NOD mice

Cited by 10 publications

References 38 publications

Proteomic analysis of mitochondria from rat liver exposed to chronic fluoride exposure in two experimental periods

Proteomic analysis of mitochondria from rat liver exposed to chronic fluoride exposure in two experimental periods

Effect of Physical Exercise and Genetic Background on Glucose Homeostasis and Liver/Muscle Proteomes in Mice

Adaptive responses of the ileum of NOD mice to low‐dose fluoride: A proteomic exploratory study

Contact Info

Product

Resources

About