Low-Level Expression of MTUS1 Is Associated with Poor Survival in Patients with Lung Adenocarcinoma

Jee, Seungyun; Kim, Hyunsung; Bang, Seongsik; Kim, Yeseul; Park, Ha Young; Paik, Seung Sam; Sim, Jongsung; Jang, Kiseok

doi:10.3390/diagnostics11071250

Cited by 5 publications

(8 citation statements)

References 29 publications

(60 reference statements)

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“…To determine if coal dust can cause lung cell proliferation, we used Ki67 as an indicator of proliferation ( Jee et al, 2021 ). The cell proliferation was significantly higher in the CWP-3M group than in the CWP-1M and NS groups.…”

Section: Resultsmentioning

confidence: 99%

Inflammation and fibrosis in the coal dust-exposed lung described by confocal Raman spectroscopy

Wang

Zou

et al. 2022

PeerJ

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Background Coal workers’ pneumoconiosis (CWP) is an occupational disease that severely damages the life and health of miners. However, little is known about the molecular and cellular mechanisms changes associated with lung inflammation and fibrosis induced by coal dust. As a non-destructive technique for measuring biological tissue, confocal Raman spectroscopy provides accurate molecular fingerprints of label-free tissues and cells. Here, the progression of lung inflammation and fibrosis in a murine model of CWP was evaluated using confocal Raman spectroscopy. Methods A mouse model of CWP was constructed and biochemical analysis in lungs exposed to coal dust after 1 month (CWP-1M) and 3 months (CWP-3M) vs control tissues (NS) were used by confocal Raman spectroscopy. H&E, immunohistochemical and collagen staining were used to evaluate the histopathology alterations in the lung tissues. Results The CWP murine model was successfully constructed, and the mouse lung tissues showed progression of inflammation and fibrosis, accompanied by changes in NF-κB, p53, Bax, and Ki67. Meanwhile, significant differences in Raman bands were observed among the different groups, particularly changes at 1,248, 1,448, 1,572, and 746 cm−1. These changes were consistent with collagen, Ki67, and Bax levels in the CWP and NS groups. Conclusion Confocal Raman spectroscopy represented a novel approach to the identification of the biochemical changes in CWP lungs and provides potential biomarkers of inflammation and fibrosis.

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Section: Resultsmentioning

confidence: 99%

Inflammation and fibrosis in the coal dust-exposed lung described by confocal Raman spectroscopy

Wang

Zou

et al. 2022

PeerJ

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“…Recent studies have reported that recombinant overexpression of MTUS1 inhibited tumor cell proliferation, while reduced MTUS1 expression was associated with increased cell proliferation of oral squamous cell carcinoma cells, breast cancer cells, and ovarian cancer cells [ 9 ]. Reduced MTUS1 expression is also associated with poor prognosis in lung adenocarcinoma [ 11 ], gallbladder adenocarcinoma [ 12 ], renal cell carcinoma [ 13 ], bladder carcinoma [ 14 , 15 ], adenoid cystic carcinoma of the salivary gland [ 16 ], and squamous cell carcinoma of the tongue [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…The MTUS1 gene has transcript variants of various isoforms through alternative splicing that encode mitochondrial protein with tumor suppressor activity [ 7 , 8 ]. MTUS1 is downregulated in several types of cancers, including colorectal carcinoma [ 9 , 10 ], lung adenocarcinoma [ 11 ], gallbladder adenocarcinoma [ 12 ], renal cell carcinoma [ 13 ], bladder carcinoma [ 14 , 15 ], adenoid cystic carcinoma of salivary gland [ 16 ], squamous cell carcinoma of the tongue [ 17 ], head and neck squamous cell carcinoma [ 18 ], uveal melanoma [ 19 ], and breast carcinoma [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Low MTUS1 Protein Expression Is Associated with Poor Survival in Patients with Colorectal Adenocarcinoma

et al. 2023

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Introduction: Microtubule-associated tumor suppressor 1 (MTUS1) is a novel tumor suppressor protein involved in cell proliferation, migration, and tumor growth. MTUS1 is thought to be downregulated in various human cancers and associated with poor prognosis. We evaluated the clinicopathologic significance and prognostic value of MTUS1 in colorectal adenocarcinoma. Methods: Immunohistochemical staining for MTUS1 was performed on tissue microarrays of 393 colorectal adenocarcinoma cases, and MTUS1 staining was classified into high- and low-expression groups. Then, we investigated the correlations between MTUS1 protein expression and various clinicopathological parameters and patient survival. Results: MTUS1 protein was expressed at various grade levels in the cytoplasm of tumor cells, which showed loss or decreased expression of MTUS1. A total of 253 cases (64.4%) were classified into the low MTUS1 protein expression group and 140 cases (35.6%) into the high MTUS1 expression group. A low level of MTUS1 protein significantly correlated with tumor size (p = 0.047), histological grade (p < 0.001), lymphovascular invasion (p < 0.001), perineural invasion (p = 0.047), and lymph node metastasis (p < 0.001). Survival analyses showed that patients with low MTUS1 protein expression had worse overall survival (p = 0.007, log-rank test) and worse recurrence-free survival (p = 0.019, log-rank test) than those with high MTUS1 expression. Conclusions: Low MTUS1 protein expression is associated with adverse clinicopathological characteristics and poor survival outcomes in patients with colorectal adenocarcinoma. These results suggest that MTUS1 functions as a tumor suppressor in colorectal adenocarcinoma and could be a potential prognostic biomarker.

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“…Logistic regression showed that the expression of MTUS1 in CRC was associated with advanced pathological stages and N stages, suggesting that MTUS1 is important for tumour invasion and lymph node MTUS1 can also regulate cell division by disturbing the microtubule cytoskeleton and can also be used to predict the treatment response to paclitaxel-based chemotherapy in breast cancer [21][22][23]. In lung adenocarcinoma tissues, low MTUS1 expression is related to various clinical-pathological parameters, such as tumour size, Ki-67 proliferation index, lymphovascular invasion, and lymph node metastasis, which lead to the correspondingly poor prognosis of patients [14]. Moreover, MTUS1 expression levels can be synergistically inhibited by miR-19a and miR-19b, thereby contributing to lung cancer cell proliferation and migration [24].…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have also suggested that MTUSI is downregulated in multiple cancers, including CRC. MTUS1 is also associated with poor prognosis in patients with lung adenocarcinoma, gastric cancer, renal cell carcinoma, gallbladder carcinoma, salivary adenoid cystic carcinoma, and oral tongue squamous cell carcinoma [10,[14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

MTUS1 is a promising diagnostic and prognostic biomarker for colorectal cancer

et al. 2022

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Background The morbidity and mortality of colorectal cancer (CRC) remain high, posing a serious threat to human life and health. The early diagnosis and prognostic evaluation of CRC are two major challenges in clinical practice. MTUS1 is considered a tumour suppressor and can play an important role in inhibiting cell proliferation, migration, and tumour growth. Moreover, the expression of MTUS1 is decreased in different human cancers, including CRC. However, the biological functions and molecular mechanisms of MTUS1 in CRC remain unclear. Methods In the present study, data from The Cancer Genome Atlas (TCGA) database were analysed using R statistical software (version 3.6.3.) to evaluate the expression of MTUS1 in tumour tissues and adjacent normal tissues using public databases such as the TIMER and Oncomine databases. Then, 38 clinical samples were collected, and qPCR was performed to verify MTUS1 expression. We also investigated the relationship between MTUS1 expression and clinicopathological characteristics and elucidated the diagnostic and prognostic value of MTUS1 in CRC. In addition, the correlation between MTUS1 expression and immune infiltration levels was identified using the TIMER and GEPIA databases. Furthermore, we constructed and analysed a PPI network and coexpression modules of MTUS1 to explore its molecular functions and mechanisms. Results CRC tissues exhibited lower levels of MTUS1 than normal tissues. The logistic regression analysis indicated that the expression of MTUS1 was associated with N stage, TNM stage, and neoplasm type. Moreover, CRC patients with low MTUS1 expression had poor overall survival (OS). Multivariate analysis revealed that the downregulation of MTUS1 was an independent prognostic factor and was correlated with poor OS in CRC patients. MTUS1 expression had good diagnostic value based on ROC analysis. Furthermore, we identified a group of potential MTUS1-interacting proteins and coexpressed genes. GO and KEGG enrichment analyses showed that MTUS1 was involved in multiple cancer-related signalling pathways. Moreover, the expression of MTUS1 was significantly related to the infiltration levels of multiple cells. Finally, MTUS1 expression was strongly correlated with various immune marker sets. Conclusions Our results indicated that MTUS1 is a promising biomarker for predicting the diagnosis and prognosis of CRC patients. MTUS1 can also become a new molecular target for tumour immunotherapy.

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Low-Level Expression of MTUS1 Is Associated with Poor Survival in Patients with Lung Adenocarcinoma

Cited by 5 publications

References 29 publications

Inflammation and fibrosis in the coal dust-exposed lung described by confocal Raman spectroscopy

Inflammation and fibrosis in the coal dust-exposed lung described by confocal Raman spectroscopy

Low MTUS1 Protein Expression Is Associated with Poor Survival in Patients with Colorectal Adenocarcinoma

MTUS1 is a promising diagnostic and prognostic biomarker for colorectal cancer

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