Low level deltamethrin resistance in ticks from cattle of Kerala, a south Indian state

Jyothimol, G.; Ravindran, Reghu; Juliet, Sanis; Ajithkumar, Karapparambu Gopalan; Suresh, N.; Vimalkumar, M B; Lenka, Dibya Ranjan; Varghese, Siby; Ghosh, Srikant

doi:10.1016/j.vetpar.2014.04.028

Cited by 32 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the homozygous mutant genotype (A/A) was significantly correlated with survival rates under different doses of cypermethrin and deltamethrin [ 16 , 31 ]. However, this mutation was not detected in ticks from Guigang to Beihai in this study, consistent with previous findings in southern India [ 34 , 35 ].…”

Section: Discussionsupporting

confidence: 92%

Molecular assessment of voltage-gated sodium channel (VGSC) gene mutations in Rhipicephalus microplus from Guangxi, China

Jiang,

Xie,

et al. 2024

Parasites Vectors

View full text Add to dashboard Cite

Abstract Background Pyrethroid chemicals are one of the main acaricides used against ticks. Resistance to these chemicals has been reported to be associated with mutations in the voltage-gated sodium channel (VGSC) gene of the Rhipicephalus microplus. This study investigates R. microplus resistance to pyrethroids in Guangxi region of China, marking one of the first research efforts in this area. The findings are intended to provide vital baseline for the effective implementation of localized tick control strategies. Methods From March to July 2021, 447 R. microplus tick samples were collected from five prefecture-level cities in Guangxi. Allele-specific polymerase chain reaction (AS-PCR) was used to amplify segments C190A and G215T of the domain II S4-5 linker and T2134A of domain III S6 in the VGSC, to detect nucleotide mutations associated with resistance to pyrethroid acaricides. Subsequent analyses were conducted to ascertain the prevalence, types of mutations, and genotypic distributions within the sampled populations. Results Mutations within VGSC gene were identified across all five studied populations of R. microplus, although the mutation rates remained generally low. Specifically, the most prevalent mutation was C190A, observed in 4.9% of the samples (22/447), followed by G215T at 4.0% (18/447), and T2134A at 1.3% (6/447). The distribution of mutations across three critical sites of the VGSC gene revealed four distinct mutation types: C190A, G215T, C190A + G215T, and T2134A. Notably, the single mutation C190A had the highest mutation frequency, accounting for 4.3%, and the C190A + G215T combination had the lowest, at only 0.7%. The analysis further identified seven genotypic combinations, with the wild-type combination C/C + G/G + T/T predominating at a frequency of 90.4%. Subsequently, the C/A + G/G + T/T combination was observed at a frequency of 4.3%, whereas the C/C + T/T + T/T combination exhibited the lowest frequency (0.2%). Additionally, no instances of simultaneous mutations at all three sites were detected. Geographical differences in mutation types were apparent. Both samples from Hechi to Chongzuo cities exhibited the same three mutation types; however, C190A was the most prevalent in Hechi, while G215T dominated in Chongzuo. In contrast, samples from Beihai to Guilin each exhibited only one mutation type: G215T occurred in 12.5% (4/32) of Beihai samples, and C190A in 7.5% (4/53) of Guilin samples. Conclusions These findings underscore the relatively low frequency of VGSC gene mutations in R. microplus associated with pyrethroid resistance in the Guangxi, China. Moreover, the variation in mutation types and genotypic distributions across different locales highlights the need for regionalized strategies in monitoring and managing pyrethroid resistance in tick populations. This molecular surveillance is crucial for informing targeted control measures and mitigating the risk of widespread resistance emergence. Graphical Abstract

show abstract

Section: Discussionsupporting

confidence: 92%

Molecular assessment of voltage-gated sodium channel (VGSC) gene mutations in Rhipicephalus microplus from Guangxi, China

Jiang,

Xie,

et al. 2024

Parasites Vectors

View full text Add to dashboard Cite

show abstract

“…Though IPM involves a number of components for sustainable management of TTBDs, but the applications of chemical acaricides is still the backbone of tick management strategies. As in other parts of the world, Indian ticks are continuously exposed to different chemicals and resistance has been reported from different agro-climatic regions mainly located in the plains (Kumar et al 2014;Sharma et al 2012;Abdullah et al 2013;Jyothimol et al 2014;Singh et al 2014;Lenka et al 2016;Dutta et al 2017;Nandi et al 2018;Shakya et al 2020). In the present study, the tick isolates of a hilly state was studied.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of acaricidal resistance status of Rhipicephalus microplus ticks from the hilly state (Uttarakhand) of India and evaluation of efficacy of a natural formulation for the management of resistant ticks

et al. 2021

View full text Add to dashboard Cite

The resistance status against deltamethrin, cypermethrin, coumaphos and ivermectin was assessed of Rhipicephalus microplus from five districts of Uttarakhand, through adult immersion test and larval packet test. The field isolates were highly resistant to deltamethrin (median resistance ratio [RR 50 ] = 9.10-29.13-fold) followed by cypermethrin (2.23-3.55). Surprisingly the isolates were susceptible to coumaphos (0.34-3.17). Emerging resistance against ivermectin (1.55-3.27) was also observed in all the isolates. Elevated levels of esterases (enzyme ratio = 2.93-5.84-fold), glutathione S-transferases (5.10-10.06) and monooxygenases (1.68-4.02) in resistant fields isolates were highly correlated (47.4-86.0%) with the resistant factor (RR 50 ) determined by bioassay. All the isolates except Uttarkashi possess mutation at the 190th position in domain II of the sodium channel gene. As a mitigation strategy an Ageratum conyzoides-based characterized natural formulation was tested against all the isolates and was found effective at the concentration of 10.1-11.5%. The possibility of using the natural formulation for the management of multi-acaricide resistant ticks is discussed.

show abstract

“…Employing recently standardized discriminating concentration of commonly used anti-tick chemicals and resistant monitoring tools, the acaricide resistant status in ticks of seven states of India was worked out and found alarmingly high Sharma et al 2012;Shyma et al 2012;Singh and Rath 2014;Singh et al 2014a, b;Jyothimol et al 2014). Overall, most of the tick isolates developed resistant to multiacaricides and in many areas the efficacy of the commonly used acaricides has lost efficacy completely at optimum dose.…”

Section: Discussionmentioning

confidence: 99%

Comparative in vitro anti-tick efficacy of commercially available products and newly developed phyto-formulations against field collected and resistant tick lines of Rhipicephalus (Boophilus) microplus

et al. 2016

View full text Add to dashboard Cite

Rhipicephalus (Boophilus) microplus is considered as one of the most widely distributed tick species ecto-parasitizing on livestock and causes fatal diseases with significant production loss. To address the problem of controlling acaricide resistant tick infestations on animals, attention has been paid to develop eco-friendly phytoacaricides. The present study was undertaken to evaluate the comparative anti-tick activities of commercially available herbal and chemical products with chemically characterized phyto-formulations developed recently against field ticks and resistant tick lines of R. (B.) microplus. The chemical product Butox Ò Vet was found nearly passive against all the tested resistant tick lines. However, one of the commercial polyherbal product, Zerokeet Ò showed an efficacy (E%) of 41.8-75.4 % ( X ¼ 63:5 AE 7:4 %) using recommended dilution (1:2) against field ticks and resistant tick lines. However, the other commercial product, Erina Ò EP has very limited efficacy against all the tested tick. In comparison, the newly developed phyto-formulation, NBA/13/B/2 and NAC-01 conferred an E% of 82.4-91.3 % ( X ¼ 85:9 AE 2:1 %) and 62.3-94.6 % ( X ¼ 80:0 AE 8:1 %), respectively, against tested resistant ticks. Results indicated higher marketing potentiality of newly developed formulation in the existing tick problem scenario.

show abstract

Low level deltamethrin resistance in ticks from cattle of Kerala, a south Indian state

Cited by 32 publications

References 17 publications

Molecular assessment of voltage-gated sodium channel (VGSC) gene mutations in Rhipicephalus microplus from Guangxi, China

Molecular assessment of voltage-gated sodium channel (VGSC) gene mutations in Rhipicephalus microplus from Guangxi, China

Evaluation of acaricidal resistance status of Rhipicephalus microplus ticks from the hilly state (Uttarakhand) of India and evaluation of efficacy of a natural formulation for the management of resistant ticks

Comparative in vitro anti-tick efficacy of commercially available products and newly developed phyto-formulations against field collected and resistant tick lines of Rhipicephalus (Boophilus) microplus

Contact Info

Product

Resources

About