Low-Intensity Swimming Training Partially Inhibits Lipopolysaccharide-Induced Acute Lung Injury

Ramos, Daniel Souza; Olivo, Clarice Rosa; Lopes, Fernanda D.T.Q.S.; Toledo, Alessandra Choqueta de; Martins, Mílton A.; Osório, Rodrigo Alexis Lazo; Dolhnikoff, Marisa; Ribeiro, Wellington; Vieira, Rodolfo Paula

doi:10.1249/mss.0b013e3181ad1c72

Cited by 43 publications

(38 citation statements)

References 27 publications

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“…Furthermore, trained mice had a higher blood cell count and pathologically less cardiac, hepatic, and pulmonary injuries. In concordance with this study, decreased levels of inflammatory cells, including neutrophils and lymphocytes, were counted in the BAL fluid of trained mice [40-42]. Likewise, exercise significantly decreased IL-1β, IL-6, CXCL1, and TNF-α [40-43].…”

Section: Discussionsupporting

confidence: 62%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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Background: Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. Methods: BALB/c mice (24 weeks old) were randomized to sedentary, exercise (EX), PS, and PS + EX groups for the acute experimental setting, and PS and PS + EX groups for the chronic setting. Low-intensity AE was performed for 5 weeks, 60 min/day; 24 h after the final AE session, mice were inoculated with 5 × 10⁴ colony-forming units (CFU) of PS, and 24 h and 14 days after PS inoculation, mice were studied. Results: AE inhibited PS colonization (p < 0.001) and lung inflammation (total cells, neutrophils, lymphocytes [p < 0.01] in bronchoalveolar lavage [BAL]), with significant differences in BAL levels of IL-1β (p < 0.001), IL-6 (p < 0.01), CXCL1 (p < 0.001), and TNF-α (p < 0.001), as well as parenchymal neutrophils (p < 0.001). AE increased BAL levels of IL-10 and parenchymal (p < 0.001) and epithelial (p < 0.001) IL-10 expression, while epithelial (p < 0.001) and parenchymal (p < 0.001) NF-κB expression was decreased. AE diminished pulmonary lipid peroxidation (p < 0.001) and increased glutathione peroxidase (p < 0.01). Pre-incubation of BEAS-2B with IL-10 inhibited PS-induced epithelial cell expression of TNF-α (p < 0.05), CD40 (p < 0.01), and dichlorodihydrofluorescein diacetate (p < 0.05). Conclusions: AE inhibits PS-induced lung inflammation and bacterial colonization in elderly mice, involving IL-10/NF-κB, and redox signaling.

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Section: Discussionsupporting

confidence: 62%

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

et al. 2018

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“…2). The values were normalised to body weight because the experimental groups that were trained presented greater body mass at the end of the experimental protocol (p,0.01 compared with control and smoke groups; data not shown) [14]. We did not observe significant differences in Raw values when the four experimental groups were compared ( fig.…”

Section: Respiratory Mechanics Evaluationmentioning

confidence: 89%

“…The BAL fluid collected was centrifuged at 9006g for 8 min at 5uC, and the cell pellet was resuspended in 1 mL of physiological saline. Total cells were counted using a Neubauer haemocytometer chamber (Carl Roth, Karlsruhe, Germany) and differential cells (300 cells?slide -1 ) were evaluated by microscopic examination of BAL samples prepared in cytocentrifuge slides, stained with Diff Quick (Medion Diagnostics, Dü ndingen, Switzerland) [14].…”

Section: Total Cells Macrophages and Ros Measurement In Bronchoalveomentioning

confidence: 99%

Aerobic exercise attenuates pulmonary injury induced by exposure to cigarette smoke

Toledo¹,

Magalhães²,

Hizume³

et al. 2011

European Respiratory Journal

120

114

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It has recently been suggested that regular exercise reduces lung function decline and risk of chronic obstructive pulmonary disease (COPD) among active smokers; however, the mechanisms involved in this effect remain poorly understood.The present study evaluated the effects of regular exercise training in an experimental mouse model of chronic cigarette smoke exposure.Male C57BL/6 mice were divided into four groups (control, exercise, smoke and smoke+exercise). For 24 weeks, we measured respiratory mechanics, mean linear intercept, inflammatory cells and reactive oxygen species (ROS) in bronchoalveolar lavage (BAL) fluid, collagen deposition in alveolar walls, and the expression of antioxidant enzymes, matrix metalloproteinase 9, tissue inhibitor of metalloproteinase (TIMP)1, interleukin (IL)-10 and 8-isoprostane in alveolar walls.Exercise attenuated the decrease in pulmonary elastance (p,0.01) and the increase in mean linear intercept (p50.003) induced by cigarette smoke exposure. Exercise substantially inhibited the increase in ROS in BAL fluid and 8-isoprostane expression in lung tissue induced by cigarette smoke. In addition, exercise significantly inhibited the decreases in IL-10, TIMP1 and CuZn superoxide dismutase induced by exposure to cigarette smoke. Exercise also increased the number of cells expressing glutathione peroxidase.Our results suggest that regular aerobic physical training of moderate intensity attenuates the development of pulmonary disease induced by cigarette smoke exposure.

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“…The exhaled NO (NOex) concentrations were measured by chemiluminescence using a fastresponding analyser (NOA 280; Sievers Instruments, Boulder, CO). [25][26][27] …”

Section: Exhaled Nomentioning

confidence: 99%

Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury

Almeida

Oliveira-Júnior

Souza

et al. 2016

The Journal of Heart and Lung Transplantation

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Low-Intensity Swimming Training Partially Inhibits Lipopolysaccharide-Induced Acute Lung Injury

Abstract: These results suggest that low-intensity swimming training inhibits lung neutrophilic inflammation, but not remodeling and impaired lung mechanics, in a model of LPS-induced acute lung injury.

Cited by 43 publications

References 27 publications

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Aerobic Exercise Protects from Pseudomonas aeruginosa-Induced Pneumonia in Elderly Mice

Aerobic exercise attenuates pulmonary injury induced by exposure to cigarette smoke

Creatine supplementation attenuates pulmonary and systemic effects of lung ischemia and reperfusion injury

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