Low-intensity pulsed ultrasound regulates proliferation and differentiation of neural stem cells through notch signaling pathway

Yu, Wu; Gao, Qiang; Zhu, Shi-En; Wu, Qiuli; Zhu, Rusen; Zhong, Hao; Xing, Cong; Qu, Haodong; Wang, Dawei; Li, Bo; Ning, Guangzhi; Feng, Shiqing

doi:10.1016/j.bbrc.2020.03.142

Cited by 23 publications

(27 citation statements)

References 35 publications

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“…Tan et al [ 7 ] listed recent studies regarding the action of LIPUS on various neural stem cells and concluded that LIPUS can stimulate stem cells in vitro , promote stem cell proliferation, differentiation, and migration, and maintain stem cell activity. The findings of Wu et al [ 8 ] suggested that LIPUS regulates the Notch signalling pathway in the central nervous system, causing neural stem cell proliferation and differentiation. Additionally, LIPUS can also accelerate tissue repair[ 9 ] and promote the dissipation of inflammation[ 10 ], angiopoiesis[ 11 ], etc.…”

Section: To the Editormentioning

confidence: 99%

Physical energy-based ultrasound shifts M1 macrophage differentiation towards M2 state

Qin¹,

Luo²,

Zhu³

2022

WJSC

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Recently, we read with interest the article entitled “Unveiling the Morphogenetic Code: A New Path at the Intersection of Physical Energies and Chemical Signaling”. In this paper, the investigation into the systematic and comprehensive bio-effects of physical energies prompted us to reflect on our research. We believe that ultrasound, which possesses a special physical energy, also has a certain positive regulatory effect on macrophages, and we have already obtained some preliminary research results that support our hypothesis.

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Section: To the Editormentioning

confidence: 99%

Physical energy-based ultrasound shifts M1 macrophage differentiation towards M2 state

Qin¹,

Luo²,

Zhu³

2022

WJSC

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“…When dual antiplatelet therapy (DAPT) was applied along with LIPUS, expressions of both Notch1 and Hes1 increased leading to stem cell proliferation and differentiation. 125 Continuous low-intensity ultrasound (cLIUS) increases the expression of SOX9, a transcription factor and main regulator of chondrogenesis. The increase was associated with phosphorylation of ERK1/2, which also caused the nuclear localization of SOX9.…”

Section: Applications Of Ultrasound In Stem Cellsmentioning

confidence: 99%

“…This finding was confirmed by the decreased expression of neural markers like neuron-specific nuclear protein (NeuN) and expression of glial fibrillary acidic protein (GFAP). When dual antiplatelet therapy (DAPT) was applied along with LIPUS, expressions of both Notch1 and Hes1 increased leading to stem cell proliferation and differentiation …”

Section: Applications Of Ultrasound In Stem Cells Engineering and Reg...mentioning

confidence: 99%

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Bansal

Jha

Bhatia

et al. 2021

ACS Biomater. Sci. Eng.

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The role of ultrasound in medicine and biological sciences is expanding rapidly beyond its use in conventional diagnostic imaging. Numerous studies have reported the effects of ultrasound on cellular and tissue physiology. Advances in instrumentation and electronics have enabled successful in vivo applications of therapeutic ultrasound. Despite path breaking advances in understanding the biophysical and biological mechanisms at both microscopic and macroscopic scales, there remain substantial gaps. With the progression of research in this area, it is important to take stock of the current understanding of the field and to highlight important areas for future work. We present herein key developments in the biological applications of ultrasound especially in the context of nanoparticle delivery, drug delivery, and regenerative medicine. We conclude with a brief perspective on the current promise, limitations, and future directions for interfacing ultrasound technology with biological systems, which could provide guidance for future investigations in this interdisciplinary area.

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“…Spinal cord injury (SCI) is a traumatic disease that causes exercise, sensory, or autonomic dysfunction, thereby directly affecting the quality of life of patients and imposing a heavy financial burden on society and families (1). It is therefore crucial to clarify the pathophysiological processes associated with SCI.…”

Section: Introductionmentioning

confidence: 99%

Identification of four genes and biological characteristics associated with acute spinal cord injury in rats integrated bioinformatics analysis

Tao

et al. 2021

Ann Transl Med

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Background: Spinal cord injury (SCI) is a serious condition that can cause physical disability and sensory dysfunction. Cytokines play an extremely important role in the acute phase of SCI. Clarifying the cytokine expression profile is of great importance.Methods: Cytokine array analysis was used to explore the changes in 67 different proteins at 0 hours, 2 hours, 1 day, 3 days, and 7 days after acute SCI in rats. The differentially expressed cytokines in the various periods were analyzed and compared. The biological processes related to the differentially expressed proteins were examined using Gene Ontology (GO) analysis.Results: Immediately after SCI (0 hours), only ciliary neurotrophic factor (CNTF) was slightly upregulated, while 23 other proteins were down-regulated. At 2 hours after SCI, there were 3 upregulated and 21 downregulated proteins. At 1 day after SCI, there were 5 upregulated and 6 downregulated proteins.At 3 days after SCI, there were 6 upregulated and 4 downregulated proteins. At 7 days after SCI, there were 4 upregulated and 9 downregulated proteins. Erythropoietin (EPO) and Fms related tyrosine kinase 3 ligand (Flt-3L) were downregulated at all time points. CD48 was decreased at 2 hours to 7 days after SCI.Monocyte chemotactic protein-1 (MCP-1) was the only protein that was upregulated at 2 hours to 7 days.The GO and pathway analyses revealed that the cytokine-related pathways, cell death, and proliferation might play a key role during secondary SCI.Conclusions: This study identified 3 downregulated proteins during SCI, that being EPO, Flt-3L, and CD48. MCP-1 was the only upregulated protein, and its expression was upregulated till day 7 following SCI. These 4 identified genes may be potential therapeutic targets for the treatment of SCI.

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Low-intensity pulsed ultrasound regulates proliferation and differentiation of neural stem cells through notch signaling pathway

Cited by 23 publications

References 35 publications

Physical energy-based ultrasound shifts M1 macrophage differentiation towards M2 state

Physical energy-based ultrasound shifts M1 macrophage differentiation towards M2 state

Ultrasound-Enabled Therapeutic Delivery and Regenerative Medicine: Physical and Biological Perspectives

Identification of four genes and biological characteristics associated with acute spinal cord injury in rats integrated bioinformatics analysis

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