Low-intensity pulsed ultrasound (LIPUS) switched macrophage into M2 phenotype and mitigated necroptosis and increased HSP 70 in gentamicin-induced nephrotoxicity

Gouda, Sarah Ali Abdelhameed; Aboulhoda, Basma Emad; Abdelwahed, Omaima Mohammed; Abdallah, Hend; Rashed, Laila Ahmed; Hussein, Rania Elsayed; Sharawy, Nivin

doi:10.1016/j.lfs.2022.121338

Cited by 10 publications

(7 citation statements)

References 46 publications

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“…As for the effects of LIPUS on macrophage polarization, studies have shown that LIPUS irradiation suppresses inflammatory responses and fibrosis in drug-induced renal injury [ 29 ]. Additionally, it promotes skeletal muscle regeneration by enhancing M2-shifted macrophage polarization during the skeletal muscle repair process [ 28 , 29 ]. Therefore, it is thought that LIPUS affected macrophage polarization in the IFP, resulting in a predominance of M2 macrophages and suppression of fibrosis.…”

Section: Discussionmentioning

confidence: 99%

“…This results in a decreased release of heat shock protein and an increase in M2 macrophages. Additionally, LIPUS has been reported to increase the number of macrophages [ 29 ], suggesting a mechanism that involves the regulation of macrophage function through these effects.…”

Section: Discussionmentioning

confidence: 99%

“…The mechanism of low-intensity pulsed ultrasound (LIPUS) irradiation has also been reported to decrease macrophage infiltration into the synovial tissue and reduce inflammatory cytokines secretion [ 26 , 27 ]. Furthermore, LIPUS has been recently reported to affect macrophage polarization in muscle repair and renal disease [ 28 , 29 ]. However, it remains unclear whether LIPUS alters macrophage polarization in the IFP of KOA, potentially reducing IFP fibrosis.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Low-Intensity Pulsed Ultrasound on Macrophage Properties and Fibrosis in the Infrapatellar Fat Pad in a Carrageenan-Induced Knee Osteoarthritis Rat Model

Kitagawa,

Kawahata,

Kudo

2024

Cureus

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Background: In the progression of knee osteoarthritis (KOA), fibrosis of the infrapatellar fat pad (IFP) is a key pathological change. Low-intensity pulsed ultrasound (LIPUS) inhibits IFP fibrosis by decreasing the gene expression and activity of hypoxia-inducible factor (HIF-1α), which is a protein involved in IFP fibrosis in KOA rat models. On the other hand, macrophages play an important role in the progression of fibrosis in various tissues, and LIPUS irradiation suppresses macrophage infiltration and inflammatory cytokine secretion. However, whether LIPUS suppresses macrophage polarity and IFP fibrosis in KOA remains unclear. Therefore, we investigated the effect of LIPUS on macrophage polarity and IFP fibrosis. Materials and methods: A KOA model was created by injecting carrageenin into the bilateral knee joints of Wistar rats (eight weeks old). Tissues were harvested over time for histological and molecular biological analysis. The KOA model was also subjected to LIPUS irradiation for two weeks following the injection of carrageenin. Results: RM-4-positive cells were widely distributed in IFP two weeks after carrageenin administration, but M2 macrophages were significantly increased, and the Sirius red area was decreased in the LIPUS-irradiated group compared with those in the non-irradiated group. The gene expression of M1 macrophage markers was significantly decreased and that of M2 macrophage markers was significantly increased in the LIPUS-irradiated group. The expression of transforming growth factor-β (TGF-β) and type 1 collagen was also significantly decreased. Conclusion: These results suggest that LIPUS may serve as a novel approach for the treatment of KOA through its effect on M1 macrophages and suppression of TGF-β expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Low-Intensity Pulsed Ultrasound on Macrophage Properties and Fibrosis in the Infrapatellar Fat Pad in a Carrageenan-Induced Knee Osteoarthritis Rat Model

Kitagawa,

Kawahata,

Kudo

2024

Cureus

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“…Several recent studies note a reduction in tissue inflammation with the use of LIPUS. One potential mechanism for explaining this reductive inflammatory effect points to regulating macrophage polarization [ 137 , 141 , 142 , 143 ]. This therapeutic means may hold promise in chronic wounds where a paucity of M2 macrophages as well as impaired angiogenesis is pathophysiologically critical.…”

Section: Clinical Perspectivesmentioning

confidence: 99%

Roles of Mechanosensitive Channel Piezo1 in Wound Healing and Scar Formation

Rennekampff,

Tenenhaus,

Rennekampff

et al. 2024

Life

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The ability to heal one’s wounds is perhaps one of the most fundamental and critical of physiologic processes. This coordinated and closely regulated sequential biological process involves a variety of migratory and resident cells. The activation, modulation, balance, and control of these functions depend upon soluble mediators that activate cells and modulate their diverse functions. Recent advances have identified mechanotransduction as functionally integral in many different cell types and physiologic processes. The mechanically sensitive ion channel Pieoz1 is expressed on platelets, neutrophils, macrophages, endothelial cells, keratinocytes, and fibroblasts, all of which are principally involved in wound healing. On a cellular level, there have been great advances in our understanding of the functional role of Piezo1 mechanotransduction in cutaneous wounding. The blocking of Piezo1 has recently been shown to reduce scarring in vivo and yet, thus far, a comprehensive understanding of the roles that Piezo1 plays in in vivo wound healing remains lacking. Recognizing the ever-present and critical importance of optimal and reparative wound healing, and with the availability of new physical mechanomodulating devices, the time is ripe for gaining deeper insights into optimizing wound healing. In this review, we describe the current knowledge of Piezo1 related to wound healing.

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“…Low-intensity pulsed ultrasound is another form of mechanical stimulation that promotes repair. Several studies have shown that low-intensity pulsed ultrasound activates macrophages, enhances mRNA expression of anti-inflammatory genes (Arg-1, PPAR-γ, and IL-4), promotes M2 polarization via the WNT signalling pathway, and inhibits necroptosis via HSP-70 reduction [ 47 , 48 ]. In muscle, following pulsed ultrasound, the total macrophage population decreased but the proportion of M2-like macrophages increased [ 48 ].…”

Section: Applied Physical Stimuli Influencing Macrophage Functionmentioning

confidence: 99%

Modulation of macrophages by biophysical cues in health and beyond

Wilson

2023

Discovery Immunology

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Macrophages play a key role in tissue development and homeostasis, innate immune defence against microbes or tumours, and restoring homeostasis through tissue regeneration following infection or injury. The ability to adopt such diverse functions is due to their heterogeneous nature, which is driven largely by their developmental origin and their response to signals they encounter from the microenvironment. The most well characterised signals driving macrophage phenotype and function, are biochemical and metabolic. However, the way macrophages sense and respond to their extracellular biophysical environment is becoming increasingly recognised in the field of mechano-immunology. These biophysical cues can be signals from tissue components, such as the composition and charge of extracellular matrix or topography, elasticity, and stiffness of the tissue surrounding cells; and mechanical forces such as shear stress or stretch. Macrophages are important in determining whether disease resolves or becomes chronic. Ageing and diseases such as cancer or fibrotic disorders are associated with significant changes in the tissue biophysical environment, and this provides signals that integrate with those from biochemical and metabolic stimuli to ultimately dictate the overall function of macrophages. This review provides a brief overview of macrophage polarization, followed by a selection of commonly recognised physiological and applied biophysical stimuli impacting macrophage activity, and the potential signalling mechanisms driving downstream responses. The effects of biophysical cues on macrophages function in homeostasis and disease, and the associated clinical implications are also highlighted.

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Low-intensity pulsed ultrasound (LIPUS) switched macrophage into M2 phenotype and mitigated necroptosis and increased HSP 70 in gentamicin-induced nephrotoxicity

Cited by 10 publications

References 46 publications

Effect of Low-Intensity Pulsed Ultrasound on Macrophage Properties and Fibrosis in the Infrapatellar Fat Pad in a Carrageenan-Induced Knee Osteoarthritis Rat Model

Effect of Low-Intensity Pulsed Ultrasound on Macrophage Properties and Fibrosis in the Infrapatellar Fat Pad in a Carrageenan-Induced Knee Osteoarthritis Rat Model

Roles of Mechanosensitive Channel Piezo1 in Wound Healing and Scar Formation

Modulation of macrophages by biophysical cues in health and beyond

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