Low intensity-pulsed ultrasound induced apoptosis of human hepatocellular carcinoma cells in vitro

Shi, Mingfang; Liu, Bangzhong; Liu, Guanghua; Wang, Ping; Yang, Mingzhen; Li, Yun; Zhou, Jian

doi:10.1016/j.ultras.2015.07.011

Cited by 28 publications

(27 citation statements)

References 50 publications

(62 reference statements)

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“…As the intensity gradually increases to approximately 465 mW/cm 2 , LIPUS significantly reduced proliferation in MC3T3‐E1 cells. LIPUS also inhibits the proliferation of human hepatocellular carcinoma cells and osteosarcoma cells and promotes their apoptosis . In this study, LIPUS promoted the proliferation of BMSCs when the cells were exposed to LIPUS for 5 or 10 minutes once a day.…”

Section: Discussionmentioning

confidence: 94%

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Xie

Jiang

Wang

et al. 2019

J of Cellular Biochemistry

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Low‐intensity pulsed ultrasound (LIPUS) is a promising therapy that is widely used in clinical applications and fundamental research. Previous research has shown that LIPUS exposure has a positive effect on stem cell proliferation. However, the impact of LIPUS exposure on human bone marrow mesenchymal stem cells (hBMSCs) remains unknown. In our study, the effect and mechanism of LIPUS exposure on the proliferation of hBMSCs were investigated, and the optimal parameters of LIPUS were determined. hBMSCs were obtained and identified by flow cytometry, and the proliferation of hBMSCs was measured using the Cell Counting Kit‐8 assay to determine cell cycle and cell count. Expression levels of the phosphoinositide 3‐kinase (PI3K)/protein kinase B (AKt) pathway proteins and cyclin D1 were determined by western blot analysis. Next, hBMSCs were successfully cultured and identified as multipotent mesenchymal stem cells. We found that LIPUS could promote the proliferation of hBMSCs when the exposure time was 5 or 10 minutes per day. Furthermore, 50 or 60 mW/cm2 LIPUS had a more significant effect on cell proliferation, but if cells were irradiated by LIPUS for 20 minutes once a day, an intensity of at least 50 mW/cm2 could markedly inhibit cell growth. Cell cycle analysis demonstrated that LIPUS treatment drives cells to enter S and G2/M phases from the G0/G1 phase. LIPUS exposure increased phosphorylation of PI3K/AKt and significantly upregulated expression of cyclin D1. However, these effects were inhibited when cells were treated with PI3K inhibitor (LY294002), which in turn reduced LIPUS‐mediated proliferation of hBMSCs. These results suggest that LIPUS exposure may be involved in the proliferation of hBMSCs via activation of the PI3K/AKt signaling pathway and high expression of cyclin D1, and the intensity of 50 or 60 mW/cm2 and exposure time of 5 minutes were determined to be the optimal parameters for LIPUS exposure.

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Section: Discussionmentioning

confidence: 94%

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Xie

Jiang

Wang

et al. 2019

J of Cellular Biochemistry

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“…In response to LIPUS, cells displayed distinct effects on different cell types. LIPUS promotes proliferation of human osteoblasts or synovial cells [ 6 , 19 ], but inhibits proliferation and induces apoptosis of human hapatocellular carcinoma cells [ 27 ]. Shindo et al.…”

Section: Discussionmentioning

confidence: 99%

Low-intensity pulsed ultrasound in combination with SonoVue induces cytotoxicity of human renal glomerular endothelial cells via repression of the ERK1/2 signaling pathway

et al. 2018

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Objectives: Low-intensity pulsed ultrasound (LIPUS) and SonoVue have been used widely for diagnosis and therapeutic treatment. The effects of LIPUS and SonoVue on the microvascular system and underlying molecular mechanisms have not been established.Methods: Cultured human renal glomerular endothelial cells (HRGECs) were treated with 5-min ultrasonic irradiation, 20% SonoVue or the combination of both treatments. Cell proliferation, viablity, and apoptosis were measured by MTT assay, Trypan blue exclusion assay and flow cytometry, respectively. Activation of extracellular regulated protein kinases (ERK) were examined by Western blot.Results: We found that LIPUS and SonoVue alone do not induce cytotoxicity of HRGECs; however, the combination of the two treatments reduces cell proliferation and increases cell death. In addition, the combination of LIPUS and SonoVue suppressed the activation of ERK 1/2 in HRGRCs. With pretreatment of the inhibitor of ERK1/2 signaling, PD98059, LIPUS, and SonoVue does not induce additional cell death and inhibition of proliferation.Conclusions: LIPUS combined with SonoVue induces cytotoxicity of HRGECs via repression of the ERK1/2 signaling pathway.

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“…There have been several studies reporting that LIPUS has antitumor effects via the induction of apoptosis in cancer cells (19)(20)(21)(22)(23). For example, LIPUS was previously demonstrated to induce apoptosis by causing membrane damage in human leukemia cells (19), affecting the Ca 2+ /mitochondrial pathway in human hepatocellular carcinoma cells (23) and enhancing adjuvant chemotherapy in cases of lymphoma (20). However, there is currently limited data regarding the antitumor effects of LIPUS on osteosarcoma cells, although the treatment of osteosarcoma in children and adolescents remains a serious challenge.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

et al. 2017

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Abstract. To date, there is limited data on the biological effects of low-intensity pulsed ultrasound (LIPUS) on primary malignant bone tumors. The purpose of the present study was to investigate the antitumor effects of LIPUS on osteosarcoma cells. The effects of LIPUS on cell viability, induction of apoptosis, mitochondrial membrane potential and intracellular signaling molecules in the LM8 osteosarcoma cell line were investigated. LIPUS inhibited cell viability (P=0.0022) and mitochondrial membrane potential (P=0.0019) in LM8 cells. Flow cytometry analysis and terminal deoxynucleotidyl transferase dUTP nick end labeling staining revealed significantly higher numbers of apoptotic (P<0.0001) and necrotic cells (P=0.0091) compared with cells without treatment. LIPUS treatment significantly increased phosphorylated Akt (P<0.0001) and IκBα (P=0.0001) levels, and reduced phosphorylated mitogen-activated protein kinase 7 (P<0.0001) and phosphorylated checkpoint kinase 1 (P=0.0008) levels. These results suggest that LIPUS is a non-invasive adjuvant therapy that is able to inhibit cellular proliferation in osteosarcoma cells.

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Low intensity-pulsed ultrasound induced apoptosis of human hepatocellular carcinoma cells in vitro

Cited by 28 publications

References 50 publications

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Low‐intensity pulsed ultrasound promotes the proliferation of human bone mesenchymal stem cells by activating PI3K/AKt signaling pathways

Low-intensity pulsed ultrasound in combination with SonoVue induces cytotoxicity of human renal glomerular endothelial cells via repression of the ERK1/2 signaling pathway

Inhibitory effects of low-intensity pulsed ultrasound sonication on the proliferation of osteosarcoma cells

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