Low-Intensity Pulsed Ultrasound Enhances Early Healing of Medial Collateral Ligament Injuries in Rats

Takakura, Yoshiyuki; Matsui, Noriko; Yoshiya, Shinichi; Fujioka, Hiroyuki; Muratsu, Hirotsugu; Tsunoda, Masaya; Kurosaka, Masahiro

doi:10.7863/jum.2002.21.3.283

Cited by 55 publications

(32 citation statements)

References 13 publications

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“…Enhanced bone healing in tibial [10], radial [11], scaphoid [38] and mandibular [39] fractures has been observed. Studies carried out on animal models of ligament, tendon and cartilage injury demonstrated the beneficial effects of US stimulation in wound repair of tissues other than bone [40][41][42]. In US exposed repaired Achilles tendons, an increased order in the extracellular matrix network and collagen fibres distribution has been found [43].…”

Section: Discussionmentioning

confidence: 96%

Can ultrasound counteract bone loss? Effect of low-intensity ultrasound stimulation on a model of osteoclastic precursor

Monici¹,

Bernabei²,

Basile³

et al. 2007

Acta Astronautica

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Section: Discussionmentioning

confidence: 96%

Can ultrasound counteract bone loss? Effect of low-intensity ultrasound stimulation on a model of osteoclastic precursor

Monici¹,

Bernabei²,

Basile³

et al. 2007

Acta Astronautica

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“…61,73 Low-intensity vibro-acoustic stimulation, typically delivered in the form of pulsed US of <50 mW/cm 2 , accelerates the healing of neural tissue, 82 cartilage, 54,55 tendon, and bone. 13,14,21,23,31,33,50,57,65,72,75,85 Pulsed US treatment also increases the rate of bone formation in distractions. 11,24,25,68,79 Exposure to low-intensity pulsed US (1.5 MHz, 30 mW/cm 2 ) for 20 min a day resulted in a 36% acceleration in the healing rate of distal radial fractures 44 and a reduction in the healing time of tibia fractures from 154 to 96 days.…”

Section: Introductionmentioning

confidence: 94%

Low-Intensity Pulsed Ultrasound Modulates Shear Stress Induced PGHS-2 Expression and PGE2 Synthesis in MLO-Y4 Osteocyte-Like Cells

2010

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Fluid shear stress (SS) has been shown to be a prevailing physiological stimulus in the regulation of bone cell metabolism and so are the exogenous biomechanical forces, like ultrasound (US) and vibration. The purpose of this study is to elaborate the interplay of laminar fluid SS with low-intensity pulsed US in the regulation of prostaglandin H synthase 2 (PGHS-2) and prostaglandin E2 (PGE2). Murine long bone osteocyte-like (MLO-Y4) cells were exposed to various regimes of US (1.5 Hz, 30 mW/cm2) and SS (19 dyn/cm2) alone and sequentially. Changes in PGHS-2 gene expression levels were quantified at 3 and 24 h using real-time RT-PCR. PGE2 levels in the culture media were measured using enzyme immunoassay at 3 and 24 h. PGE2 levels significantly increased after exposure to SS for 3 and 24 h by 2.17±0.02 and 5.47±0.42-fold, respectively, compared to control cells. A 20 min US treatment prior to SS significantly increased SS PGE2 levels 2.95±0.18 and 2.90±0.50-fold at 3 and 24 h, respectively. US also significantly increased PGHS-2 mRNA levels in cells exposed to SS. SS caused a 2.74 ± 0.49-fold increase in PGHS-2 mRNA levels at 3 h and a significant 3.70±0.25-fold increase at 24 h relative to control. A 20 min US treatment caused 1.35±0.49 and 2.44±0.82-fold increase in PGHS-2 mRNA levels in cells exposed to SS at 3 and 24 h, respectively. These results indicate that combining US with SS may have a more anabolic benefit for bone tissue than either stimulus alone.

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“…Low intensity vibro-acoustic stimulation, typically delivered in the form of "diagnostic-intensity-level" pulsed ultrasound (US) of less than 50 mW/cm 2 , accelerates the healing of neural tissue, cartilage, tendon, and bone. 3,6,12,29,34,37,40 Pulsed US treatment also increases the rate of bone formation in distractions. 7 Numerous clinical studies have documented the healing of nonunions and significant reductions in the healing time of fractures that were treated with pulsed US.…”

Section: Introductionmentioning

confidence: 96%

Interdependence of Pulsed Ultrasound and Shear Stress Effects on Cell Morphology and Gene Expression

et al. 2006

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Fluid shear stress is a key biomechanical regulatory factor in a several biological systems including bone tissue. Bone cells are also regulated by exogenous acoustic vibration, which has therapeutic benefits. In this study, we determined the effects of shear stress and pulsed ultrasound (US), alone and in series on osteoblast morphology and gene expression. We observed that shear stress (19 dyne/cm(2)) elongated SaOS-2 cells at 3, 6, 24, and 48 h decreasing their shape index from control values of 0.51 +/- 0.01, 0.60 +/- 0.05, 0.59 +/- 0.04, and 0.45 +/- 0.01 to 0.45 +/- 0.04, 0.47 +/- 0.03, 0.39 +/- 0.02, and 0.33 +/- 0.01, respectively. This morphological effect was inhibited at 24 and 48 h but not at 3 and 6 h by a 20 min pre-exposure to pulsed US (1.5 MHz, 30 mW/cm(2)). Shear stress significantly decreased Bone Morphogenetic Protein-4 (BMP-4) mRNA levels at 1, 2, 3, 6, and 24 h by 32.5 +/- 1.8%, 30.8 +/- 3.5%, 49.6 +/- 2.8%, 23.5 +/- 5.0%, 24.4 +/- 2.3%, respectively. A 20 min pulsed US exposure had no significant effect. However, a 20 min pre-exposure to pulsed US caused significant 39.6 +/- 3.0% and 25.6 +/- 2.7% decreases in BMP-4 levels in shear stress treated cells at 3 and 24 h, respectively. These results show for the first time that pulsed US alters the mechanotransductive effects of shear stress indicating a more comprehensive understanding of therapeutic US will be obtained when it is studied in conjunction with in vivo, regulatory biomechanical forces.

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Low-Intensity Pulsed Ultrasound Enhances Early Healing of Medial Collateral Ligament Injuries in Rats

Abstract: Low-intensity pulsed ultrasound exposure is effective for enhancing the early healing of medial collateral ligament injuries.

Cited by 55 publications

References 13 publications

Can ultrasound counteract bone loss? Effect of low-intensity ultrasound stimulation on a model of osteoclastic precursor

Can ultrasound counteract bone loss? Effect of low-intensity ultrasound stimulation on a model of osteoclastic precursor

Low-Intensity Pulsed Ultrasound Modulates Shear Stress Induced PGHS-2 Expression and PGE2 Synthesis in MLO-Y4 Osteocyte-Like Cells

Interdependence of Pulsed Ultrasound and Shear Stress Effects on Cell Morphology and Gene Expression

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