Low Genetic Variation in Subpopulations of an Endangered Clonal Plant<i>Iris sibirica</i>in Southern Poland

Kostrakiewicz, K.; Wróblewska, Ada

doi:10.5735/085.045.0303

Cited by 11 publications

(7 citation statements)

References 41 publications

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“…These studies have shown variable results. While Dittbrenner et al (2005) found a similar level of genetic variability as revealed for most glacial relict species, Kostrakiewicz and Wróblewska (2008) found very low level of genetic variability, similar to endemic species. The results of the latter study may be related to the extensive clonal growth in Iris sibirica.…”

Section: Introductionsupporting

confidence: 53%

“…In Europe such species occur in few isolated localities (Meusel and Jäger, 1992). To our knowledge, genetic variability of relict west range-peripheral populations was studied only in Angelica palustris (Dittbrenner et al, 2005), Iris aphylla (Wróblewska, 2008;Wróblewska et al, 2003), Iris sibirica (Kostrakiewicz and Wróblewska, 2008) and Stipa capillata (Hensen et al, 2009;Krzakowa and Michalak, 2007). These studies have shown variable results.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of a relict plant species, Ligularia sibirica (L.) Cass. (Asteraceae)

Šmídová

Münzbergová

Plačková

2011

Flora - Morphology, Distribution, Functional Ecology of Plants

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Section: Introductionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Genetic diversity of a relict plant species, Ligularia sibirica (L.) Cass. (Asteraceae)

Šmídová

Münzbergová

Plačková

2011

Flora - Morphology, Distribution, Functional Ecology of Plants

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“…It is a rhizomatous plant, up to 120 cm tall with long narrow leaves and monochasial cymose inflorescence with 1-7 blue-liliac actinomorphic flowers (Szöllősi et al, 2010(Szöllősi et al, , 2011. It is native to wet fields and mountain regions of the Northern Hemisphere and listed as an endangered and protected species in many countries such as Poland (Kostrakiewicz and Wróblewska, 2008) and Hungary (Szöllősi et al, 2011). I. sibirica plants, like Iris pallida and Iris germanica, accumulate in their roots essential oils, whose constituent terpenoid ketone-irone with strong violet-like scent is widely used in perfumery and cosmetics (Aslanyants and Marshavina, 1979;Gozu et al, 1993;Jéhan et al, 1994).…”

Section: Introductionmentioning

confidence: 98%

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

Stanišić

Raspor

Ninković

et al. 2015

South African Journal of Botany

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Efficient protocols, safe from somaclonal variation, were developed for regeneration of Iris sibirica plants via organogenesis and somatic embryogenesis from leaf-base explants cultivated on Murashige and Skoog media supplemented with thidiazuron (TDZ, 1.0 mg/l) or 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l). The morphogenic response and callus formation efficiency differed significantly between 2,4-D (80.9%) and TDZ (67%) morphogenesis induction treatments. TDZ induced only organogenic calli, while calli obtained with 2,4-D were composed of three types differing in color and consistency: white, friable -embryogenic calli (4.5%, 3.8 mg/explant), green, compact -organogenic calli (12.4%, 48.4 mg/explants) and yellow -nonregenerative calli (77.3%, 254.4 mg/explant). The cultivation of embryogenic calli on medium with 2,4-D and Kinetin resulted in further development of somatic embryos (54 embryos/g of calli) which germinated with a frequency of 62% after being transferred to a medium without plant growth regulators. Stable shoot cultures were established by transferring organogenic calli with shoot primordia to media with 0.1 mg/l α-naphthaleneacetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine (BA), while further cultivation on media of the same composition (TDZ or 2,4-D) resulted in the reduced growth and rhizogenesis, respectively. The TDZ induction treatment resulted in higher number of shoots per explant (7.9) than the 2,4-D treatment (4.3). After successful rooting and ex vitro acclimatization, plants were grown in the field and flowered to seed production. Flow cytometry, chromosome counting and random amplification of polymorphic DNA (RAPD) analysis indicated no evidence of genetic variation in plants regenerated via somatic embryogenesis or organogenesis. The results suggest that established protocols are safe for use in genetic transformation procedures or large-scale production of true-to-type I. sibirica plants.

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“…Secondary succession frequently leads to the fragmentation of communities and isolation of populations [5][6][7]. Small and isolated populations may suffer from a low level of genetic diversity and a high risk of individual mortality, frequently resulting in their extinction [8][9][10][11]. On the other hand, Czarnecka and Denisow [12] argued that genetic variability of small populations might be improved by long-distance moving pollinators.…”

Section: Introductionmentioning

confidence: 99%

The effect of the shape of gaps on microenvironmental conditions and seedling recruitment in Molinietum caeruleae meadows

Kostrakiewicz-Gierałt

2015

Acta Agrobot

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Cessation of the management of semi-natural habitats such as grasslands and meadows contributes to secondary succession and encroachment of native and alien tall-growing perennials, large tussock grasses, shrubs, and trees. Thus, the formation of gaps in the plant canopy and litter, enabling seedling recruitment, appears to be a very effective method for the restoration of several plant communities. The main objective of the research was to assess the effect of the shape of openings on microenvironmental conditions and seedling recruitment in Molinietum caeruleae patches in various habitat conditions. In all study patches, circular and linear openings, comparable in area, were randomly created through the removal of plant canopy and litter layer. The circular gaps presented greater light availability and lower soil humidity than linear openings, while soil temperature within differently shaped openings was similar. Regardless of differences in microenvironmental conditions, the total number of seedlings in differently shaped gaps did not vary considerably. Three plant categories were found: (i) those recruited mostly in circular openings, (ii) those recruited mostly in linear gaps, (iii) those colonizing circular and linear gaps similarly. The colonizers of circular gaps represented various synecological groups (ruderal, grasslands and meadows, young tree communities) and diverse life forms (therophytes, hemicryptophytes, chamaephytes, phanerophytes), while the colonizers of linear gaps were meadow and grassland hemicryptophytes. The formation of linear openings contributes to increases in the abundance of meadow taxa, while the creation of circular openings may have a negative effect, contributing to the promotion of the secondary succession process.

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Low Genetic Variation in Subpopulations of an Endangered Clonal PlantIris sibiricain Southern Poland

Cited by 11 publications

References 41 publications

Genetic diversity of a relict plant species, Ligularia sibirica (L.) Cass. (Asteraceae)

Genetic diversity of a relict plant species, Ligularia sibirica (L.) Cass. (Asteraceae)

Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture — RAPD and flow cytometer analyses

The effect of the shape of gaps on microenvironmental conditions and seedling recruitment in Molinietum caeruleae meadows

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