Low-Frequency Vibration Promotes Tumor Necrosis Factor-α Production to Increase Cartilage Degeneration in Knee Osteoarthritis

Abstract

Objective Low-frequency vibration accelerates cartilage degeneration in knee osteoarthritis (KOA) rat model. In this article, we investigated whether whole-body vibration (WBV) increases cartilage degeneration by regulating tumor necrosis factor-α (TNF-α) in KOA. Design Proteomics analysis was used to filter candidate protein from synovial fluid (SF) in KOA people after WBV. Enzyme-linked immunosorbent assay (ELISA) was used to estimate changes in TNF-α levels in SF. The C57 mice and TNF-α knock-out mice were sacrificed for the KOA model and WBV intervention. The cartilage was tested by ELISA, histology, terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL), immunohistochemistry, and reverse transcriptase polymerase chain reaction. Luciferase activity test in vitro study was conducted to confirm the relationship between TNF-α and the candidate protein. Results Differentially expressed proteins were enriched in the glycolytic process, glucose catabolic, and regulation of interleukin-8 (IL-8) secretion processes. Phosphoglycerate kinase, triosephosphate isomerase 1, T cell immunoglobulin- and mucin-domain-containing molecules 2, fumarylacetoacetate hydrolase (FAH), and TNF were the hub node. TNF-α expression increased in SF after WBV ( P < 0.05). The cartilage was more degenerated in the TNF-α−/− mice group compared to controls. A significant change was observed in collagen II and FAH ( P < 0.05). TNF-α expression improved in C57 mice ( P < 0.05). Apoptosis of chondrocytes was inhibited in TNF-α−/− mice by the TUNEL test. Luciferase activity significantly increased in TNF-α + FAH-Luc cells ( P < 0.05). Conclusion A novel mechanism underlying WBV-triggered cartilage degeneration was found in KOA that demonstrated the critical regulatory function of TNF-α and FAH during WBV.

Knee osteoarthritis: A review of animal models and intervention of traditional Chinese medicine

“…The medial tibial meniscus ligament was excised in 3‐month‐old male C57BL/6J skeletal mature mice to establish an unstable medial meniscus model. It has been found that low‐frequency vibration promotes the production of TNF‐α and thus increases cartilage degeneration in KOA 69 . Kaneko et al 70 .…”

“…It has been found that low‐frequency vibration promotes the production of TNF‐α and thus increases cartilage degeneration in KOA. 69 Kaneko et al. 70 used meniscectomy to construct a rat model of KOA and demonstrated that oral administration of N ‐acetylcysteine slowed the development of KOA in the rat model.…”

“…It has been found that low-frequency vibration promotes the production of TNFα and thus increases cartilage degeneration in KOA. 69 Kaneko et al 70 used meniscectomy to construct a rat model of KOA and demonstrated that oral administration of N-acetylcysteine slowed the development of KOA in the rat model. In this study, the model was constructed using a parapatellar approach to reach the intra-articular space of the knee through a median incision above the patella.…”