Low frequency stimulation induces polarization-based capturing of normal, cancerous and white blood cells: a new separation method for circulating tumor cell enrichment or phenotypic cell sorting

Jahangiri, Mojtaba; Ranjbar-Torkamani, Mina; Abadijoo, Hamed; Ghaderinia, Mohammadreza; Ghafari, Hadi; Mamdouh, Amir; Abdolahad, Mohammad

doi:10.1039/d0an01033b

Cited by 10 publications

(7 citation statements)

References 24 publications

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“…The 20 nm Cr layer was used in order to enhance the 100 nm Au layer adhesion to the glass substrate. Using standard photolithography ( Jahangiri et al, 2019 ; 2020 ), the eight electrodes were patterned on the surface of Au coated glass. The patterned electrodes were passivated using a 4 µm layer of PDMS by spin coating at 6000 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Healing Field: Using Alternating Electric Fields to Prevent Cytokine Storm by Suppressing Clonal Expansion of the Activated Lymphocytes in the Blood Sample of the COVID-19 Patients

Abadijoo

Khayamian

Faramarzpour

et al. 2022

Front. Bioeng. Biotechnol.

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In the case of the COVID-19 early diagnosis, numerous tech innovations have been introduced, and many are currently employed worldwide. But, all of the medical procedures for the treatment of this disease, up to now, are just limited to chemical drugs. All of the scientists believe that the major challenge toward the mortality of the COVID-19 patients is the out-of-control immune system activation and the subsequent cytokine production. During this process, the adaptive immune system is highly activated, and many of the lymphocytes start to clonally expand; hence many cytokines are also released. So, any attempt to harness this cytokine storm and calm down the immune outrage is appreciated. While the battleground for the immune hyperactivation is the lung ambient of the infected patients, the only medical treatment for suppressing the hypercytokinemia is based on the immunosuppressor drugs that systemically dampen the immunity with many unavoidable side effects. Here, we applied the alternating electric field to suppress the expansion of the highly activated lymphocytes, and by reducing the number of the renewed cells, the produced cytokines were also decreased. Applying this method to the blood of the COVID-19 patients in vitro showed ∼33% reduction in the average concentration of the three main cytokines after 4 days of stimulation. This method could carefully be utilized to locally suppress the hyperactivated immune cells in the lung of the COVID-19 patients without any need for systemic suppression of the immune system by the chemical drugs.

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Section: Methodsmentioning

confidence: 99%

Healing Field: Using Alternating Electric Fields to Prevent Cytokine Storm by Suppressing Clonal Expansion of the Activated Lymphocytes in the Blood Sample of the COVID-19 Patients

Abadijoo

Khayamian

Faramarzpour

et al. 2022

Front. Bioeng. Biotechnol.

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“…Due to the difference in dielectric properties, such as polarization constant between different kinds of cells, cells can be distinguished by applying an appropriate electric field. Jahangiri et al (2020) completed the separation of CTCs and blood cells from different kinds of breast cancer by applying a low-frequency alternating current (AC) electric field on the chip ( Figure 3A ). Gascoyne et al (2009) separated and screened CTCs in blood under the condition of an external AC electric field ( Figure 3B ), realized the enrichment of CTCs, and obtained more than 90% cell capture rate with a fast processing time and much higher separation efficiency than biological affinity method.…”

Section: Microfluidic Technologies For Ctcs Separationmentioning

confidence: 99%

“… Active enrichment methods based on physical screening (A) The CTCs detection chip based on AC electric field ( Jahangiri et al, 2020 ); (B) The DEP flow fractionation (depFFF) configuration in CTCs detection ( Gascoyne et al, 2009 ); (C) The acoustic CTC separation device ( Wu et al, 2018 ); (D) Size insensitive isolation of CTCs from PBMCs ( Karthick et al, 2018 ); (E) The microfluidic device on a glass slide with anti-EpCAM modification on the micropost in the device ( Sun et al, 2019 ); (F) The two-step acoustophoresis ( Undvall Anand et al, 2021 ). …”

Section: Microfluidic Technologies For Ctcs Separationmentioning

confidence: 99%

Application of Microfluidics in Detection of Circulating Tumor Cells

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Tumor metastasis is one of the main causes of cancer incidence and death worldwide. In the process of tumor metastasis, the isolation and analysis of circulating tumor cells (CTCs) plays a crucial role in the early diagnosis and prognosis of cancer patients. Due to the rarity and inherent heterogeneity of CTCs, there is an urgent need for reliable CTCs separation and detection methods in order to obtain valuable information on tumor metastasis and progression from CTCs. Microfluidic technology is increasingly used in various studies of CTCs separation, identification and characterization because of its unique advantages, such as low cost, simple operation, less reagent consumption, miniaturization of the system, rapid detection and accurate control. This paper reviews the research progress of microfluidic technology in CTCs separation and detection in recent years, as well as the potential clinical application of CTCs, looks forward to the application prospect of microfluidic technology in the treatment of tumor metastasis, and briefly discusses the development prospect of microfluidic biosensor.

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Section: Ctc Biology and Detection Technologymentioning

confidence: 99%

“…Manipulating the polarity regulators or generic depolarization may affect transmigration and metastasis of CTCs in vitro and in vivo, and thereby contributing to a part of targeted therapy. 53 In fact, only a small subset of CTCs is capable of successful metastasis, therefore these cells are termed circulating cancer stem cells (cCTCs 54 ). cCTCs bear metastasis-initiating capabilities based on their stemness properties and invasiveness.…”

Section: Ctc Biology and Detection Technologymentioning

confidence: 99%

Cancer metastasis chemoprevention prevents circulating tumour cells from germination

Xie

Lian

et al. 2022

Sig Transduct Target Ther

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The war against cancer traces back to the signature event half-a-century ago when the US National Cancer Act was signed into law. The cancer crusade costs trillions with disappointing returns, teasing the possibility of a new breakthrough. Cure for cancer post-metastases still seems tantalisingly out of reach. Once metastasized, cancer-related death is extremely difficult, if not impossible, to be reversed. Here we present cancer pre-metastasis chemoprevention strategy that can prevent circulating tumour cells (CTCs) from initiating metastases safely and effectively, and is disparate from the traditional cancer chemotherapy and cancer chemoprevention. Deep learning of the biology of CTCs and their disseminating organotropism, complexity of their adhesion to endothelial niche reveals that if the adhesion of CTCs to their metastasis niche (the first and the most important part in cancer metastatic cascade) can be pharmaceutically interrupted, the lethal metastatic cascade could be prevented from getting initiated. We analyse the key inflammatory and adhesive factors contributing to CTC adhesion/germination, provide pharmacological fundamentals for abortifacients to intervene CTC adhesion to the distant metastasis sites. The adhesion/inhibition ratio (AIR) is defined for selecting the best cancer metastasis chemopreventive candidates. The successful development of such new therapeutic modalities for cancer metastasis chemoprevention has great potential to revolutionise the current ineffective post-metastasis treatments.

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Low frequency stimulation induces polarization-based capturing of normal, cancerous and white blood cells: a new separation method for circulating tumor cell enrichment or phenotypic cell sorting

Abstract: Separation of cancerous from normal cells is of broad importance in a large number of cancer diagnosis and treatment methods. One of the most important factors to designate and specify...

Cited by 10 publications

References 24 publications

Healing Field: Using Alternating Electric Fields to Prevent Cytokine Storm by Suppressing Clonal Expansion of the Activated Lymphocytes in the Blood Sample of the COVID-19 Patients

Healing Field: Using Alternating Electric Fields to Prevent Cytokine Storm by Suppressing Clonal Expansion of the Activated Lymphocytes in the Blood Sample of the COVID-19 Patients

Application of Microfluidics in Detection of Circulating Tumor Cells

Cancer metastasis chemoprevention prevents circulating tumour cells from germination

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