Low frequency of LRV1 in Leishmania braziliensis strains isolated from typical and atypical lesions in the State of Minas Gerais, Brazil

Macedo, Diego H.; Menezes-Neto, Armando de; Rugani, Jeronimo Marteleto Nunes; Rocha, Ana C.; Silva, Soraia O.; Melo, Maria Norma; Lye, Lon-Fye; Beverley, Stephen M.; Gontijo, Célia Maria Ferreira; Soares, Rodrigo Pedro

doi:10.1016/j.molbiopara.2016.08.005

Cited by 25 publications

(28 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…After reading the full text of the articles, 17 papers were included in our systematic review and meta‐analysis (Tarr et al, ; Widmer et al, ; Saiz et al, ; Guilbride et al, ; Ogg, Carrion, J.r. R., Botelho, A.C., Mayrink, W., Correa‐Oliveira, R., Patterson, & J.L., ; Pereira, ; Valencia et al, ; Zangger et al, ; Adaui et al, ; Bourreau et al, ; Cantanhede et al, ; Ito et al, ; Ginouvès et al, ; Hajjaran et al, ; Macedo et al, ; Sukla, Roy, Sundar, & Biswas, ; Kariyawasam et al, ).…”

Section: Resultsmentioning

confidence: 99%

Global status of synchronizing Leishmania RNA virus in Leishmania parasites: A systematic review with meta‐analysis

Saberi

Fakhar

Mohebali

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

Leishmaniasis is one of the most neglected tropical diseases caused by protozoan parasites belonging to the genus Leishmania. There is much evidence regarding prevalence of Leishmania RNAvirus (LRV) causing Old World leishmaniasis (OWL) and New World leishmaniasis (NWL); however, a combined evidence‐based knowledge on this topic is not still available. The purpose of this systematic review and meta‐analysis was to address the global status of synchronizing LRV in Leishmania in the available literature. The data were systematically collected from the English electronic databases up to May 2018. Then, the studies were screened based on the inclusion and exclusion criteria. The random‐effect model was used by forest plot with 95% confidence interval (CI). Overall, 877 samples from 17 articles were included in this study. Given species of Leishmania, the highest prevalence of LRV belonged to Leishmania (L.) Viannia (V.) guyanensis and L. V. braziliensis. Additionally, the virus was detected also in L. V. amazonensis, L. V. panamanensis, L. V. lainsoni, L. aethiopica, L. major and L. infantum. By random‐effect model, the global prevalence of LRV was estimated to be 26.2% (95% CI: 14.4% ‐ 40.1%). The high prevalence of LRV among causative agents of NWLisolated from the metastatic clinical forms suggests potential association of LRV with metastatic clinical forms in New World endemic regions. A comprehensive investigation on experimental and clinical aspects of LRV is needed to fully appraise the role of these viruses in pathogenicity of Leishmania parasites and their drug resistance.

show abstract

Section: Resultsmentioning

confidence: 99%

Global status of synchronizing Leishmania RNA virus in Leishmania parasites: A systematic review with meta‐analysis

Saberi

Fakhar

Mohebali

et al. 2019

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Indeed, knowledge of the genomic sequence of many LRV1 strains was essential for oligonucleotide design ensuring adequate detection of the virus (100% detection rate for LRV1s-LRV2as and 5LRVs1-LRV11as/LRV14as). It is noteworthy that the primers and probes that have previously been described, whether used for quantification or identification of LRV1 in clinical samples, possessed mismatches relative to our sequence data set [ 22 , 26 , 29 , 32 , 48 – 50 ]. This could have led to underestimation of the viral load or under-detection of divergent unknown strains.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana

Tirera¹,

Ginouvès²,

Donato³

et al. 2017

PLoS Negl Trop Dis

View full text Add to dashboard Cite

IntroductionLeishmania RNA virus type 1 (LRV1) is an endosymbiont of some Leishmania (Vianna) species in South America. Presence of LRV1 in parasites exacerbates disease severity in animal models and humans, related to a disproportioned innate immune response, and is correlated with drug treatment failures in humans. Although the virus was identified decades ago, its genomic diversity has been overlooked until now.Methodology/Principles findingsWe subjected LRV1 strains from 19 L. (V.) guyanensis and one L. (V.) braziliensis isolates obtained from cutaneous leishmaniasis samples identified throughout French Guiana with next-generation sequencing and de novo sequence assembly. We generated and analyzed 24 unique LRV1 sequences over their full-length coding regions. Multiple alignment of these new sequences revealed variability (0.5%–23.5%) across the entire sequence except for highly conserved motifs within the 5’ untranslated region. Phylogenetic analyses showed that viral genomes of L. (V.) guyanensis grouped into five distinct clusters. They further showed a species-dependent clustering between viral genomes of L. (V.) guyanensis and L. (V.) braziliensis, confirming a long-term co-evolutionary history. Noteworthy, we identified cases of multiple LRV1 infections in three of the 20 Leishmania isolates.Conclusions/SignificanceHere, we present the first-ever estimate of LRV1 genomic diversity that exists in Leishmania (V.) guyanensis parasites. Genetic characterization and phylogenetic analyses of these viruses has shed light on their evolutionary relationships. To our knowledge, this study is also the first to report cases of multiple LRV1 infections in some parasites. Finally, this work has made it possible to develop molecular tools for adequate identification and genotyping of LRV1 strains for diagnostic purposes. Given the suspected worsening role of LRV1 infection in the pathogenesis of human leishmaniasis, these data have a major impact from a clinical viewpoint and for the management of Leishmania-infected patients.

show abstract

“…At the epidemiologic level, LRV1/2 has been found at frequencies ranging from 0 to 87% in clinical samples from patients with leishmaniasis (15, 17, 19–33); the strength of the association between viral presence and complication development varies according to the study (24, 28) and among different South-American Regions (30). The wide range in LRV1 frequency of detection in clinical samples may reflect diverse experimental approaches that do not account for differences in clinical specimens.…”

Section: Introductionmentioning

confidence: 99%

Toward an objective diagnostic for detection of Leishmania RNA virus (LRV) in clinical samples from cutaneous leishmaniasis patients

Parra-Muñoz

Aponte

Ovalle-Bracho

et al. 2018

Preprint

View full text Add to dashboard Cite

18Leishmania RNA virus (LRV) is a double strand RNA virus belonging to the was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/338095 doi: bioRxiv preprint first posted online Jun. 4, 2018; 2 and treatment failure of cutaneous leishmaniasis (TFCL). However, the reported

show abstract

Low frequency of LRV1 in Leishmania braziliensis strains isolated from typical and atypical lesions in the State of Minas Gerais, Brazil

Cited by 25 publications

References 27 publications

Global status of synchronizing Leishmania RNA virus in Leishmania parasites: A systematic review with meta‐analysis

Global status of synchronizing Leishmania RNA virus in Leishmania parasites: A systematic review with meta‐analysis

Unraveling the genetic diversity and phylogeny of Leishmania RNA virus 1 strains of infected Leishmania isolates circulating in French Guiana

Toward an objective diagnostic for detection of Leishmania RNA virus (LRV) in clinical samples from cutaneous leishmaniasis patients

Contact Info

Product

Resources

About