Low-frequency dynamics of DNA in Brillouin light scattering spectra

Lushnikov, S. G.; Дмитриев, А. В.; Fedoseev, A. I.; Захаров, Г. А.; Zhuravlev, Aleksandr V.; Medvedeva, A. V.; Schegolev, B. F.; Савватеева-Попова, Е. В.

doi:10.1134/s0021364013240168

Cited by 6 publications

(6 citation statements)

References 14 publications

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“…It was found that a temperature increase from room temperature leads to significant changes in the biopolymer structure during denaturing, the formation of various forms of aggregates, and further transformations into gel‐like structures . The research into the thermal denaturing of DNA and proteins has shown that anomalies in the behavior of Rayleigh‐Brillouin light‐scattering spectra reflect the sequence of high‐temperature phase transformations of biopolymers . For example, the transition to the “molten globule” state in lysozyme, which preceded thermal denaturing was accompanied by anomalies in the behavior of Brillouin doublets and changes in rigidity .…”

Section: Introductionmentioning

confidence: 99%

“…[2,12,13] The research into the thermal denaturing of DNA and proteins has shown that anomalies in the behavior of Rayleigh-Brillouin light-scattering spectra reflect the sequence of high-temperature phase transformations of biopolymers. [14][15][16][17] For example, the transition to the "molten globule" state in lysozyme, which preceded thermal denaturing was accompanied by anomalies in the behavior of Brillouin doublets and changes in rigidity. [15] A critical opalescence at which the intensities of the Brillouin and Rayleigh components of the spectra change significantly appeared in the vicinity of lysozyme denaturing.…”

Section: Introductionmentioning

confidence: 99%

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Phase transformations of bovine serum albumin: Evidences from Rayleigh‐Brillouin light scattering

Дмитриев

Vashchenkov

Fedoseev

et al. 2019

J Raman Spectroscopy

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Rayleigh‐Brillouin light‐scattering studies of a high‐concentration solution of bovine serum albumin (BSA, pH = 7.45, C = 100 mg/ml) in a sodium phosphate buffer in the temperature range 300–370 K are presented. It is shown that the temperature dependences of relative changes in the Brillouin shift and half width at half maximum and intensity of the Brillouin doublet and intensity of Rayleigh components exhibit anomalies in the vicinity of 335, 345, and 363 K. Analysis of the literature data allowed us to construct a phase diagram showing a sequence of BSA phase transformations in the temperature range from 300 to 375 K and to correlate the anomalies in the scattering spectra to the regions of phase transformations. The anomalies in the behavior of the light‐scattering spectra in the vicinity of Tm = 335 K are attributable to the melting temperature and the formation of BSA self‐assembled aggregates. The formation of a phase with oriented β‐sheet rich mature fibrils at Tm < T < 347 K is confirmed by elastic light scattering experiments. This phase passes into a gel‐like phase in which the aggregates are rearranged and a polymer network, which is finally converted to a gel in the vicinity of 363 K is formed. Thus, the Rayleigh‐Brillouin scattering experiments demonstrated the evolution of the structure, which manifested itself in the anomalous temperature behavior of the intensities of the Rayleigh and Brillouin components in the light‐scattering spectra. The anomalous low‐frequency dynamics of the BSA solution at denaturing and transition into a gel phase was clearly observed in the anomalous Brillouin shift behavior in the vicinity of 335 and 363 К. The sequence of phase transformations in BSA at heating from 300 to 375 K in the phase diagram correlates well with the anomalies observed in the evolution of Rayleigh‐Brillouin light scattering.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phase transformations of bovine serum albumin: Evidences from Rayleigh‐Brillouin light scattering

Дмитриев

Vashchenkov

Fedoseev

et al. 2019

J Raman Spectroscopy

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“…Local changes in DNA conformation with deviations from the classical B structure accompany many cellular processes such as transcription, replica tion, repair, etc. [51,52]. Conformational rearrange ments of DNA include hairpins, bubbles, triplexes, qua druplexes, and cruciform structures.…”

Section: Neuroepigeneticsmentioning

confidence: 99%

Neurogenetics and neuroepigenetics

2015

Self Cite

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Behavioral genetics or "Neurogenetics," is based on the evolutionary ideas of T. Dobzhansky on brain development and behavior. It continues with the "experimental genetics of higher nervous activity" of I. Pavlov and uses a comparative approach in the study of heredity and variation in behavioral manifestations, from Protozoa to humans. The study of the classical Pavlovian conditioned reflex in mutant Drosophila helped to identify the main types of memory and their evolutionary conservatism. Long term memory defects are caused by mutations of the same genes as in mental retardation in humans, when signaling cascades inter secting with the cAMP dependent pathway are damaged. The cascade of actin remodeling is also among these. The key enzyme, LIM kinase 1, controls cognitive manifestations of the "genomic disease" Williams deletion syndrome. Its study resulted in the recognition of neuroepigenetics as an interface between the genome and environmental influences. Epigenetic factors of "variability"-DNA methylation, histone acetylation, and miRNA regulation-do not change the structure of the gene but its manifestations. Certain miRNAs have already been considered to be both biomarkers for neurodegenerative diseases and factors of the transgenerational transmission of the behaviorial properties of ancestors who experienced stress from adverse environmental influences.

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“…which follows from Eqs. (33) and (34). It should also be noted that for spatially homogeneous initial conditions −h 2 (t) ≡ 0 (all integrals in this case must be considered in the sense of their values per unit length along the x axis), all inequalities of this section become equalities; among other things, inequality (31) becomes an ordinary differential equation for a Newtonian particle.…”

Section: Conditions For Explosive Development Of the Quantum Kelvmentioning

confidence: 99%

“…Analysis of the formation of singularity in Eq. (23) can be performed based on the method proposed in [33] (see [34][35][36] for the further development of this method). The method is based on analogy of Eq.…”

Section: Conditions For Explosive Development Of the Quantum Kelvmentioning

confidence: 99%

Explosive Development of the Kelvin–Helmholtz Quantum Instability on the He-II Free Surface

Зубарев

Lushnikov

2019

J. Exp. Theor. Phys.

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We analyze nonlinear dynamics of the Kelvin-Helmholtz quantum instability of the He-II free surface, which evolves during counterpropagation of the normal and superfluid components of liquid helium. It is shown that in the vicinity of the linear stability threshold, the evolution of the boundary is described by the |φ| 4 Klein-Gordon equation for the complex amplitude of the excited wave with cubic nonlinearity. It is important that for any ratio of the densities of the helium component, the nonlinearity plays a destabilizing role, accelerating the linear instability evolution of the boundary. The conditions for explosive growth of perturbations of the free surface are formulated using the integral inequality approach. Analogy between the Kelvin-Helmholtz quantum instability and electrohydrodynamic instability of the free surface of liquid helium charged by electrons is considered.I.

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Low-frequency dynamics of DNA in Brillouin light scattering spectra

Cited by 6 publications

References 14 publications

Phase transformations of bovine serum albumin: Evidences from Rayleigh‐Brillouin light scattering

Phase transformations of bovine serum albumin: Evidences from Rayleigh‐Brillouin light scattering

Neurogenetics and neuroepigenetics

Explosive Development of the Kelvin–Helmholtz Quantum Instability on the He-II Free Surface

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