Low-Frequency Components in Rat Pial Arteriolar Rhythmic Diameter Changes

Lapi, Dominga; Mastantuono, Teresa; Maro, Martina Di; Varanini, M.; Colantuoni, Antonio

doi:10.1159/000478984

Cited by 6 publications

(8 citation statements)

References 29 publications

(35 reference statements)

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“…The diameter measures used for the analyses were obtained on 30 min tracings (acquisition frequency: 4 Hz) to obtain the resolution of the six components in the following ranges: 2.5–4.5 Hz (termed VHF, i.e., very high frequency), 0.2–2.0 Hz (HF, high frequency), 0.06–0.2 Hz (LF, low frequency),0.02–0.06 Hz (ILF, infra-low frequency), 0.0095–0.021 Hz (VLF, very low frequency), and 0.001–0.0095 Hz (ULF, ultra-low frequency; Lapi et al, 2010 ).…”

Section: Methodsmentioning

confidence: 99%

Repeated Mandibular Extension in Rat: A Procedure to Modulate the Cerebral Arteriolar Tone

et al. 2017

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Previous data have shown both in the rat and in the human that a single mandibular extension lasting 10 min induces a significant important and prolonged reduction in blood pressure and heart rate, affecting also rat pial microcirculation by the release of endothelial factors. In the present work, we assessed whether repeated mandibular extension could further prolong these effects. We performed two mandibular extensions, the second mandibular extension being applied 10 min after the first one. The second mandibular extension produced a reduction in blood pressure and heart rate for at least 240 min. As in the case of a single mandibular extension, pial arterioles dilated persisting up to 140 min after the second extension. Spectral analysis on 30 min recordings under baseline conditions and after repetitive mandibular extensions showed that the pial arterioles dilation was associated with rhythmic diameter changes sustained by an increase in the frequency components related to endothelial, neurogenic, and myogenic activity while a single mandibular extension caused, conversely, an increase only in the endothelial activity. In conclusion, repetitive mandibular extension prolonged the effects of a single mandibular extension on blood pressure, heart rate and vasodilation and induced a modulation of different frequency components responsible of the pial arteriolar tone, in particular increasing the endothelial activity.

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Section: Methodsmentioning

confidence: 99%

Repeated Mandibular Extension in Rat: A Procedure to Modulate the Cerebral Arteriolar Tone

et al. 2017

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“…Moreover, the components into ranges 0.021–0.052 Hz and 0.052–0.145 Hz were correlated to the neurogenic and the intrinsic myogenic activity of vascular smooth muscle cells, respectively. Finally, the ranges 0.145–0.6 Hz and 0.6–2.0 Hz have been associated to the respiration and the heart rates, respectively (Kvandal et al, 2003 , 2006 ; Lapi et al, 2010 ). Our data indicate that oscillations in rat pial microcirculation presented quite the same ranges previously reported with the exception of the frequency component related to the heart rate.…”

Section: Discussionmentioning

confidence: 99%

“…In rat brain, it has been reported that oscillatory patterns characterize pial blood flow (Hudetz et al, 1992 ; Ursino et al, 1998 ; Mastantuono et al, 2016a ). However, a large number of studies have shown that these oscillations in blood flow are due to the several physiological factors, regulating microvascular blood flow redistribution (Kvandal et al, 2003 , 2006 ; Lapi et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

“…Four low frequency components in the ranges 0.001–0.0095 Hz, 0.0095–0.02 Hz, 0.02–0.06 Hz and 0.06–0.2 Hz were related to NO-independent and NO-dependent endothelial activity, to the neurogenic and to the intrinsic myogenic activity of vascular smooth muscle cells, respectively. Two high frequency components into ranges 0.2–2.0 Hz and 2.0–4.5 Hz have been associated to the respiration and the heart rates, respectively (Lapi et al, 2017 ). Therefore, the aim of the present study was to in vivo investigate the rat pial microcirculation by LSI; in particular, we tried to evaluate the frequency components in blood flow oscillations during brain hypoperfusion and reperfusion.…”

Section: Introductionmentioning

confidence: 99%

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Laser Speckle Imaging of Rat Pial Microvasculature during Hypoperfusion-Reperfusion Damage

Mastantuono

Starita

Battiloro

et al. 2017

Front. Cell. Neurosci.

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The present study was aimed to in vivo assess the blood flow oscillatory patterns in rat pial microvessels during 30 min bilateral common carotid artery occlusion (BCCAO) and 60 min reperfusion by laser speckle imaging (LSI). Pial microcirculation was visualized by fluorescence microscopy. The blood flow oscillations of single microvessels were recorded by LSI; spectral analysis was performed by Wavelet transform. Under baseline conditions, arterioles and venules were characterized by blood flow oscillations in the frequency ranges 0.005–0.0095 Hz, 0.0095–0.021 Hz, 0.021–0.052 Hz, 0.052–0.150 Hz and 0.150–0.500 Hz. Arterioles showed oscillations with the highest spectral density when compared with venules. Moreover, the frequency components in the ranges 0.052–0.150 Hz and 0.150–0.500 were predominant in the arteriolar total power spectrum; while, the frequency component in the range 0.150–0.500 Hz showed the highest spectral density in venules. After 30 min BCCAO, the arteriolar spectral density decreased compared to baseline; moreover, the arteriolar frequency component in the range 0.052–0.150 Hz significantly decreased in percent spectral density, while the frequency component in the range 0.150–0.500 Hz significantly increased in percent spectral density. However, an increase in arteriolar spectral density was detected at 60 min reperfusion compared to BCCAO values; consequently, an increase in percent spectral density of the frequency component in the range 0.052–0.150 Hz was observed, while the percent spectral density of the frequency component in the range 0.150–0.500 Hz significantly decreased. The remaining frequency components did not significantly change during hypoperfusion and reperfusion. The changes in blood flow during hypoperfusion/reperfusion caused tissue damage in the cortex and striatum of all animals. In conclusion, our data demonstrate that the frequency component in the range 0.052–0.150 Hz, related to myogenic activity, was significantly impaired by hypoperfusion and reperfusion, affecting cerebral blood flow distribution and causing tissue damage.

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“…The power density spectral distribution was obtained by time averaging the time-frequency power density representation. This technique permits to evaluate non-stationary data, such as those represented by rhythmic variations in vessel diameters” (Rossi et al, 2005; Lapi et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

Arterial Network Geometric Characteristics and Regulation of Capillary Blood Flow in Hamster Skeletal Muscle Microcirculation

et al. 2019

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This study was aimed to characterize the geometric arrangement of hamster skeletal muscle arteriolar networks and to assess the in vivo rhythmic diameter changes of arterioles to clarify regulatory mechanisms of the capillary perfusion. The experimental study was carried out in male Syrian hamsters implanted with a plastic chamber in the dorsum skin under pentobarbital anesthesia. The skeletal muscle microvessels were visualized by fluorescence microscopy. The vessel diameters, lengths and the rhythmic diameter changes of arterioles were analyzed with computer-assisted techniques. The arterioles were classified according to a centripetal ordering scheme. In hamster skeletal muscle microvasculature the terminal branchings, differentiated in long and short terminal arteriolar trees (TATs), originated from anastomotic vessels, defined “arcading” arterioles. The long TATs presented different frequencies along the branching vessels; order 4 arterioles had frequencies lower than those observed in the order 3, 2, and 1 vessels. The short TAT order 3 arterioles, directly originating from “arcading” parent vessels, showed a frequency dominating all daughter arterioles. The amplitude of diameter variations in larger vessels was in the range 30–40% of mean diameter, while it was 80–100% in order 3, 2, and 1 vessels. Therefore, the complete constriction of arterioles, caused an intermittent capillary blood perfusion. L-arginine or papaverine infusion caused dilation of arterioles and transient disappearing of vasomotion waves and induced perfusion of all capillaries spreading from short and long TAT arrangements. Therefore, the capillary blood flow was modulated by changes in diameter of terminal arterioles penetrating within the skeletal muscle fibers, facilitating redistribution of blood flow according to the metabolic demands of tissues.

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Low-Frequency Components in Rat Pial Arteriolar Rhythmic Diameter Changes

Cited by 6 publications

References 29 publications

Repeated Mandibular Extension in Rat: A Procedure to Modulate the Cerebral Arteriolar Tone

Repeated Mandibular Extension in Rat: A Procedure to Modulate the Cerebral Arteriolar Tone

Laser Speckle Imaging of Rat Pial Microvasculature during Hypoperfusion-Reperfusion Damage

Arterial Network Geometric Characteristics and Regulation of Capillary Blood Flow in Hamster Skeletal Muscle Microcirculation

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