Low frequency and low intensity pulsed electromagnetic field exerts its antiinflammatory effect through restoration of plasma membrane calcium ATPase activity

Selvam, Ramasamy; Ganesan, Kalaivani; Raju, K. Venkateswara; Gangadharan, Akkalayi Chandrapuram; Manohar, B Murali; Puvanakrishnan, Rengarajulu

doi:10.1016/j.lfs.2007.03.019

Cited by 67 publications

(70 citation statements)

References 30 publications

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“…However, several theories exist to explain these effects, for example the Larmor precession [13,14], the hypothesis of Gartzke and Lange [15] or radical pair mechanism [16-18] (for review see [19]). The application of the PEMF induces changes in cellular processes, among others, differentiation [20], apoptosis [21], DNA synthesis [22], protein expression [23], protein phosphorylation [24], anti-inflammatory effects [25] and hormone production [26]. …”

Section: Introductionmentioning

confidence: 99%

Low-frequency pulsed electromagnetic fields significantly improve time of closure and proliferation of human tendon fibroblasts

et al. 2014

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BackgroundThe promotion of the healing process following musculoskeletal injuries comprises growth factor signalling, migration, proliferation and apoptosis of cells. If these processes could be modulated, the healing of tendon tissue may be markedly enhanced. Here, we report the use of the Somagen™ device, which is certified for medical use according to European laws. It generates low-frequency pulsed electromagnetic fields that trigger effects of a nature that are yet to be determined.MethodsA 1.5-cm wide, linear scrape was introduced into patellar tendon fibroblast cultures (N = 5 donors). Treatment was carried out every second day. The regimen was applied three times in total with 30 minutes comprising pulsed electromagnetic field packages with two fundamental frequencies (10 minutes of 33 Hz, 20 minutes of 7.8 Hz). Control cells remained untreated. All samples were analyzed for gap closure time, proliferation and apoptosis one week after induction of the scrape wound.ResultsThe mean time for bridging the gap in the nontreated cells was 5.05 ± 0.33 days, and in treated cells, it took 3.35 ± 0.38 days (P <0.001). For cell cultures with scrape wounds, a mean value for BrdU incorporation of OD = 0.70 ± 0.16 was found. Whereas low-frequency pulsed electromagnetic fields treated samples showed OD = 1.58 ± 0.24 (P <0.001). However, the percentage of apoptotic cells did not differ between the two groups.ConclusionsOur data demonstrate that low-frequency pulsed electromagnetic fields emitted by the Somagen™ device influences the in vitro wound healing of patellar tendon fibroblasts and, therefore, possibly increases wound healing potential.

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Section: Introductionmentioning

confidence: 99%

Low-frequency pulsed electromagnetic fields significantly improve time of closure and proliferation of human tendon fibroblasts

et al. 2014

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“…An imbalance in the up-or downregulation of these signals can leave inflammation unchecked, resulting in cell and tissue damage. MF has been shown to be clinically beneficial in the repair of many types of cells and tissues through membrane and cytoskeletal organization in Cytokine-mediated effects of magnetic fields 67 receptor and kinase activity (Selvam et al, 2007;Hambleton et al, 1996;Rohde et al, 2009). Because the cell membrane has been proposed as the primary site of transduction for MF effects, relevant mechanisms may include changes in cellmembrane binding and transport processes, displacement or deformation of polarized molecules, and modifications in the conformation of biological water (Eichwald and Kaiser, 1995).…”

Section: Resultsmentioning

confidence: 99%

“…Immunological studies show that MF, even low-intensity MF, can interact with cells and tissues (Blank and Goodman, 1997;Markov et al, 2006). The antiinflammatory and wound healing applications of MF are well known (Selvam et al, 2007). For example, the effect of MF on the immune system in phagocytic cells alone has attracted attention because of the role that extremely low-frequency electromagnetic field (ELF-EMF) plays in decreasing the growth rate of bacteria (Akan et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of time-varied magnetic field on inflammatory response in macrophage cell line RAW 264.7

Ross¹,

Harrison²

2012

Electromagnetic Biology and Medicine

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The aim of this feasibility study was to determine the effectiveness of a commercially manufactured magnetic field (MF) device as an adjunct to pharmaceuticals during acute phase inflammatory response. The goal was to determine if inflammatory response interleukins IL-1, IL-6, IL-10, and tumor necrosis factor-alpha (TNF-α) would be affected by a 30 Hz time-varying magnetic field (MF). RAW 264.7 macrophage-like cells were induced with Gram-negative bacteria lipopolysaccharide (LPS) to initiate an acute inflammatory reaction. Following lipopolysaccharide (LPS) treatment, both inflamed and control cells were exposed to MF for 1 h. After MF exposure, cytokines of interest were measured and compared with controls. Outcomes revealed that LPS challenged cells continuously exposed to a 30 Hz time-varying magnetic field for 1 h demonstrated significant changes compared with controls. From cytokine test it was determined that MF exposure significantly decreased levels of IL-6 and IL-10 compared to unexposed counterparts. TNF-α production was significantly affected when MF was applied to cells only, but not to inflamed cells. Results suggest that the biological effect of 1 h exposure to a 30 Hz time-varied magnetic field may act to down regulate specific cytokines in an inflamed environment.

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“…Human lymphoide cells exposed to EMP (in 50 Hz, 2mT, 72h) modify the cytoskeletal organization, with increase of activity of protein kinase, without the cellular proliferation (Santono, Lisi, Pozzi et al, 1997). Selvam and associates showed that rats with induced arthritis exposed to the fi eld of 5Hz, 4μT, 90 minutes during 52 days there comes to the anti-infl ammatory effect through a change of activity of lymphocyte calcium ATP (Selvam et al, 2007). Reported was the decrease of level of infl ammatory mediator ta 1.95 mT PEMP-e ima pozitivno dejstvo na hondrocite, proizvodnju ekstracelularnog matriksa, njihovu diferencijaciju i citoskelet.…”

Section: Animal Studies On Magnetotherapy Effectmentioning

confidence: 99%

Contemporary Principles of Magnetotherapy Application in Physical Medicine and Rehabilitation // Savremeni principi primjene magnetoterapije u fizikalnoj medicini i rehabilitaciji

Popović¹

2018

SIZ

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Sažetak: Magnetoterapija je jedna od najstarijih metoda liječenja i kroz istoriju medicine prolazi put od alternativne do zvanične metode. U XXI vijeku veliki broj naučnih istraživanja proširuje indikaciona područja zasnovana na magnetoterapiji. Cilj rada je da se prikažu: istorijski razvoj, vrste magnetoterapije, njihovi biološke efekti, klinička primjena i mehanizmi djelovanja. Korištena je dostupna svjetska literatura iz oblasti bazičnih i kliničkih istraživanja o magnetoterapiji. Bazične studije ukazuju da leukociti, trombociti, osteoblasti, hondrociti, fi brinogen, fi brin, citokini, faktori rasta, kolagen, elastin i slobodni radikali pokazuju alteraciju u svom djelovanju kad su izloženi magnetnom polju. Magnetna polja utiču na proliferaciju ćelija, epitelizaciju, fagocitozu, vazodilataciju što svakako poboljšava fi ziološku sredinu koja doprinosi regeneraciji i izlječenju. Terapijski efekti zavise od svih karakteristika elektromagnetnog polja i od stanja pacijenta. Najširu primjenu pulsno elektromagnetno polje (PEMP) ima u stimulaciji osteogeneze (loše srasli prelomi, pseudoartroza, zarastanje spinalnih fuzija), osteoartritisa, osteoporoze i kod bolnih stanja. Transkranijalna magnetna stimulacija ima sve veću primjenu u neurorehabilitaciji. Precizni mehanizmi djelovanja elektromagnetne terapije još uvijek nisu poznati, što je svakako jedan od razloga različitih pristupa i nedovoljne i na dokazima utemeljene kliničke primjene ovog fi zikalnog modaliteta. Precizna dozimetrija, dobro defi nisani laboratorijski uslovi, dizajnirane kliničke studije, defi nisani protokoli liječenja doprinose jasnijom kliničkoj primjeni, kao i aktuelnosti magnetoterapije i u budućnosti. Ključne riječi: magnetoterapija, klinička primjena, pulsno elektromagnetno polje, transkranijalna magnetna stimulacija ISTORIJATPodaci o biološkim efektima magneta datiraju još iz vremena otkrića magnetizma u staroj Grčkoj. Magnetoterapija je fi zikalna terapija koja se najduže primijenjuje Correspondence: Tamara Popović Public Institution Medical College Prijedor tamaralukac@yahoo.comAbstract: Magnetotherapy is one of the oldest methods of treatment and throughout the history of medicine it passed the path from alternative to offi cial method. In the 21 st century a large number of scientifi c researches broadened the indication areas based on magnetotherapy. The objective of the paper is to demonstrate: historical development, types of magnetotherapy, its biological effects, clinical application and mechanisms of effects. Available world reference from the fi elds of basic and clinical researches on magnetotherapy was used. Basic studies indicate that leucocytes, thrombocytes, osteoblasts, chondrocytes, fi brinogen, fi brin, cytokine, the factors of growth, collagen, elastin and free radicals show the alteration in its effects when exposed to magnetic fi eld. Magnetic fi elds impact the proliferation of cells, epithelization, phagocytosis, vasodilation which certainly improves the physiological surrounding that contributes to the regeneration a...

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Low frequency and low intensity pulsed electromagnetic field exerts its antiinflammatory effect through restoration of plasma membrane calcium ATPase activity

Cited by 67 publications

References 30 publications

Low-frequency pulsed electromagnetic fields significantly improve time of closure and proliferation of human tendon fibroblasts

Low-frequency pulsed electromagnetic fields significantly improve time of closure and proliferation of human tendon fibroblasts

Effect of time-varied magnetic field on inflammatory response in macrophage cell line RAW 264.7

Contemporary Principles of Magnetotherapy Application in Physical Medicine and Rehabilitation // Savremeni principi primjene magnetoterapije u fizikalnoj medicini i rehabilitaciji

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