Low frequency and intensity ultrasound induces apoptosis of brain glioma in rats mediated by caspase-3, Bcl-2, and survivin

Zhang, Zhen; Jiang, Chen; Chen, Lufeng; Yang, Xianli; Zhong, Hongshan; Qi, Xun; Bi, Yonghua; Xu, Ke

doi:10.1016/j.brainres.2012.06.047

Cited by 24 publications

(21 citation statements)

References 20 publications

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“…Ultrasound-induced apoptosis is observed as a delayed biological effect in tissues exposed to high intensity ultrasound, especially in cell types that regenerate poorly such as neurons. Glioma cells exposed to ultrasound were shown to have increased caspase-3 expression and decreased expression of anti-apoptosis factors such as Bcl-2 and survivin leading to ultrasound induced cell death (106).…”

Section: Sonoporationmentioning

confidence: 99%

High Intensity Focused Ultrasound Technology, its Scope and Applications in Therapy and Drug Delivery

Phenix

Togtema²,

Pichardo³

et al. 2014

J Pharm Pharm Sci

123

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Ultrasonography is a safe, inexpensive and wide-spread diagnostic tool capable of producing real-time non-invasive images without significant biological effects. However, the propagation of higher energy, intensity and frequency ultrasound waves through living tissues can induce thermal, mechanical and chemical effects useful for a variety of therapeutic applications. With the recent development of clinically approved High Intensity Focused Ultrasound (HIFU) systems, therapeutic ultrasound is now a medical reality. Indeed, HIFU has been used for the thermal ablation of pathological lesions; localized, minimally invasive ultrasound-mediated drug delivery through the transient formation of pores on cell membranes; the temporary disruption of skin and the blood brain barrier; the ultrasound induced break-down of blood clots; and the targeted release of drugs using ultrasound and temperature sensitive drug carriers. This review seeks to engage the pharmaceutical research community by providing an overview on the biological effects of ultrasound as well as highlighting important therapeutic applications, current deficiencies and future directions.This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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Section: Sonoporationmentioning

confidence: 99%

High Intensity Focused Ultrasound Technology, its Scope and Applications in Therapy and Drug Delivery

Phenix

Togtema²,

Pichardo³

et al. 2014

J Pharm Pharm Sci

123

View full text Add to dashboard Cite

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“…Although reports showed that tumor cell death could be induced by low-frequency, low-intensity ultrasound and that the apoptotic pathways were caspase-3-dependent with or without the involvement of bax (a pro-apoptotic protein) and bcl-2 (an anti-apoptotic protein) (Bai et al, 2012;Zhang et al, 2012), we did not know if VSMCs respond like tumor cells. Besides, there are few reports about the effect of low-frequency ultrasound irradiation on apoptosis of VSMCs.…”

Section: Discussionmentioning

confidence: 82%

Low-frequency ultrasound induces apoptosis of rat aortic smooth muscle cells (A7r5) via the intrinsic apoptotic pathway

et al. 2014

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ABSTRACT. Ultrasound, a non-invasive therapy method, is a potential tool for medical applications, but its biological effects on vascular smooth muscle cells (VSMCs) have not been characterized. The aim of this study was to explore the effect and possible apoptotic mechanism of VSMCs that were induced by low-frequency ultrasound (LFU). Cell viability and apoptosis of A7r5 cells were evaluated after treating A7r5 cells with a continuous 45-kHz 1.0-W/cm 2 ultrasound (exposure time of 0, 10, 20, 30, and 35 s) by MTT assay and flow cytometry. At the optimum ultrasound exposure condition (30 s), gene chip analysis was performed, and the apoptotic signaling pathway was confirmed by reverse transcription-polymerase chain reaction and Western blot. As measured by flow cytometry, LFU significantly induced A7r5 cell apoptosis. Comparing the ultrasound group with the control group, the protein expression of caspase-9 and caspase-3 was increased by 50 and 57%, respectively; the caspase-3 mRNA level was increased by 37.5%. These findings indicate that an intrinsic pathway plays a major role in apoptosis that is induced by LFU and that LFU can induce A7r5 cell apoptosis via caspase-9-and caspase-3-dependent pathways.

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“…The down-regulation of caveolin-1 treated by caveolin-1 antibody or caveolin-1 knockdown dramatically suppressed prostate cancer cell growth in culture and in mouse model experiments, indicating that the role of caveolin-1 in prostate carcinoma was unique and caveolin-1 might be a potential therapeutic target for prostate cancer treatment [12,26,27]. Zhang et al [28] had reported that low frequency and intensity ultrasound could induce apoptosis in C6 brain glioma cells and C6 tumor-bearing rats, and affected the expression of three signaling proteins involved in apoptosis, including caspase-3, Bcl-2, and survivin. These findings proved that low frequency ultrasound could induce significantly biological effects that resulted in changes in protein expression in tumor cells.…”

Section: Discussionmentioning

confidence: 96%

Caveolin-1 as a biomarker to predict therapeutic effect of low-frequency ultrasound combined with SonoVue on prostate cancer in nude mice model

Nan

Bai

et al. 2014

CBM

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Low frequency and intensity ultrasound induces apoptosis of brain glioma in rats mediated by caspase-3, Bcl-2, and survivin

Cited by 24 publications

References 20 publications

High Intensity Focused Ultrasound Technology, its Scope and Applications in Therapy and Drug Delivery

High Intensity Focused Ultrasound Technology, its Scope and Applications in Therapy and Drug Delivery

Low-frequency ultrasound induces apoptosis of rat aortic smooth muscle cells (A7r5) via the intrinsic apoptotic pathway

Caveolin-1 as a biomarker to predict therapeutic effect of low-frequency ultrasound combined with SonoVue on prostate cancer in nude mice model

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