Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples

Vaisocherová‐Lísalová, Hana; Víšová, Ivana; Ermini, Maria Laura; Špringer, Tomáš; Song, Xue; Mrázek, Jan; Lamačová, Josefína; Lynn, N. Scott; Šedivák, Petr; Homola, Jiřı́

doi:10.1016/j.bios.2016.01.040

Cited by 197 publications

(112 citation statements)

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“…Through miniaturization and integration of various bioanalytical processes, microfluidic technologies have the potential to offer rapid, cost-effective, and portable solutions to medical needs in the developing world [10]. Within the application space of enteric screening, a variety of microfluidic detection modalities have been reported, including electrochemical [11,12], surface plasmon resonance (SPR) [13], and molecular methods such as polymerase chain reaction (PCR) [14,15,16] and loop-mediated isothermal amplification (LAMP) [17,18,19,20,21,22,23]. A notable subset of the microfluidic system types is centrifugal, or lab-on-a-disc, devices, first developed in the late 1990s [24].…”

Section: Introductionmentioning

confidence: 99%

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

et al. 2016

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Enteric and diarrheal diseases are a major cause of childhood illness and death in countries with developing economies. Each year, more than half of a million children under the age of five die from these diseases. We have developed a portable, microfluidic platform capable of simultaneous, multiplexed detection of several of the bacterial pathogens that cause these diseases. This platform can perform fast, sensitive immunoassays directly from relevant, complex clinical matrices such as stool without extensive sample cleanup or preparation. Using only 1 µL of sample per assay, we demonstrate simultaneous multiplexed detection of four bacterial pathogens implicated in diarrheal and enteric diseases in less than 20 min.

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Section: Introductionmentioning

confidence: 99%

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

et al. 2016

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“…can survive outside of their natural habitat, e.g., in water and food products (White et al, 2002). Salmonella detection by SPR-based assays with antibodies as the recognition element has been described in many studies (Bokken et al, 2003;Oh et al, 2004;Mazumdar et al, 2007;Dudak and Boyaci, 2009;Mazumdar et al, 2010;Singh et al, 2015;Vaisocherová-Lísalová et al, 2016).…”

Section: Salmonella Sppmentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

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Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

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“…Many methods have been reported for simultaneous detection of multi small molecular contaminants such as mycotoxins (Li et al, 2013(Li et al, , 2019Zhang et al, 2016;Wang et al, 2017a), pesticide residues (Bagheri et al, 2016;Wang et al, 2017b), and veterinary drugs (Taranova et al, 2015;Dasenaki et al, 2016;Zhu et al, 2016). Also, a lot of methods were described for simultaneous detection of multi microorganisms such as pathogenic bacteria (Li et al, 2015;Yoo et al, 2015;Vaisocherova-Lisalova et al, 2016), fungal pathogens (Playford et al, 2006;Priyanka et al, 2015;Rahn et al, 2016), and even varied pathogens that belong to different kingdoms (Leber et al, 2016). However, very few reported on how to simultaneously detect small molecular contaminants and microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration

et al. 2020

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Simultaneous detection technology has become a hot topic in analytical chemistry; however, very few reports on how to simultaneously detect small molecular contaminants and microorganisms have been in place. Aflatoxins are a group of highly toxic and carcinogenic compounds, which are produced mainly by Aspergillus flavus and Aspergillus parasiticus from section Flavi responsible for aflatoxin accumulation in stored cereals. Both aflatoxins and Aspergillus section Flavi were used to demonstrate the duplex real-time RCR method of simultaneously detecting small molecular contaminants and microorganisms. The detection of aflatoxins and Aspergillus section Flavi was carried out depending on the anti-idiotypic nanobody-phage V 2−5 and aflatoxin-synthesis related gene nor-1 (=aflD), respectively. The quantitative standard curves for simultaneous detection of aflatoxins and Aspergillus section Flavi were constructed, with detection limits of 0.02 ng/ml and 8 × 10 2 spores/g, respectively. Naturally contaminated maize samples (n = 25) were analyzed for a further validation. The results were in good agreement between the new developed method and the referential methods (high-performance liquid chromatography and the conventional plating counts).

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Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples

Cited by 197 publications

References 58 publications

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

Rapid, Portable, Multiplexed Detection of Bacterial Pathogens Directly from Clinical Sample Matrices

Biosensors and their Applications in Food Safety: A Review

Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration

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