Low fouling and ultrasensitive electrochemical immunosensors with dual assay methods based on Fe₃O₄ magnetic nanoparticles

Abstract

Low fouling electrochemical immunosensors for the highly sensitive detection of cancer antigen 15-3 with both “signal-off” and “signal-on” analytical methods were developed.

The Application of Prussian Blue Nanoparticles in Tumor Diagnosis and Treatment

“…The results showed that the amount of Fe 3 O 4 @Ag bonded to the electrode surface decreased with increasing concentration of CA 15-3, which is because of the competitive immunoreaction that causes a reduction in the detectable signals. A linear relationship between the current and the logarithm of the CA 15-3 concentration was obtained in the range of 10 to 1000 µU/mL, with a LOD of 5.2 µU/mL (Figure 2b) [70]. It is seen that the combination of the traditional electrode and a magnetic electrode could be a new strategy to generate and amplify the detecting signals for sensing.…”

Magnetically Stimulated Bio‐ and Electrochemical Systems: State‐of‐the‐Art, Applications, and Future Directions

“…For instance, electrochemical immunosensors able to quantify cancer antigen 15-3 in human serum were designed by using a mediated electrochemical reaction. [118] Antigens were conjugated on magnetic Fe 3 O 4 particles that were subsequently concentrated magnetically on the electrode. Electrochemical conversion of theses functional magnetic vehicles into Prussian blue enabled the transduction of targets in human serum by mediated electrochemical detection.…”

“…Therefore, several prominent developments in the fields of nucleid acid and immunosensors were achieved. For instance, electrochemical immunosensors able to quantify cancer antigen 15–3 in human serum were designed by using a mediated electrochemical reaction [118] . Antigens were conjugated on magnetic Fe 3 O 4 particles that were subsequently concentrated magnetically on the electrode.…”

A novel label-free electrochemical immunosensor for the detection of heat shock protein 70 of lung adenocarcinoma cell line following paclitaxel treatment using l-cysteine-functionalized Au@MnO2/MoO3 nanocomposites

“…In the literature, the common methodology and strategy to efficiently enhance signaling are to add nanomaterials, such as metal nanoparticles, 18 conductive carbon materials, 19–21 conductive polymers, 21 and metal oxides. 22,23…”