Low fluid shear stress promoted ciliogenesis via Dvl2 in hUVECs

Sheng, Xin; Sheng, Yan; Gao, Shuanglin; Fan, Fang; Wang, Junhua

doi:10.1007/s00418-020-01908-3

Cited by 6 publications

(3 citation statements)

References 63 publications

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“…However, when ECs were treated by the laminar shear stress at 5 dyn/cm 2 , the acetylated α-tubulin expression was located in the cytoplasm. These results suggested that laminar shear stress at 5 dyn/ cm 2 would regulate the primary cilium formation, which were similar to the results that laminar shear stress but not disturbed shear stress induced a cilia disassembly [44].…”

Section: Discussionsupporting

confidence: 87%

“…Interestingly, when Cx43 was overexpressed in ECs exposed to 5 dyn/cm 2 , the colocation between Cx43 and acetylated α-tubulin was detected in cytoplasm, suggesting that Cx43 might bind to tubulin to harm primary cilia assembly, which was similar to that in fibroblast cells [ 46 ]. However, in the previous study, Dvl2 was proposed to play a vital role in the assembly and disassembly of primary cilia under different shear stresses [ 44 ]. It needs to be explored further whether Dvl2 was involved in primary cilia regulation in the present study.…”

Section: Discussionmentioning

confidence: 99%

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Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress

Zhou,

et al. 2023

J Vasc Res

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Objectives: This study aimed to determine the function of Cx43 in the endothelial-to-mesenchymal transition (EndMT) process of endothelial cells (ECs) and to explore the potential signaling pathways underlying these functions. Methods: ECs were extracted from rat aorta. ECs were transfected with Cx43 cDNA and Cx43 siRNA and then exposed to 5 or 12 dyne/cm2. Immunofluorescence staining was used to detect the expression of SM22α, Cx43, and acetylated α-tubulin in ECs. Western blotting was used to detect the protein expression of α-SMA, CD31, Cx43, H1-calponin, Ift88, and p-smad3 in ECs. Results: The expression of αSMA, SM22α, and Cx43 was significantly increased, and CD31 was markedly decreased in ECs treated with laminar shear stress at 5 dyn/cm2. The Cx43 cDNA transfection could induce the expression of SM22α or H1-calponin and attenuate CD31 expression in ECs. Also, Cx43 overexpression harms cilia formation in ECs exposed to 5 dyn/cm2, accompanied with the regulated Ift88 and smad signaling. Conclusions: This study found that laminar shear stress at 5 dyn/cm2 would increase the expression of Cx43 to facilitate the EndMT process of ECs, associated with morphological changes in primary cilia and the decreased expression of Ift88 in ECs.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress

Zhou,

et al. 2023

J Vasc Res

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“…EC have motile cilia (Delgehyr et al, 2015) and are exposed to the CSF mechanical forces (Siyahhan et al, 2014;Ringers et al, 2020). It has been already demonstrated that mechanical forces, especially shear stress, affect ciliogenesis (Sheng et al, 2020) and potentially might affect the stem cell niche. For the homeostasis of CSF flow to be achieved, ependymal cells need to maintain ciliary and planar cell polarity during and after ciliogenesis as it has been shown by Yamada et al in hydrocephalus models, with an increase in CSF oscillation directly impedes normal cilia beating (Yamada et al, 2021).…”

Section: Ependymal Maturation and Their Metabolic Function In Neural ...mentioning

confidence: 99%

Metabolic regulation of the neural stem cell fate: Unraveling new connections, establishing new concepts

et al. 2022

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The neural stem cell niche is a key regulator participating in the maintenance, regeneration, and repair of the brain. Within the niche neural stem cells (NSC) generate new neurons throughout life, which is important for tissue homeostasis and brain function. NSCs are regulated by intrinsic and extrinsic factors with cellular metabolism being lately recognized as one of the most important ones, with evidence suggesting that it may serve as a common signal integrator to ensure mammalian brain homeostasis. The aim of this review is to summarize recent insights into how metabolism affects NSC fate decisions in adult neural stem cell niches, with occasional referencing of embryonic neural stem cells when it is deemed necessary. Specifically, we will highlight the implication of mitochondria as crucial regulators of NSC fate decisions and the relationship between metabolism and ependymal cells. The link between primary cilia dysfunction in the region of hypothalamus and metabolic diseases will be examined as well. Lastly, the involvement of metabolic pathways in ependymal cell ciliogenesis and physiology regulation will be discussed.

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Vangl2 participates in the primary ciliary assembly under low fluid shear stress in hUVECs

et al. 2021

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Low fluid shear stress promoted ciliogenesis via Dvl2 in hUVECs

Cited by 6 publications

References 63 publications

Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress

Cx43 Facilitates Mesenchymal Transition of Endothelial Cells Induced by Shear Stress

Metabolic regulation of the neural stem cell fate: Unraveling new connections, establishing new concepts

Vangl2 participates in the primary ciliary assembly under low fluid shear stress in hUVECs

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