Low expression of TCP1 (T-Complex 1) and PSMC1 (Proteasome 26S subunit, ATPase 1) in heterotopic ossification during ankylosing spondylitis

Zhong, Xiaolin; Qian, Bangping; Huang, Jiannan; Zhao, Shi-Zhou; Yao, Li; Qiu, Yong

doi:10.1080/21655979.2021.1975981

Cited by 2 publications

(2 citation statements)

References 40 publications

(54 reference statements)

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“…TCP1 is also involved in bone pathology, and specifically, Ankylosing Spondylitis (AS)-induced heterotopic ossification. The protein has been shown to be underexpressed in the bone marrow-derived mesenchymal stem cells of Ankylosing Spondylitis compared with controls [ 26 ]. NSD1 is yet another protein involved in bone pathology, but novel in the context of OA.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis

Müller

Lee

Zhi

et al. 2023

Life

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Osteoarthritis (OA) is a highly prevalent degenerative joint condition that disproportionately affects females. The pathophysiology of the disease is not well understood, which makes diagnosis and treatment difficult. Given the physical connection of synovial fluid (SF) with articular tissues, the SF’s composition can reflect relevant biological modifications, and has therefore been a focus of research. Previously, we demonstrated that extracellular vesicles isolated from the synovial fluid of OA patients carry different cargo (protein and miRNA) in a sex-specific manner. Given the increased prevalence and severity of OA in females, this study aims to identify differential protein content within the synovial fluid of female OA and non-osteoarthritic (non-OA) patients. We found that several proteins were differentially expressed in osteoarthritic females compared with age-matched controls. Presenilin, Coagulation Factor X, Lysine-Specific Demethylase 2B, Tenascin C, Leucine-Rich Repeat-Containing Protein 17 fragments, and T-Complex Protein 1 were negatively regulated in the OA group, with PGD Synthase, Tubulointerstitial Nephritis Antigen, and Nuclear Receptor Binding SET Domain Protein 1 positively regulated in the OA group. Database for Annotation, Visualization, and Integrated Discovery (DAVID) and QuickGO analyses established these proteins as significantly involved in many biological, cellular, and molecular processes. In conclusion, the protein content of female synovial fluid is altered in OA patients, which is likely to provide insights into gender-specific pathophysiology.

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Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis

Müller

Lee

Zhi

et al. 2023

Life

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“…The fold change (FC) denotes the ratio of the expressing levels between these 2 groups, while log2FC represents the FC with a log base of 2, and the P-value is the threshold of significant difference. P < 0.05 and |log2FC| > 0.2 [17] were utilized as thresholds to screen differentially expressed genes. Meanwhile, the "pheatmap" and "ggplot2" R language packages were employed to create the heat map and volcano map, respectively, to visualize the differences.…”

Section: Differential Expression Analysis Of Crsmentioning

confidence: 99%

Bioinformatics-based analysis of potential candidates chromatin regulators for immune infiltration in osteoarthritis

Wang¹,

Ou²,

Peng

et al. 2022

BMC Musculoskelet Disord

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Background Through the bioinformatics analysis to screen out the potential chromatin regulators (CRs) under the immune infiltration of osteoarthritis (OA), thus providing some theoretical support for future studies of epigenetic mechanisms under OA immune infiltration. Methods By integrating CRs and the OA gene expression matrix, we performed weighted gene co-expression network analysis (WGCNA), differential analysis, and further screened Hub genes by protein-protein interaction (PPI) analysis. Using the OA gene expression matrix, immune infiltration extraction and quantification were performed to analyze the correlations and differences between immune infiltrating cells and their functions. By virtue of these Hub genes, Hub gene association analysis was completed and their upstream miRNAs were predicted by the FunRich software. Moreover, a risk model was established to analyze the risk probability of associated CRs in OA, and the confidence of the results was validated by the validation dataset. Results This research acquired a total of 32 overlapping genes, and 10 Hub genes were further identified. The strongest positive correlation between dendritic cells and mast cells and the strongest negative correlation between parainflammation and Type I IFN reponse. In the OA group DCs, iDCs, macrophages, MCs, APC co-inhibition, and CCR were significantly increased, whereas B cells, NK cells, Th2 cells, TIL, and T cell co-stimulation were significantly decreased. The risk model results revealed that BRD1 might be an independent risk factor for OA, and the validation dataset results are consistent with it. 60 upstream miRNAs of OA-related CRs were predicted by the FunRich software. Conclusion This study identified certain potential CRs and miRNAs that could regulate OA immunity, thus providing certain theoretical supports for future epigenetic mechanism studies on the immune infiltration of OA.

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Low expression of TCP1 (T-Complex 1) and PSMC1 (Proteasome 26S subunit, ATPase 1) in heterotopic ossification during ankylosing spondylitis

Cited by 2 publications

References 40 publications

Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis

Proteomic Analysis of Female Synovial Fluid to Identify Novel Biomarkers for Osteoarthritis

Bioinformatics-based analysis of potential candidates chromatin regulators for immune infiltration in osteoarthritis

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