Low expression of miR-let-7a promotes cell growth and invasion through the regulation of c-Myc in oral squamous cell carcinoma

Luo, Chunyang; Zhang, Jiyong; Zhang, Yi; Zhang, Xiao; Chen, Yinan; Fan, Weimin

doi:10.1080/15384101.2020.1786633

Cited by 16 publications

(8 citation statements)

References 43 publications

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“…let-7a/Lin28B is a miRNA/protein axis that was first discovered in the nematode Caenorhabditis elegans, and has multiple functions in metastasis, tumorigenesis and cancer stem cell biology (24,45,46). let-7a has a low expression in numerous types of carcinomas, such as laryngeal squamous cell carcinoma (47), gastric carcinoma (48), colorectal carcinoma (49) and oral squamous cell carcinoma (50). The present results are in accordance with this regulatory mechanism, and the Figure 1.…”

Section: Discussionsupporting

confidence: 87%

Estrogen 17β‑estradiol accelerates the proliferation of uterine junctional zone smooth muscle cells via the let‑7a/Lin28B axis in adenomyosis

et al. 2021

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The estrogen 17β-estradiol has been proven to serve an indispensable role in the occurrence and development of adenomyosis (ADS). The let-7a/Lin28B axis can control cell proliferation by acting as a tumor-inhibiting axis in numerous types of cancer. However, its role in ADS remains unknown. The present study aimed i) to elucidate the role of let-7a in regulating the proliferation of human uterine junctional zone (JZ) smooth muscle cells (SMCs) in ADS, ii) to evaluate whether 17β-estradiol modifies the expression levels of let-7a and Lin28B in JZ SMCs in ADS, and iii) to establish how 17β-estradiol affects the function of the let-7a/Lin28B axis in the proliferation of JZ SMCs in ADS. A total of 36 premenopausal women with ADS were enrolled as the experimental group and 34 women without ADS were recruited as the control group. Reverse transcription-quantitative PCR was used to evaluate the expression level of let-7a, and western blotting was performed to determine the Lin28B expression levels. Lentiviral null vector, let-7a overexpression lentiviral vector GV280 and let-7a inhibition lentiviral vector GV369 were used to infect cells to alter the expression of let-7a for further functional experiments. 17β-estradiol and Cell Counting Kit-8 assays were conducted to determine how 17β-estradiol affects the function of the let-7a/Lin28B axis in the proliferation of JZ SMCs in ADS. The results demonstrated that let-7a was downregulated and Lin28B was upregulated in the JZ SMCs of ADS compared with the control cells (P<0.0001). Moreover, a lower expression of let-7a led to faster proliferation of JZ SMCs (P<0.05), and 17β-estradiol affected the let-7a/Lin28B axis to accelerate the proliferation of JZ SMCs in ADS (P<0.05). These data suggested that 17β-estradiol collaborates with the let-7a/Lin28B axis to affect the development of ADS.

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Section: Discussionsupporting

confidence: 87%

Estrogen 17β‑estradiol accelerates the proliferation of uterine junctional zone smooth muscle cells via the let‑7a/Lin28B axis in adenomyosis

et al. 2021

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“…Overexpression of let-7a also inhibited HeLa cell proliferation. Low expression of let-7a is associated with growth and invasion in oral squamous cell carcinoma [ 16 ]. Recurrent prostate cancer has decreased expression of let-7a [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Upregulated microRNA let-7a accelerates apoptosis and inhibits proliferation in uterine junctional zone smooth muscle cells in adenomyosis under conditions of a normal activated hippo-YAP1 axis

Huang

Duan

Wang

et al. 2021

Reprod Biol Endocrinol

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Background Let-7a is a small non-coding RNA that has been found to take part in cell proliferation and apoptosis. The hippo-YAP1 axis, known as a tumour suppressor pathway, also plays an important role in cell proliferation and apoptosis. YAP1, TAZ, and phospho-YAP1 play key roles in actions of the hippo-YAP1 axis. Adenomyosis (ADS) is a proliferative disease leading to a large uterus in patients with prolonged illness. Abnormal proliferation of smooth muscle cells (SMCs) in the uterine endometrial-myometrial junctional zone (JZ) is an important reason for developing ADS. This study aimed to explore the expression levels of let-7a and components of the hippo-YAP1 axis in SMCs in the uterine endometrial-myometrial JZ in ADS and to explore the roles of let-7a and the hippo-YAP1 axis of JZ SMC proliferation and apoptosis in ADS. Methods We collected JZ tissues for the primary culture of SMCs from 25 women diagnosed with ADS and 27 women without ADS. We used quantitative real-time polymerase chain reaction and western blotting to measure the mRNA and protein expression levels of let-7a, YAP1, TAZ, and phospho-YAP1 in ADS JZ SMCs. A CCK-8 assay and flow cytometry analysis of apoptosis were utilized to test the proliferation and apoptosis of JZ SMCs. The let-7a overexpression lentiviral vector GV280 was used to increase the expression level of let-7a. We added verteporfin to block the phosphorylation of components of the hippo-YAP1 axis. Results We found that the let-7a level was decreased, while the YAP1 and TAZ levels were increased in ADS JZ SMCs. Upregulated let-7a affected the expression levels of components of the hippo-YAP1 axis, accelerated apoptosis, and inhibited proliferation in JZ SMCs. Furthermore, accumulated YAP1 led to increasing proliferation of JZ SMCs after verteporfin treatment to block the phosphorylation of components of the hippo-YAP1 axis. If components of the hippo-YAP1 axis were unphosphorylated, upregulated let-7a could not inhibit the proliferation of ADS JZ SMCs. Upregulated let-7a could not activate the hippo-YAP1 axis in verteporfin treatment. Conclusions Our findings suggest that the let-7a and hippo-YAP1 axis may act as important regulators of JZ SMCs proliferation, and upregulated let-7a may be an effective method to treat ADS.

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“…In contrast, the level of miR-let 7a was significantly lower in moderately and poorly differentiated tumor, in extensive tumor dimensions and in lesions with lymph nodes involvement. Luo et al [47] analyzed the clinical significance of miR-let 7a expression in OSCC by examining the correlation of reduced miR-let 7a levels with clinicopathological features. They found that lower expression of miR-let 7a was significantly correlated only with larger tumor size but not with other clinical features.…”

Section: Discussionmentioning

confidence: 99%

Serum and Saliva Level of miR-31-5p and miR-let 7a in EBV Associated Oropharyngeal Cancer

Polz¹,

Morshed

Bibik³

et al. 2023

IJMS

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Epstein-Barr virus (EBV) has a well-documented association with head and neck neoplasms, including nasopharyngeal carcinoma (NPC). In the last few years, research aimed at elucidating the role of the miRs in the pathogenesis of head and neck cancer (HNC) has gained importance. The study of miRs expression has set new directions in the search for biomarkers with diagnostic and prognostic value, and even in the search for new therapeutic targets for various tumors, including HNC. The aim of current study was to approximate the importance of miR-31-5p and miR-let 7a in the pathogenesis of EBV associated oropharyngeal cancer. For this purpose, experiments were carried out to determine the level of mentioned miRs in serum among patients diagnosed with oropharyngeal cancer linked to EBV infection, depending on histological differentiation-grading (G1–G3) and TNM classification. All clinical specimens stratified by HPV status were HPV negative. The level of antibodies EBNA and EBVCA was also assessed. The obtained results showed a significantly increased serum level of miR-31-5p but decreased level of miR-let 7a in EBV positive oropharyngeal cancer patients. We demonstrated association between the level of tested miRs and clinical stage. Our findings showed that miR-31-5p and miR-let-7a may be involved in development and progression of EBV associated oropharyngeal cancer. Therefore, it seems important to further study these molecules, as well as to determine whether they could be important biomarkers in the diagnosis of oropharyngeal cancer associated with EBV infection.

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Low expression of miR-let-7a promotes cell growth and invasion through the regulation of c-Myc in oral squamous cell carcinoma

Cited by 16 publications

References 43 publications

Estrogen 17β‑estradiol accelerates the proliferation of uterine junctional zone smooth muscle cells via the let‑7a/Lin28B axis in adenomyosis

Estrogen 17β‑estradiol accelerates the proliferation of uterine junctional zone smooth muscle cells via the let‑7a/Lin28B axis in adenomyosis

Upregulated microRNA let-7a accelerates apoptosis and inhibits proliferation in uterine junctional zone smooth muscle cells in adenomyosis under conditions of a normal activated hippo-YAP1 axis

Serum and Saliva Level of miR-31-5p and miR-let 7a in EBV Associated Oropharyngeal Cancer

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