Low Expression of miR-491-3p Is Correlated with Lymph Node Metastasis in Gastric Cancer

Abstract

Objective. MiR-491-3p, as a tumor suppressor miRNA, was found decreased in many solid tissues. In this study, we aim to investigate miR-491-3p expression in gastric cancer with or without lymph node metastasis (LNM). Methods. GSE173215 dataset from Gene Expression Omnibus (GEO) was used to measure miRNA expression from tissue samples of gastric cancer patients. Moreover, gastric tumor tissues (non-LNM: n = 78; LNM: n = 68) were obtained to detect the miR-491-3p expression. Receiver operating characteristic (ROC) curve and Kaplan–Meier (KM) survival analysis, as well as Cox regression analysis, were performed to reveal the role of miR-491-3p in diagnosis and prognosis of gastric cancer. Results. According to GSE173215 datasets (t = −11.25, adjust P value = 1.30E-06) and our clinical results (0.390 ± 0.193 vs. 0.562 ± 0.166, P < 0.005 ), the gastric cancer patients with LNM showed lower miR-491-3p expression than those without LNM, demonstrating a high diagnostic efficiency (sensitivity: 74.36%; specificity: 69.12%). In addition, both LNM and low miR-491-3p expression were correlated with the poor prognosis of gastric cancer. Furthermore, the LNM patient with low expression of miR-491-3p had the worse prognosis, but the non-LNM patient with high expression of miR-491-3p had the best prognosis. MiR-491-3p expression (HR = 0.003, 95%CI: 3.35E-04∼0.028) and LNM (HR = 2.326, 95%CI: 1.046∼5.173) were independent risk factors for gastric cancer. Conclusion. Downregulated miR-491-3p expression was found in gastric cancer, being a high diagnostic efficiency and an independent risk factor for gastric cancer, especially in those having LNM.

Exosomal miR-224-3p promotes lymphangiogenesis and lymph node metastasis by targeting GSK3B in gastric cancer

“…LNM in GC is a complex and orderly process, involving the acquisition of invasive phenotype at the primary tumor site, preparation of metastasis channels, regulation of immune microenvironment, preparation of microenvironment at the metastasis site, and other pathological processes [5][6][7][8]. In-depth exploration and study of the pathological mechanism in the process of LNM would better understand the biological behavior of GC and provide more targets for the diagnosis and treatment [9,10].…”

Downregulated miRNA-491-3p accelerates colorectal cancer growth by increasing uMtCK expression

“…MiR-491-3p is one of the mature products of miR-491. Recently, miR-491-3p has been reported to be involved in the progression of several cancers, such as retinoblastoma, tongue cancer, glioblastoma, and gastric cancer ( Hu et al, 2021 ; Zheng et al, 2015 ; Li et al, 2015 ; Yu & Luo, 2022 ). Furthermore, it has been demonstrated that miR-491 facilitates CRC cell apoptosis by reducing Bcl-XL expression ( Nakano et al., 2010 ).…”

Retracted: Low Expression of miR‐491‐3p Is Correlated with Lymph Node Metastasis in Gastric Cancer

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Peer-review manipulation …”

“…Tis article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. Tis investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”